Abstract
Zinc oxide nanoparticles and curcumin are excellent antimicrobial agents. They have the potential to be used as alternative to antibiotics in wound infection management. In this study, ZnO–curcumin nanocomposite was synthesized and characterized. Physical adsorption of the nanocomposite onto collagen skin wound dressing was conducted and structural investigation was carried out by SEM. Antimicrobial assay, minimum inhibitory concentration (MIC), and viability assays of different concentrations of nanocomposite loaded collagen membrane were conducted against clinically isolated methicillin-resistant coagulase-negative Staphylococci (MRCoNS), such as S. epidermidis, S. hemolyticus, and S. saprophyticus. The nanocomposite showed excellent anti-CoNS activity on time kill assay with the MIC value of 195 µg/mL against S. epidermidis, S. hemolyticus and 390 µg/mL against S. saprophyticus. The nanocomposite loaded collagen membrane also showed excellent in vitro antistaphylococcal activity. This study may lead to the development of antibiotic alternate strategies to control and limit the MRCoNS in wound-related infections.
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Acknowledgements
This work was supported by the Indian Council of Medical Research, Government of India for the funded project on Coagulase-negative staphylococci. We acknowledge Director, School of Chemical Science and School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, for the help. We also thank the Department of Biotechnology, Government of India, for DBT RGYI and DBT—MSUB—IPLSARE Programs in School of Biosciences, Mahatma Gandhi University for providing instrumentation facilities. We are grateful to the Dean and laboratory staffs of the MOSC Medical College, Kolenchery.
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Soumya, K.R., Snigdha, S., Sugathan, S. et al. Zinc oxide–curcumin nanocomposite loaded collagen membrane as an effective material against methicillin-resistant coagulase-negative Staphylococci. 3 Biotech 7, 238 (2017). https://doi.org/10.1007/s13205-017-0861-z
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DOI: https://doi.org/10.1007/s13205-017-0861-z