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Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr

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Abstract

The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid-beta1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and in vitro neuroprotective cell viability, intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were evaluated with human neuroblastoma SH-SY5Y cells insulted with Aβ. Chromatographic separation on the alcoholic extract yielded known phytosterols. Results showed that pretreatment of the SH-SY5Y cells with the P. clementis extracts increased cell viability and MMP, and decreased ROS, suggesting protective effects. Hence, P. clementis extract has promising neuroprotective therapeutic potential.

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Acknowledgements

The National Research Foundation of Korea (NRF) Grants awarded by the Korean government (MES, No. 2020R1A2B5B01002463) is gratefully acknowledged for their financial support. The De La Salle University (Laguna Campus, Philippines) is gratefully acknowledged for the NMR measurements and the Commission on Higher Education for additional resources. We also thank Dr. Porferio S. Bangcaya for the plant collection and Patricia Marie Oliva for the technical help on the plant extraction.

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Authors and Affiliations

  1. College of Science and Research Center for the Natural and Applied Sciences, University of Santo Tomas, 1015, Manila, Philippines

    Mario A. Tan, Byron Leander U. Tan & Maribel G. Nonato

  2. Bionano Research Institute, Gachon University, Seongnam-si, Republic of Korea

    Seong Soo A. An

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  1. Mario A. Tan
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  2. Byron Leander U. Tan
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  3. Maribel G. Nonato
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  4. Seong Soo A. An
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Contributions

MAT and SSAN conceptualized the study; MAT and BLUT performed the experiments; SSAN and MGN for the resources; MAT wrote the manuscript; MGN and SSAN revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Mario A. Tan or Seong Soo A. An.

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The article does not contain any studies involving human participants or animals.

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Tan, M.A., Tan, B.L.U., Nonato, M.G. et al. Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr. 3 Biotech 11, 330 (2021). https://doi.org/10.1007/s13205-021-02889-3

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