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1,4-Naphthoquinone accumulates reactive oxygen species in Staphylococcus aureus: a promising approach towards effective management of biofilm threat

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Abstract

Staphylococcus aureus, a Gram-positive opportunistic microorganism, promotes pathogenicity in the human host through biofilm formation. Microorganisms associated with biofilm often exhibit drug-resistance property that poses a major threat to public healthcare. Thus, the exploration of new therapeutic approaches is the need of the hour to manage biofilm-borne infections. In the present study, efforts are put together to test the antimicrobial as well as antibiofilm activity of 1,4-naphthoquinone against Staphylococcus aureus. The result showed that the minimum bactericidal concentration (MBC) of this compound was found to be 100 µg/mL against Staphylococcus aureus. In this regard, an array of experiments (crystal violet, biofilm protein measurement, and microscopic analysis) related to biofilm assay were conducted with the sub-MBC concentrations (1/20 and 1/10 MBC) of 1,4-naphthoquinone. All the results of biofilm assay demonstrated that these tested concentrations (1/20 and 1/10 MBC) of the compound (1,4-naphthoquinone) showed a significant reduction in biofilm development by Staphylococcus aureus. Moreover, the tested concentrations (1/20 and 1/10 MBC) of the compound (1,4-naphthoquinone) were able to reduce the microbial motility of Staphylococcus aureus that might affect the development of biofilm. Further studies revealed that the treatment of 1,4-naphthoquinone to the organism was found to increase the cellular accumulation of reactive oxygen species (ROS) that resulted in the inhibition of biofilm formation by Staphylococcus aureus. Hence, it can be concluded that 1,4-naphthoquinone might be considered as a promising compound towards biofilm inhibition caused by Staphylococcus aureus.

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Authors and Affiliations

  1. Microbial Ecology Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India

    Payel Paul, Poulomi Chakraborty, Ahana Chatterjee, Ranojit K. Sarker & Prosun Tribedi

  2. Guru Nanak Institute of Pharmaceutical Science and Technology, 157/F Nilgunj Road, Panihati, Kolkata, West Bengal, 700114, India

    Debabrata Ghosh Dastidar

  3. Department of Chemistry, NIT Sikkim, Ravangla Campus, Barfung Block, Ravangla, Sikkim, 737139, India

    Taraknath Kundu & Amlan Das

  4. School of Life Sciences, The Neotia University, Sarisha, West Bengal, 743368, India

    Niloy Sarkar

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  1. Payel Paul
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  2. Poulomi Chakraborty
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  3. Ahana Chatterjee
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  4. Ranojit K. Sarker
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Correspondence to Amlan Das or Prosun Tribedi.

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Communicated by Erko Stackebrandt.

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Paul, P., Chakraborty, P., Chatterjee, A. et al. 1,4-Naphthoquinone accumulates reactive oxygen species in Staphylococcus aureus: a promising approach towards effective management of biofilm threat. Arch Microbiol 203, 1183–1193 (2021). https://doi.org/10.1007/s00203-020-02117-1

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