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A sequential pretreatment of lignocelluloses in bamboo biomass to fermentable sugars by acid/enzymatic hydrolysis

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Abstract

A sequential pretreatment method for hydrolyzing rigid hemicelluloses and cellulose content in the bamboo biomass was investigated in this study. The effects of different parameters, such as nature of biomass, type of acid, acid and biomass concentration, were studied. Under the optimum condition of 5% (v/v) HCl-treated biomass and biomass concentration (8%, w/v), the maximum yield of sugar (619 mg/g of biomass) was obtained. The enzymatic hydrolysis parameter conditions were further optimized by response surface methodology-based central composite method. According to the results, the highest yield of sugar (515 mg/g of biomass) was obtained at hydrolysis temperature 50 °C, biomass concentration 8.9%, w/v, enzyme concentration (199.8 mg/g of biomass) and time 60 h, respectively. The effects of untreated, pretreated and enzymatically hydrolyzed biomass structure and complexity were investigated by field emission scanning electron microscopy and X-ray diffraction techniques.

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Acknowledgements

The authors acknowledge and thank the Management of SRM University and Director (E&T) for their support in carrying out this research work and also the Department of Chemical Engineering for providing necessary facilities.

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Authors and Affiliations

  1. Department of Chemical Engineering, SRM University, Chennai, Tamilnadu, 603203, India

    Praveenkumar Jagannathan & Krishnamurthi Tamilarasan

  2. Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamilnadu, 641013, India

    Chandrasekaran Muthukumaran

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  1. Praveenkumar Jagannathan
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  2. Chandrasekaran Muthukumaran
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  3. Krishnamurthi Tamilarasan
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Correspondence to Krishnamurthi Tamilarasan.

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Jagannathan, P., Muthukumaran, C. & Tamilarasan, K. A sequential pretreatment of lignocelluloses in bamboo biomass to fermentable sugars by acid/enzymatic hydrolysis. 3 Biotech 7, 260 (2017). https://doi.org/10.1007/s13205-017-0892-5

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  • DOI: https://doi.org/10.1007/s13205-017-0892-5

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