Abstract
The Src homology 3 and cysteine-rich domain 3 gene (STAC3) encodes a protein containing both a cysteine-rich domain and two Src (sarcoma) homology 3 domains (SH3). STAC3 is specifically expressed in skeletal muscle and plays an important role in skeletal muscle development, but the explicit sequence and function of chicken SATC3 remain unknown. In the current study, we found the full-length chicken STAC3 cDNA to be 1383 bp long, with a 1092 bp open reading frame that harbors one cysteine-rich C1 domain and two SH3 domains. Tissue distribution analysis reveals chicken STAC3 mRNA only in skeletal muscle among 12 chicken tissues examined by reverse transcription PCR. Both cholecystokinin octapeptide analysis and a 5-ethynyl-2′-deoxyuridine assay suggest that neither STAC3 overexpression nor knockdown has any effect on the proliferation of chicken skeletal muscle satellite cells. However, STAC3 knockdown significantly increases the mRNA expression of MyoG, MyoD, Mb, and MyHC, and the protein abundance of MyHC and MyoG, whereas the opposite result is found in STAC3 overexpressed cells. We conclude that the STAC3 gene is expressed specifically in skeletal muscle and is a negative regulator of skeletal muscle satellite cell differentiation in chicken.
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Acknowledgements
This research was supported by Natural Science Foundation of China (31402069), China Agriculture Research System (CARS-40-K06) and Sichuan Science and Technology Program (2016NYZ0050). We thank Steven M. Thompson, from Liwen Bianji, Edanz Editing China (https://www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
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HY, JZ, and QZ performed the conceptualization; HY and JZ performed the methodology; HY and DL performed formal analysis; HY, JZ, SH, and CC performed investigation; QZ provided resources; HY wrote original draft preparation; HY and QZ wrote review and editing; QZ provided supervision; YW provided project administration; QZ and HY provided funding acquisition.
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All animal studies were approved by the Committee on Experimental Animal Management of Sichuan Agricultural University. The permit number is 2016-0AT070.
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Yin, H., Zhao, J., Han, S. et al. Molecular characterization, tissue distribution, and functional analysis of the STAC3 gene in chicken. 3 Biotech 10, 171 (2020). https://doi.org/10.1007/s13205-020-2161-2
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DOI: https://doi.org/10.1007/s13205-020-2161-2