Studies on growth dynamics of embryogenic cell suspension cultures of commercially important Indica rice cultivars ASD16 and Pusa basmati
Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures were initiated from 3-week-old embryogenic calli derived from mature seeds of Indica rice cultivars ASD16 and Pusa basmati. Growth kinetics of the rice cell suspensions were measured and the obtained data reveal that viable cells at 3% PCV using 20 ml liquid (N6D) medium and sub-culturing at 7-day intervals resulted in rapid increase in fresh and dry weights and the embryogenic competency of the cells were found to be high. The growth kinetics analysis revealed that ASD16 showed better efficiency for high frequency and viable somatic embryo formation as compared to Pusa basmati. The technique was found to be suitable for developing somatic embryos for both cultivars ASD16 and Pusa basmati, which can be used for many important applications including micropropagation and secondary metabolites production.
KeywordsRice Somatic embryogenesis Packed cell volume Cell viability
Sundararajan Sathish thanks Bharathiar University for University Research Fellowship. We would also like to thank, UGC-SAP and DST-FIST for the financial support to carry out this research.
SS designed the work, executed experiments and prepared the manuscript. VR did the experimental analysis. SN contributed substantially to the manuscript. RS mobilized the funds and critically evaluated the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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