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Efficient crude multi-enzyme produced by Trichoderma reesei using corncob for hydrolysis of lignocellulose

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Abstract

To improve the efficiency of enzymatic saccharification for lignocellulose, an efficient crude multi-enzyme was produced by Trichoderma reesei using corncob, a low cost inducer. Expression of cbh1, bgl1, egl1, xyn1 and positive regulator xyr1 induced by corncob increased significantly compared to that by cellulose. After 120 h induction by corncob, enzymatic activities on filter, CMC, β-glucose and xylan increased 86.5, 46.9, 120.9 and 291.2% compared to those induced by cellulose, and the concentration of secreted protein increased by 120.8%. FPase:β-glucosidase and FPase:xylanase values in crude multi-enzyme I (ECI, induced by corncob) were higher than that in crude multi-enzyme II (ECII, induced by cellulose). Under the same hydrolysis conditions, the volume dosage of ECI was only half of ECII, but ECI still showed a maximum of 12.5 and 33.4% higher than ECII in the total reducing sugar and glucose yield in lignocellulose hydrolysis. Corncob could be a candidate for low cost production of multi-enzyme for efficient lignocellulose degradation, and this work could guide the genetic modification of T. reesei to obtain efficient multi-enzyme for lignocellulose hydrolysis.

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References

  • Adav SS, Ravindran A, Sze SK (2012) Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass. J Proteomics 75:1493–1504

    Article  CAS  Google Scholar 

  • Alriksson B, Rose SH, van Zyl WH, Sjöde A, Nilvebrant N, Jönsson LJ (2009) Cellulase production from spent lignocellulose hydrolysates by recombinant Aspergillus niger. Appl Environ Microbiol 75:2366–2374

    Article  CAS  Google Scholar 

  • Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257–270

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268

    CAS  Google Scholar 

  • He J, Wu A, Chen D, Yu B, Mao X, Zheng P, Yu J, Tian G (2014) Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium. Biotechnol Biofuels 7:43–52

    Article  Google Scholar 

  • Herold S, Bischof R, Metz B, Seiboth B, Kubicek CP (2013) Xylanase gene transcription in Trichoderma reesei is triggered by different inducers representing different hemicellulosic pentose polymers. Eukaryot Cell 12:390–398

    Article  CAS  Google Scholar 

  • Herpoël-Gimbert I, Margeot A, Dolla A, Jan G, Mollé D, Lignon S, Mathis H, Sigoillot JC, Monot F, Asther M (2008) Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains. Biotechnol Biofuels 1:18–30

    Article  Google Scholar 

  • Jurgens G, Survase S, Berezina O, Sklavounos E, Linnekoski J, Kurkijärvi A, Väkevä M, van Heiningen A, Granström T (2012) Butanol production from lignocellulosics. Biotechnol Lett 34:1415–1434

    Article  CAS  Google Scholar 

  • Karaffa L, Fekete E, Gamauf C, Szentirmai A, Kubicek CP, Seiboth B (2006) d-Galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates. Microbiology 152:1507–1514

    Article  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  Google Scholar 

  • Li Q, Gao Y, Wang H, Li B, Liu C, Yu G, Mu X (2012) Comparison of different alkali-based pretreatments of corn stover for improving enzymatic saccharification. Bioresour Technol 125:193–199

    Article  CAS  Google Scholar 

  • Liao H, Li S, Wei Z, Shen Q, Xu Y (2014) Insights into high-efficiency lignocellulolytic enzyme production by Penicillium oxalicum GZ-2 induced by a complex substrate. Biotechnol Biofuels 7:162–178

    Article  Google Scholar 

  • Ling M, QinY Li N, Liang Z (2009) Binding of two transcriptional factors, Xyr1 and ACEI, in the promoter region of cellulase cbh1 gene. Biotechnol Lett 31:227–231

    Article  CAS  Google Scholar 

  • Liu D, Li J, Zhao S, Zhang R, Wang M, Miao Y, Shen Y, Shen Q (2013) Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources. Biotechnol Biofuels 6:149–165

    Article  CAS  Google Scholar 

  • Mandels M, Reese ET (1960) Induction of cellulase in fungi by cellobiose. J Bacteriol 79:816–826

    CAS  Google Scholar 

  • Merino ST, Cherry J (2007) Progress and challenges in enzyme development for biomass utilization. Adv Biochem Eng Biotechnol 108:95–120

    CAS  Google Scholar 

  • Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Ng IS, Tsai SW, Ju YM, Yu SM, Ho TD (2011) Dynamic synergistic effect on Trichoderma reesei cellulases by novel β-glucosidases from Taiwanese fungi. Bioresour Technol 102:6073–6081

    Article  CAS  Google Scholar 

  • Ouyang J, Yan M, Kong D, Xu L (2006) A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei. Biotechnol J 1:266–1274

    Google Scholar 

  • Payne CM, Knott BC, Mayes HB, Hansson H, Himmel ME, Sandgren M, Ståhlberg J, Beckham GT (2015) Fungal cellulases. Chem Rev 115:1308–1448

    Article  CAS  Google Scholar 

  • Peterson R, Nevalainen H (2012) Trichoderma reesei RUT-C30—30 years of strain improvement. Microbiology 158:58–68

    Article  CAS  Google Scholar 

  • Portnoy T, Margeot A, Seidl-Seiboth V, Le Crom S, Ben Chaabane F, Linke R, Seiboth B, Kubicek CP (2011) Differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE1 in Trichoderma reesei strains producing high and low levels of cellulase. Eukaryot Cell 10:262–271

    Article  CAS  Google Scholar 

  • Pribowo A, Arantes V, Saddler JN (2012) The adsorption and enzyme activity profiles of specific Trichoderma reesei cellulase/xylanase components when hydrolyzing steam pretreated corn stover. Enzyme Microb Technol 50:195–203

    Article  CAS  Google Scholar 

  • Sluiter A (2008) Determination of structural carbohydrates and lignin in biomass. In: Laboratory Analytical Procedure. National renewable energy laboratory, Golden, Colardo

  • Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11

    Article  CAS  Google Scholar 

  • Xia L, Shen X (2004) High-yield cellulase production by Trichoderma reesei ZU-02 on corncob residue. Bioresour Technol 91:259–262

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the Key Project of Natural Science Foundation of Tianjin (16JCZDJC31800), and the National Natural Science Fund of China (31770625).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Fengchao Jiang, Lijuan Ma, Rui Cai, Qing Ma, Gaojie Guo, Liping Du & Dongguang Xiao

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  1. Fengchao Jiang
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  2. Lijuan Ma
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  3. Rui Cai
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  4. Qing Ma
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  5. Gaojie Guo
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  6. Liping Du
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  7. Dongguang Xiao
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Correspondence to Lijuan Ma or Dongguang Xiao.

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Ethical approval

This study was focused on analyzing the efficient crude multi-enzyme produced by Trichoderma reesei using corncob for the hydrolysis of lignocellulose. Every parts of the research did not involve human participants and other animals. Our manuscript complies with the Ethical Rules applicable for 3 Biotech.

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The authors have declared that no conflicts of interest exist.

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Jiang, F., Ma, L., Cai, R. et al. Efficient crude multi-enzyme produced by Trichoderma reesei using corncob for hydrolysis of lignocellulose. 3 Biotech 7, 339 (2017). https://doi.org/10.1007/s13205-017-0982-4

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