Abstract
This study aims to synthesize silver nanoparticles (AgNPs) using Halimeda macroloba extract and assess their anticancer and photocatalytic properties. Ultraviolet–visible spectrophotometry (UV–visible), Fourier transform infrared (FT-IR) spectroscopy, Transmission electron microscope (TEM), scanning electron microscope-energy-dispersive spectroscopy (SEM–EDX), X-ray photoelectron spectroscopy (XPS), and particle-size distribution (PSD) were used to characterize the synthesized AgNPs. The green-synthesized AgNPs to be spherical in shape with a size of about 50–100 nm. The half-maximal inhibitory concentration (IC50) for AgNPs was determined to be 89.5 g/mL against the human hepatoma cell line (Huh-7). Fluorescent microscopy was used to evaluate the morphological alterations of Huh-7 cells stained with acridine orange/ethidium bromide (AO/EtBr), 2′-7′dichlorofluorescin diacetate (DCFH-DA), and rhodamine 123. Meanwhile, AgNPs exhibited 91.35% photocatalytic activity against methylene blue (MB) after 100 min of exposure to sunlight. Therefore, AgNPs have a strong potential for usage in anticancer activities and photocatalytic organic pollutant destruction.
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Ajay S, Panicker JS, Manjumol KA, Parameswaran P (2022) Subramanian, Photocatalytic activity of biogenic silver nanoparticles synthesized using Coleus Vettiveroids. Inorg Chem Commun 144:109926
Aksomaityte G, Poliakoff M, Lester E (2013) The production and formulation of silver nanoparticles using continuous hydrothermal synthesis. Chem Eng Sci 85:2–10. https://doi.org/10.1016/j.ces.2012.05.035
Ameen F, Srinivasan P, Selvankumar T et al (2019) Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity. Bioorg Chem 88:102970. https://doi.org/10.1016/j.bioorg.2019.102970
Ameen F, AlYahya S, Govarthanan M et al (2020) Soil bacteria Cupriavidus sp. mediates the extracellular synthesis of antibacterial silver nanoparticles. J Mol Struct. https://doi.org/10.1016/j.molstruc.2019.127233
Aravind M, Kumaresubitha T, Ahmed N, Velusamy P (2022) DFT, molecular docking, photocatalytic and antimicrobial activity of coumarin enriched Cinnamon bark extract mediated silver nanoparticles. Inorg Chem Commun 146:110176
Aravinthan A, Govarthanan M, Selvam K et al (2015) Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects. Int J Nanomedicine 10:1977–1983. https://doi.org/10.2147/IJN.S79106
Arumai Selvan D, Mahendiran D, Senthil Kumar R, Kalilur Rahiman A (2018) Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: phytochemical, antioxidant and in vitro cytotoxicity studies. J Photochem Photobiol, B 180:243–252. https://doi.org/10.1016/j.jphotobiol.2018.02.014
Ashraf MA, Peng WX, Fakhri A et al (2019) Manganese disulfide-silicon dioxide nano-material: synthesis, characterization, photocatalytic, antioxidant and antimicrobial studies. J Photochem Photobiol B Biol 198:111579. https://doi.org/10.1016/j.jphotobiol.2019.111579
Aygün A, Gülbağça F, Nas MS et al (2020) Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: a novel approach in phytonanotechnology. J Pharmaceut Biomed Anal 179:113012. https://doi.org/10.1016/j.jpba.2019.113012
Babu B, Palanisamy S, Vinosha M et al (2020) Bioengineered gold nanoparticles from marine seaweed Acanthophora spicifera for pharmaceutical uses: antioxidant, antibacterial, and anticancer activities. Bioprocess Biosyst Eng 43:2231–2242. https://doi.org/10.1007/s00449-020-02408-3
Balakrishnan S, Ibrahim KS, Duraisamy S et al (2020) Antiquorum sensing and antibiofilm potential of biosynthesized silver nanoparticles of Myristica fragrans seed extract against MDR Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers and typhoid patients. Environ Sci Pollut Res 27:2844–2856. https://doi.org/10.1007/s11356-019-07169-5
Behzad F, Naghib SM, Kouhbanani MAJ et al (2021) An overview of the plant-mediated green synthesis of noble metal nanoparticles for antibacterial applications. J Ind Eng Chem 94:92–104. https://doi.org/10.1016/j.jiec.2020.12.005
