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Purification, characterization and cloning of a chitinase from Stenotrophomonas rhizophila G22

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Abstract

In the presented research the extracellular chitinase of Stenotrophomonas rhizophila G22 was biochemically and molecularly characterized. The studied enzyme was purified from a 72-h bacterial culture about 14 times, with a recovery of 63%. The molecular weight of the purified protein was estimated at 50 kDa by SDS-PAGE. The enzyme showed high activity against colloidal chitin. Significantly lower activities were observed with native chitin powder and chitosan. Adsorption of the enzyme to colloidal chitin and to powdered chitin at the level of 75% and 37%, respectively, was observed after 30 min of reaction. Optimum temperature and pH were 37 °C and 5.9, respectively. The enzyme demonstrated higher activity against nitrophenyl-β d N, N′, N″-triacetylchitotriose and approx. 5 times lower activity for 4-nitrophenyl-N, N′-diacetyl-β-d-chitobiose. The enzyme is an endochitinase, which is confirmed by the Km and Vmax values determined in the studies. S. rhizophila G22 endochitinase was inhibited in the presence of cysteine-specific inhibitors, which indicates the role of cysteine moieties in the mechanism of catalysis or in stabilisation of the enzyme molecule. Also Ca2+ and Mn2+ ions may stabilise the protein's spatial structure. SDS and ions: Fe2+, Cu2+, Co2+, Zn2+ inhibited the activity of enzyme. A full-length (2109 bp) gene coding chitinase from S. rhizophila G22 was obtained. Four domains typical for glycoside hydrolase family 18 (GH 18) chitinases were identified: catalytic Gly_18, chitin-binding—ChtBD3, type-III fibronectin—FN3 and polycystic kidney disease domain—PKD domain.

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Acknowledgments

This research was financed by the statutory activity of the Department Biochemistry, Warsaw University of Life Sciences—SGGW

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02787, Warsaw, Poland

    Urszula Jankiewicz & Bartosz Baranowski

  2. Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87100, Toruń, Poland

    Maria Swiontek Brzezinska

  3. Department of Remote Sensing and Environmental Assessment, Institute of Environmental Engineering, Warsaw University of Life Science, Nowoursynowska 159, 02787, Warsaw, Poland

    Magdalena Frąk

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  1. Urszula Jankiewicz
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  2. Bartosz Baranowski
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  3. Maria Swiontek Brzezinska
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  4. Magdalena Frąk
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Contributions

UJ, BB and MSB and MF designed the study. UJ, BB and MSB performed the research. UJ analysed the data and wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Urszula Jankiewicz.

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All authors declare that they have no conflict of interest. No grants were received.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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16S rRNA gene sequence for G22 bacteria was deposited in the DDBJ/EMBL/GenBank with the number: LC494522. The gene sequence of chitinase was deposited in the DDBJ/EMBL/GenBank with the number: LC497040.

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Jankiewicz, U., Baranowski, B., Swiontek Brzezinska, M. et al. Purification, characterization and cloning of a chitinase from Stenotrophomonas rhizophila G22. 3 Biotech 10, 16 (2020). https://doi.org/10.1007/s13205-019-2007-y

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