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Complete genome sequence analysis of Narcissus yellow stripe virus infecting Narcissus tazetta in India

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Abstract

The complete genome sequence of Narcissus yellow stripe potyvirus (NYSV) isolated from Narcissus tazetta cv. Paperwhite exhibiting leaf chlorotic stripe symptoms was determined for the first time from India. The viral genome sequence contained 9650 nucleotides that encode a large polyprotein (372.36 kDa) of 3103 amino acids. The comparison of the NYSV genome sequences with corresponding sequences of other potyviruses revealed 90–97% identities and closest phylogenetic relationships with NYSV-Zhangzhou-1 and -ZZ-2 isolates infecting N. tazetta reported from China. Therefore, the NYSV isolate understudy was considered as a new member of NYSV and designated as NYSV–NAR2.

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Acknowledgements

Authors are thankful to the Director, CSIR-NBRI, Lucknow, India for facilities. RR is thankful to University Grant Commission for Rajiv Gandhi National fellowships and AcSIR for Ph.D. registration. This study was funded by the In-house project OLP105.

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Authors and Affiliations

  1. Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research, National Botanical Research Institute, Lucknow, UP, 226001, India

    Rashmi Raj, Susheel Kumar & S. K. Raj

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, 201002, India

    Rashmi Raj, P. S. Chauhan & S. K. Raj

  3. Microbial Technology Division, Council of Scientific and Industrial Research, National Botanical Research Institute, Lucknow, UP, 226001, India

    Lalit Agrawal & P. S. Chauhan

  4. Department of Agriculture and Allied Sciences, Doon Business School, Dehradun, 248001, India

    Lalit Agrawal

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  1. Rashmi Raj
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  4. P. S. Chauhan
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  5. S. K. Raj
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Correspondence to S. K. Raj.

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Raj, R., Agrawal, L., Kumar, S. et al. Complete genome sequence analysis of Narcissus yellow stripe virus infecting Narcissus tazetta in India. 3 Biotech 9, 409 (2019). https://doi.org/10.1007/s13205-019-1939-6

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