Bin-Jumah M, AL-Abdan M, Albasher G, Alarifi S (2020) Effects of green silver nanoparticles on apoptosis and oxidative stress in normal and cancerous human hepatic cells in vitro. IJN 15:1537–1548. https://doi.org/10.2147/IJN.S239861
Borah D, Das N, Sarmah P, Ghosh K, Chandel M, Rout J, Pandey P, Ghosh NN, Bhattacharjee CR (2023) A facile green synthesis route to silver nanoparticles using cyanobacterium Nostoc carneum and its photocatalytic, antibacterial and anticoagulative activity. Mater Today Commun 34:105110
Chiarathanakrit C, Mayakun J, Prathep A, Kaewtatip K (2019) Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays. Int J Biol Macromol 121:71–76. https://doi.org/10.1016/j.ijbiomac.2018.09.191
Chinnappan S, Kandasamy S, Arumugam S et al (2018) Biomimetic synthesis of silver nanoparticles using flower extract of Bauhinia purpurea and its antibacterial activity against clinical pathogens. Environ Sci Pollut Res 25:963–969. https://doi.org/10.1007/s11356-017-0841-1
Cottet-Rousselle C, Ronot X, Leverve X, Mayol J-F (2011) Cytometric assessment of mitochondria using fluorescent probes. Cytometry A 79A:405–425. https://doi.org/10.1002/cyto.a.21061
Dawood S, Ahmad M, Zafar M et al (2022) Biodiesel synthesis from Prunus bokhariensis non-edible seed oil by using green silver oxide nanocatalyst. Chemosphere 291:132780. https://doi.org/10.1016/j.chemosphere.2021.132780
de Jesus RA, de Assis GC, de Oliveira RJ et al (2021) Environmental remediation potentialities of metal and metal oxide nanoparticles: mechanistic biosynthesis, influencing factors, and application standpoint. Environ Technol Innov. https://doi.org/10.1016/j.eti.2021.101851
Devi TA, Sivaraman RM, Thavamani SS, Amaladhas TP, AlSalhi MS, Devanesan S, Kannan MM (2022) Green synthesis of plasmonic nanoparticles using Sargassum ilicifolium and application in photocatalytic degradation of cationic dyes. Environ Res 208:112642
Ebrahimzadeh MA, Naghizadeh A, Amiri O et al (2020) Green and facile synthesis of Ag nanoparticles using Crataegus pentagyna fruit extract (CP-AgNPs) for organic pollution dyes degradation and antibacterial application. Bioorg Chem 94:103425. https://doi.org/10.1016/j.bioorg.2019.103425
Ellouzi I, Bouddouch A, Bakiz B et al (2021) Glucose-assisted ball milling preparation of silver-doped biphasic TiO2 for efficient photodegradation of Rhodamine B: effect of silver-dopant loading. Chem Phys Lett 770:138456. https://doi.org/10.1016/j.cplett.2021.138456
Fageria L, Pareek V, Dilip RV et al (2017) Biosynthesized protein-capped silver nanoparticles induce ROS-dependent proapoptotic signals and prosurvival autophagy in cancer cells. ACS Omega 2:1489–1504. https://doi.org/10.1021/acsomega.7b00045
Gopu M, Kumar P, Selvankumar T et al (2021) Green biomimetic silver nanoparticles utilizing the red algae Amphiroa rigida and its potent antibacterial, cytotoxicity and larvicidal efficiency. Bioprocess Biosyst Eng 44:217–223. https://doi.org/10.1007/s00449-020-02426-1
Govarthanan M, Selvankumar T, Manoharan K et al (2014) Biosynthesis and characterization of silver nanoparticles using panchakavya, an Indian traditional farming formulating agent. IJN 9:1593–1599. https://doi.org/10.2147/IJN.S58932
Govarthanan M, Cho M, Park J-H et al (2016a) Cottonseed oilcake extract mediated green synthesis of silver nanoparticles and its antibacterial and cytotoxic activity. J Nanomater. https://doi.org/10.1155/2016/7412431
Govarthanan M, Seo Y-S, Lee K-J et al (2016b) Low-cost and eco-friendly synthesis of silver nanoparticles using coconut (Cocos nucifera) oil cake extract and its antibacterial activity. Artif Cells Nanomed Biotechnol 44:1878–1882. https://doi.org/10.3109/21691401.2015.1111230
Govindappa M, Tejashree S, Thanuja V et al (2021) Pomegranate fruit fleshy pericarp mediated silver nanoparticles possessing antimicrobial, antibiofilm formation, antioxidant, biocompatibility and anticancer activity. J Drug Delivery Sci Technol 61:102289. https://doi.org/10.1016/j.jddst.2020.102289
