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Complex gene response of herbicide-resistant Enterobacter strain NRS-1 under different glyphosate stresses

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Abstract

Knowledge of biological evolution and genetic mechanisms is gained by studying the adaptation of bacteria to survive in adverse environmental conditions. In this regard, transcriptomic profiling of a glyphosate-tolerant Enterobacter strain NRS-1 was studied under four different treatments to investigate the gene-regulatory system for glyphosate tolerance. A total of 83, 83, 60 and 74 genes were up-regulated and 108, 87, 178 and 117 genes down-regulated under 60-NPG, 110-NPG, NaCl (355 mM) and HCl (pH 4.46) stress treatments, respectively. Complex gene network was identified to be involved in regulating tolerance to glyphosate. This study revealed that NRS-1 has gained glyphosate tolerance at the cost of osmotic and acidic resistance. The 25 differentially expressed genes are reported to may have partly changed the function for providing resistance to glyphosate directly, among them genes metK, mtbK, fdnG and wzb that might detoxify/degrade the glyphosate. However, under 110-NPG condition, NRS-1 might have utilized economical and efficient ways by depressing its metabolism and activity to pass through this stress. Hence, the present study provides insights into the genes involved in glyphosate tolerance, which can be effectively utilized to engineer herbicide-resistant crop varieties after their proper validation to manage weed growth.

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Acknowledgements

This work was supported by grants from the MOA Special Project for Crop Breeding (2016ZX08004001), the Natural Science Foundation of China (31571691), the Fundamental Research Funds for the Central Universities (KYT201801), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55) and the Jiangsu JCIC-MCP program.

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  1. Soybean Research Institute, Nanjing Agricultural University, Weigang 1 Hao, Xuanwu District, Nanjing, 210095, Jiangsu, People’s Republic of China

    Yun-Yan Fei, Javaid Akhter Bhat, Ying-Ying Zhang, G. M. Al Amin, Jun-Yi Gai & Tuan-Jie Zhao

  2. National Center for Soybean Improvement, Nanjing, 210095, People’s Republic of China

    Yun-Yan Fei, Javaid Akhter Bhat, Ying-Ying Zhang, G. M. Al Amin, Jun-Yi Gai & Tuan-Jie Zhao

  3. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing, 210095, People’s Republic of China

    Yun-Yan Fei, Javaid Akhter Bhat, Ying-Ying Zhang, G. M. Al Amin, Jun-Yi Gai & Tuan-Jie Zhao

  4. Key Laboratory of Biology and Genetic Improvement of Soybean, Nanjing, 210095, People’s Republic of China

    Yun-Yan Fei, Javaid Akhter Bhat, Ying-Ying Zhang, G. M. Al Amin, Jun-Yi Gai & Tuan-Jie Zhao

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13205_2018_1455_MOESM1_ESM.pdf

Growth tendency of the bacteria under different stresses. (A) Growth curves of Enterobacter NRS-1 strain under stress. (B) Growth curves of wild-type Enterobacter strain under stress. The stresses used were 60-NPG, 110-NPG, NaCl (430 mM) and HCl (pH=3.9). Control samples received no stress treatment (PDF 300 KB)

13205_2018_1455_MOESM2_ESM.pdf

Gene Ontology (GO) classification of differentially expressed genes (DEGs) under four different stresses. (A) GO analysis of DEGs under NaCl (355 mM). (B) GO analysis of DEGs under HCl (pH=4.46). (C) GO analysis of DEGs under 60-NPG; (D) GO analysis of DEGs under 110-NPG glyphosate (PDF 826 KB)

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Fei, YY., Bhat, J.A., Zhang, YY. et al. Complex gene response of herbicide-resistant Enterobacter strain NRS-1 under different glyphosate stresses. 3 Biotech 8, 422 (2018). https://doi.org/10.1007/s13205-018-1455-0

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