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Solid bioprocess of tarbush (Flourensia cernua) leaves for β-glucosidase production by Aspergillus niger: initial approach to fiber–glycoside interaction for enzyme induction

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Abstract

Commercial cellulase production has increased in recent years and consistent research has been carried out to improve levels of β-glucosidase. Bioprocesses have been successfully adapted to produce this enzyme, with solid-state fermentations as the best-suited technique involving fungi. The aim of this study was to use leaves of tarbush (Flourensia cernua), an abundant shrub of the Chihuahuan Desert, as a carbon source for β-glucosidase production by Aspergillus niger. During the solid bioprocess, this enzyme reached its peak production at 36 h of culture with 3876.6 U/L. There is a particular interest in the substrate composition because of the possibility of phenolic glycosides having an important role in β-glucosidase production. HPLC–MS analyses showed that glycosides were present with the highest accumulation at 36 h of fungal culture. Luteolin and apigenin glycosides [1.8 and 2.4 absorbance units, respectively] were also detected and showed their highest point of detection alongside the highest β-glucosidase activity. No apparent changes in cellulose were observed, while hemicellulose content decreased, which could be related to production and activity of β-glucosidase. This study shows that leaves of F. cernua are an important raw material for β-glucosidase production and give a source of compounds of added value which also may have an important role for β-glucosidase production.

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Acknowledgements

The authors would like to thank the National Council of Science and Technology (CONACyT) for providing a scholarship and financial support.

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Authors and Affiliations

  1. Departamento de Nutrición Animal, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro 1923, Buenavista, 25315, Saltillo, Coahuila, Mexico

    Juan Lopez-Trujillo, Miguel A. Medina-Morales, Ariel Sanchez-Flores, Carlos Arevalo, Miguel Mellado & Antonio F. Aguilera-Carbo

  2. División de Ingeniería, Departmento de Ciencias Básicas, Universidad Autónoma Agraria Antonio Narro, Saltillo, Mexico

    Juan Lopez-Trujillo

  3. Departmento de Investigación en Alimentos, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Mexico

    Juan A. Ascacio-Valdes & Cristobal N. Aguilar

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  1. Juan Lopez-Trujillo
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  2. Miguel A. Medina-Morales
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  5. Juan A. Ascacio-Valdes
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  7. Cristobal N. Aguilar
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  8. Antonio F. Aguilera-Carbo
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Correspondence to Antonio F. Aguilera-Carbo.

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Lopez-Trujillo, J., Medina-Morales, M.A., Sanchez-Flores, A. et al. Solid bioprocess of tarbush (Flourensia cernua) leaves for β-glucosidase production by Aspergillus niger: initial approach to fiber–glycoside interaction for enzyme induction. 3 Biotech 7, 271 (2017). https://doi.org/10.1007/s13205-017-0883-6

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