Hamedi S, Shojaosadati SA (2019) Rapid and green synthesis of silver nanoparticles using Diospyros lotus extract: evaluation of their biological and catalytic activities. Polyhedron 171:172–180. https://doi.org/10.1016/j.poly.2019.07.010
Harborne AJ (1998) Phytochemical methods a guide to modern techniques of plant analysis, 3rd edn. Springer, Netherlands
Heinemann MG, Rosa CH, Rosa GR, Dias D (2021) Biogenic synthesis of gold and silver nanoparticles used in environmental applications: a review. Trends Environ Anal Chem 30:e00129. https://doi.org/10.1016/j.teac.2021.e00129
Hojjati-Najafabadi A, Salmanpour S, Sen F et al (2022) A tramadol drug electrochemical sensor amplified by biosynthesized Au nanoparticle using Mentha aquatic extract and ionic liquid. Top Catal 65:587–594. https://doi.org/10.1007/s11244-021-01498-x
Hojjati-Najafabadi A, Davar F, Enteshari Z, Hosseini-Koupaei M (2021a) Antibacterial and photocatalytic behaviour of green synthesis of Zn0.95Ag0.05O nanoparticles using herbal medicine extract Ceramics International 47: 31617–31624, doi: https://doi.org/10.1016/j.ceramint.2021.08.042
Kayalvizhi S, Sengottaiyan A, Selvankumar T et al (2020) Eco-friendly cost-effective approach for synthesis of copper oxide nanoparticles for enhanced photocatalytic performance. Optik 202:163507. https://doi.org/10.1016/j.ijleo.2019.163507
Kitherian S, Thangapandi V, Jesu Antony MR (2021) Seaweed Lobophora variegata-based silver nanopesticide for environmental friendly management of economically important pest Spodoptera litura. Environ Nanotechnol Monitor Manage 16:100531. https://doi.org/10.1016/j.enmm.2021.100531
Korbekandi H, Mohseni S, Mardani Jouneghani R et al (2016) Biosynthesis of silver nanoparticles using Saccharomyces cerevisiae. Artif Cells Nanomed Biotechnol 44:235–239. https://doi.org/10.3109/21691401.2014.937870
Kumar P, Senthamilselvi S, Govindaraju M (2014) Phloroglucinol-encapsulated starch biopolymer: preparation, antioxidant and cytotoxic effects on HepG2 liver cancer cell lines. RSC Adv 4:26787. https://doi.org/10.1039/c4ra02621g
Kumar VA, Uchida T, Mizuki T et al (2016) Synthesis of nanoparticles composed of silver and silver chloride for a plasmonic photocatalyst using an extract from a weed Solidago altissima (goldenrod). Adv Nat Sci Nanosci Nanotechnol 7:015002. https://doi.org/10.1088/2043-6262/7/1/015002
Lakhan MN, Chen R, Shar AH et al (2020) Eco-friendly green synthesis of clove buds extract functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity. J Microbiol Methods 173:105934. https://doi.org/10.1016/j.mimet.2020.105934
Lee K-J, Park S-H, Govarthanan M et al (2013) Synthesis of silver nanoparticles using cow milk and their antifungal activity against phytopathogens. Mater Lett 105:128–131. https://doi.org/10.1016/j.matlet.2013.04.076
Li JF, Liu Y-C, Chokkalingam M et al (2020) Phytosynthesis of silver nanoparticles using rhizome extract of Alpinia officinarum and their photocatalytic removal of dye under UV and visible light irradiation. Optik 208:164521. https://doi.org/10.1016/j.ijleo.2020.164521
Massironi A, Morelli A, Grassi L et al (2019) Ulvan as novel reducing and stabilizing agent from renewable algal biomass: application to green synthesis of silver nanoparticles. Carbohyd Polym 203:310–321. https://doi.org/10.1016/j.carbpol.2018.09.066
Muthusamy G, Thangasamy S, Raja M et al (2017) Biosynthesis of silver nanoparticles from Spirulina microalgae and its antibacterial activity. Environ Sci Pollut Res Int 24:19459–19464. https://doi.org/10.1007/s11356-017-9772-0
Mythili R, Selvankumar T, Kamala-Kannan S et al (2018) Utilization of market vegetable waste for silver nanoparticle synthesis and its antibacterial activity. Mater Lett 225:101–104. https://doi.org/10.1016/j.matlet.2018.04.111
