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Production of nisin and lactic acid from the starch of sweet potato by simultaneous saccharification and fermentation with two stage pH adjustment

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Abstract

Nisin is an antimicrobial peptide which is widely used as preservative, while lactic acid is a natural organic acid applied in the food industry. The aim of this work was to study the process for nisin and lactic acid production from starch of sweet potato with simultaneous saccharification and fermentation (SSF) by Lactococcus lactis subsp. Lactis with two stage pH adjustment. The factors impacting the nisin and lactic acid production including starch concentration, glucosidase concentration, CaCO3 and Tween-80 were studied. The nisin titre reached a high of 2516.41 IU/mL, while the lactic acid reached a high of 37.06 g/L when the optimal conditions were 40 g/L starch, 100 U glucosidase/g starch, 2.5% CaCO3 and 1 mL/L Tween-80. The lactic acid and nisin were separated by a two stage pH adjustment at last. The SSF of starch from sweet potato coupled with a two stage pH adjustment is a promising method to produce nisin and lactic acid.

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Acknowledgements

We thank Renee Mosi, PhD, from Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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Author notes

  1. Yi Tong and Xiaodong Ren contributed equally to this work.

  2. Jin Tao contributed equally to this work with Qiyue Cheng.

Authors and Affiliations

  1. College of Life Science, Jilin University, Changchun, 130000, China

    Qiyue Cheng, Dongxu Li, Yan Liu, Xuyang Shi, Xintong Liu, Xiaoyun Zhang & Xiaodong Ren

  2. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130000, China

    Xiaodong Ren

  3. Jilin COFCO Bio-Chemical Co. Ltd., Changchun, 130000, China

    Jin Tao, Yi Li & Yi Tong

  4. National Engineering Research Center for Corn Deep Processing, Changchun, 130000, China

    Jin Tao, Yi Li & Yi Tong

  5. COFCO Nutrition and Health Research Institute, Beijing, 100000, China

    Wenzhao Li

Authors
  1. Qiyue Cheng
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  2. Jin Tao
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  3. Yi Li
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  4. Wenzhao Li
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  5. Dongxu Li
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  6. Yan Liu
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  9. Xiaoyun Zhang
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  10. Yi Tong
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  11. Xiaodong Ren
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Correspondence to Yi Tong or Xiaodong Ren.

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Cheng, Q., Tao, J., Li, Y. et al. Production of nisin and lactic acid from the starch of sweet potato by simultaneous saccharification and fermentation with two stage pH adjustment. 3 Biotech 11, 320 (2021). https://doi.org/10.1007/s13205-021-02778-9

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