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Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation

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Abstract

In this study, we identified three neuroparsin (NP) genes in Macrobrachium nipponense: Mn-NP1, Mn-NP2, and Mn-NP3, encoding 99, 100, and 101 amino acid proteins, respectively. Multiple sequence alignments showed that these genes contained 12 cysteine residues, of which 11 were at conserved positions. The total sequence identity between the genes was 47.5%, and they showed a high degree of sequence identity (> 54% similarity) with other crustacean genes. Phylogenetic tree analysis showed that Mn-NPs were clustered at different branches, indicating that Mn-NPs may have different functions. Tissue distribution data revealed that the three genes were present in males and females during the breeding and nonbreeding season, but their expression patterns differed. Mn-NP1 was highly expressed in the breeding season, in the male testis, and highly expressed in the nonbreeding season, in the female ovary. Mn-NP3 exhibited biased female expression in the breeding and nonbreeding season, with dominant expression in the ovary. All Mn-NPs were detected during embryo development, but with different expression patterns. These data indicated that Mn-NP1 may function during embryonic development, and that Mn-NP2 may be expressed during early embryo cell division, and late larval development. Mn-NP3 expression patterns reflected maternal inheritance, and may be associated with ovarian maturation. These expression data suggested that Mn-NP1 and Mn-NP2 are negatively correlated with ovarian development, with inhibition roles during this development. Mn-NP3 may be involved in vitellogenesis.

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Acknowledgements

This research was supported by Central Public interest Scientific Institution Basal Research Fund CAFS (2019JBFM04;2020TD36); Grants from the National Key R&D Progrom of China (2018YFD0901303); Jiangsu Agricultural Industry Technology System (JATS [2020] 461); The New cultivar breeding Major Project of Jiangsu province (PZCZ201745); the China Agriculture Research System-48 (CARS-48).

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Author notes

  1. Hui Qiao and Yiwei Xiong have contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, People’s Republic of China

    Hui Qiao, Yiwei Xiong, Sufei Jiang, Wenyi Zhang, Shubo Jin, Yongsheng Gong, Yan Wu & Hongtuo Fu

  2. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, People’s Republic of China

    Hongtuo Fu

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  1. Hui Qiao
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  2. Yiwei Xiong
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  4. Wenyi Zhang
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  5. Lei Xu
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  7. Yongsheng Gong
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  8. Yan Wu
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Correspondence to Hongtuo Fu.

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The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results

Appendix

Appendix

Figs. 8, 9, 10.

Fig. 8
figure 8

Nucleotide and deduced amino acid sequence of M. nipponense Neuroparsin 1 gene (Mn-NP1) cDNA. 3′UTR and 5′ UTR are listed with lowercase letters. ORF are showed by capital letters. The polyadenylation signal (aataa) is marked with double underline. The signal peptide is marked with single underline. Conserved cysteine residues are marked with shadow

Full size image
Fig. 9
figure 9

Nucleotide and deduced amino acid sequence of M. nipponense Neuroparsin 2 gene (Mn-NP2) cDNA. 3′UTR and 5′ UTR are listed with lowercase letters. ORF are showed by capital letters. The polyadenylation signal (aataa) is marked with double underline. The signal peptide is marked with single underline. Conserved cysteine residues are marked with shadow

Full size image
Fig. 10
figure 10

Nucleotide and deduced amino acid sequence of M. nipponense Neuroparsin 3 gene (Mn-NP3) cDNA. 3′UTR and 5′ UTR are listed with lowercase letters. ORF are showed by capital letters. The polyadenylation signal (aataa) is marked with double underline. The signal peptide is marked with single underline. Conserved cysteine residues are marked with shadow

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Qiao, H., Xiong, Y., Jiang, S. et al. Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation. 3 Biotech 10, 537 (2020). https://doi.org/10.1007/s13205-020-02531-8

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