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Cd-tolerant SY-2 strain of Stenotrophomonas maltophilia: a potential PGPR, isolated from the Nanjing mining area in China

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Abstract

Microbial and plant assisted bioremediation is an emerging way for the remediation of soils polluted with heavy metals. To screen the cadmium tolerant bacteria, soil samples were collected from Nanjing mining area, China. The average cadmium content of the mine soil reached 45.71 mg/kg, which was indicating serious pollution and potential ecological risk. From the mine soil, six cadmium tolerant plant growth-promoting rhizobacteria (PGPR) were isolated. The isolated bacterial strain “SY-2” showed maximum cadmium tolerance and it was selected for further experimentation. This strain was identified as Stenotrophomonas maltophilia by 16S rRNA gene sequencing (GenBank accession number MG597057). SY-2 was found to tolerate maximum cadmium at 1.0 mM concentration. This strain also exhibited good adsorption capacity (up to 35.7%) of heavy metal at 0.5 mM concentration. The results of this study exhibited organic phosphorus solubilization (37.08 mg/L) and IAA biosynthesis (15.11 mg/L) ability of isolated S. maltophilia. Scanning electron microscopy (SEM) revealed cell shrinkage and the cell wall of S. maltophilia was very rough. Moreover, the energy dispersive X-ray (EDX) analysis endorsed the adsorption of Cd ions on the surface of biomass. FT-IR study described the presence of functional groups and the nature of chemical bonds, before and after cadmium stress. At 0.25 mM cadmium concentration, S. maltophilia treated seeds of Capsicum annuum L. developed 1.46 times longer roots than untreated seeds. The results of this study helped us to conclude that SY-2 strain of S. maltophilia possesses significant metal tolerance and bioremediation potential against cadmium. In the future, this strain can be used as a microbial remediation agent to detoxify heavy metals in contaminated soils.

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Acknowledgements

The authors are highly thankful to the Instrumental Analysis Center of Shanghai Jiao Tong University for analyzing the heavy metal concentration, SEM-EDX and FT-IR.

Funding

Supported by research fund of Shanghai Landscape Architecture Construction Co., Ltd.

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shangai, 200240, China

    Fiza Liaquat

  2. Department of Architecture, Shanghai Jiao Tong University, Shangai, 200240, China

    Che Shengquan & Liu Qunlu

  3. Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Muhammad Farooq Hussain Munis & Urooj Haroon

  4. Department of Plant Sciences, Shanghai Jiao Tong University, Shangai, 200240, China

    Samiah Arif

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  1. Fiza Liaquat
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  2. Muhammad Farooq Hussain Munis
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  3. Samiah Arif
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  4. Urooj Haroon
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  5. Che Shengquan
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  6. Liu Qunlu
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Contributions

LQL designed the experiments and supervised the research work. LJK executed the experiments. FL analyzed the data and wrote the manuscript. MFHM supervised manuscript write up and submission. UH collected samples, SA did data compilation and CSQ supervised the research work. All authors read and approved the final manuscript.

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Correspondence to Liu Qunlu.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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MG597057 (Stenotrophomonas maltophilia).

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Liaquat, F., Munis, M.F.H., Arif, S. et al. Cd-tolerant SY-2 strain of Stenotrophomonas maltophilia: a potential PGPR, isolated from the Nanjing mining area in China. 3 Biotech 10, 519 (2020). https://doi.org/10.1007/s13205-020-02524-7

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