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Cuminaldehyde exhibits potential antibiofilm activity against Pseudomonas aeruginosa involving reactive oxygen species (ROS) accumulation: a way forward towards sustainable biofilm management

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Abstract

Pseudomonas aeruginosa often causes various acute and chronic infections in humans exploiting biofilm. Molecules interfering with microbial biofilm formation could be explored for the sustainable management of infections linked to biofilm. Towards this direction, the antimicrobial and antibiofilm activity of cuminaldehyde, an active ingredient of the essential oil of Cuminum cyminum was tested against Pseudomonas aeruginosa. In this regard, the minimum inhibitory concentration (MIC) of cuminaldehyde was found to be 150 μg/mL against the test organism. Experiments such as crystal violet assay, estimation of total biofilm protein, fluorescence microscopy and measurement of extracellular polymeric substances (EPS) indicated that the sub-MIC doses (up to 60 µg/mL) of cuminaldehyde demonstrated considerable antibiofilm activity without showing any antimicrobial activity to the test organism. Moreover, cuminaldehyde treatment resulted in substantial accumulation of cellular reactive oxygen species (ROS) that led to the inhibition of microbial biofilm formation. To this end, the exposure of ascorbic acid was found to restore the biofilm-forming ability of the cuminaldehyde-treated cells. Besides, a noticeable reduction in proteolytic activity was also observed when the organism was treated with cuminaldehyde. Taken together, the results demonstrated that cuminaldehyde could be used as a promising molecule to inhibit the biofilm formation of Pseudomonas aeruginosa.

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Availability of data and material

All data generated or analysed during this study are included in this article. The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Ms. Ahana Chatterjee for critically reading the manuscript. We are extremely grateful to Dr. Debasish Maity (Professor, Department of Human Physiology, Tripura University, India) for sharing the organism with us to carry out our research activities.

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Authors and Affiliations

  1. Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India

    Sudipta Chatterjee, Payel Paul, Poulomi Chakraborty, Sharmistha Das, Ranojit Kumar Sarker & Prosun Tribedi

  2. Department of Microbiology, School of Life Sciences, Swami Vivekananda University, Barrackpore, West Bengal, 7000121, India

    Subhasis Sarkar

  3. National Institute of Biomedical Genomics, Kalyani, West Bengal, 741251, India

    Amlan Das

Authors
  1. Sudipta Chatterjee
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  2. Payel Paul
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  3. Poulomi Chakraborty
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  4. Sharmistha Das
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  5. Ranojit Kumar Sarker
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  6. Subhasis Sarkar
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  7. Amlan Das
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  8. Prosun Tribedi
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Contributions

SC, PP, PC, SD, RKS performed the experiments and wrote the manuscript. SS analysed the results and wrote the manuscript. AD and PT conceived the idea, designed the experiments, analysed the results and edited the manuscript critically.

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Correspondence to Amlan Das or Prosun Tribedi.

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Chatterjee, S., Paul, P., Chakraborty, P. et al. Cuminaldehyde exhibits potential antibiofilm activity against Pseudomonas aeruginosa involving reactive oxygen species (ROS) accumulation: a way forward towards sustainable biofilm management. 3 Biotech 11, 485 (2021). https://doi.org/10.1007/s13205-021-03013-1

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