Narayanan M, Divya S, Natarajan D et al (2021) Green synthesis of silver nanoparticles from aqueous extract of Ctenolepis garcini L. and assess their possible biological applications. Process Biochem 107:91–99. https://doi.org/10.1016/j.procbio.2021.05.008
Ozlem Saygi K, Usta C (2021) Rosa canina waste seed extract-mediated synthesis of silver nanoparticles and the evaluation of its antimutagenic action in Salmonella typhimurium. Mater Chem Phys 266:124537. https://doi.org/10.1016/j.matchemphys.2021.124537
Paiva-Santos AC, Herdade AM, Guerra C et al (2021) Plant-mediated green synthesis of metal-based nanoparticles for dermopharmaceutical and cosmetic applications. Int J Pharm 597:120311. https://doi.org/10.1016/j.ijpharm.2021.120311
Patel P, Nadar VM, Umapathy D et al (2021) Doxorubicin-conjugated platinum theranostic nanoparticles induce apoptosis via inhibition of a cell survival (PI3K/AKT) signaling pathway in human breast cancer cells. ACS Appl Nano Mater 4:198–210. https://doi.org/10.1021/acsanm.0c02521
Popli D, Anil V, Subramanyam AB et al (2018) Endophyte fungi, Cladosporium species-mediated synthesis of silver nanoparticles possessing in vitro antioxidant, anti-diabetic and anti-Alzheimer activity. Artif Cells Nanomed Biotechnol 46:676–683. https://doi.org/10.1080/21691401.2018.1434188
Puja P, Vinita NM, Devan U et al (2020) Fluorescence microscopy-based analysis of apoptosis induced by platinum nanoparticles against breast cancer cells. Appl Organometallic Chem 34:e5740. https://doi.org/10.1002/aoc.5740
Rabinal MK, Kalasad MN, Praveenkumar K et al (2013) Electrochemical synthesis and optical properties of organically capped silver nanoparticles. J Alloy Compd 562:43–47. https://doi.org/10.1016/j.jallcom.2013.01.043
Rajkuberan C, Sudha K, Sathishkumar G, Sivaramakrishnan S (2015) Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L. Spectrochim Acta Part A Mol Biomol Spectrosc 136:924–930. https://doi.org/10.1016/j.saa.2014.09.115
Rajkumar R, Ezhumalai G, Gnanadesigan M (2021) A green approach for the synthesis of silver nanoparticles by Chlorella vulgaris and its application in photocatalytic dye degradation activity. Environm Technol Innov 21:101282. https://doi.org/10.1016/j.eti.2020.101282
Ramkumar VS, Pugazhendhi A, Gopalakrishnan K et al (2017) Biofabrication and characterization of silver nanoparticles using aqueous extract of seaweed Enteromorpha compressa and its biomedical properties. Biotechnol Rep 14:1–7. https://doi.org/10.1016/j.btre.2017.02.001
Sampath G, Govarthanan M, Rameshkumar N et al (2021) Eco-friendly biosynthesis metallic silver nanoparticles using Aegle marmelos (Indian bael) and its clinical and environmental applications. Appl Nanosci. https://doi.org/10.1007/s13204-021-01883-8
Sana SS, Li H, Zhang Z et al (2021) Recent advances in essential oils-based metal nanoparticles: a review on recent developments and biopharmaceutical applications. J Mol Liquids 333:115951. https://doi.org/10.1016/j.molliq.2021.115951
Seitkalieva MM, Samoylenko DE, Lotsman KA et al (2021) Metal nanoparticles in ionic liquids: synthesis and catalytic applications. Coord Chem Rev 445:213982. https://doi.org/10.1016/j.ccr.2021.213982
Selvam K, Sudhakar C, Govarthanan M et al (2017) Eco-friendly biosynthesis and characterization of silver nanoparticles using Tinospora cordifolia (Thunb.) Miers and evaluate its antibacterial, antioxidant potential. J Rad Res Appl Sci 10:6–12. https://doi.org/10.1016/j.jrras.2016.02.005
Selvaraj P, Neethu E, Rathika P et al (2020) Antibacterial potentials of methanolic extract and silver nanoparticles from marine algae. Biocatal Agric Biotechnol 28:101719. https://doi.org/10.1016/j.bcab.2020.101719
Sengottaiyan A, Mythili R, Selvankumar T et al (2016) Green synthesis of silver nanoparticles using Solanum indicum L. and their antibacterial, splenocyte cytotoxic potentials. Res Chem Intermed 42:3095–3103. https://doi.org/10.1007/s11164-015-2199-7
Sherin L, Sohail A, Amjad U-S et al (2020) Facile green synthesis of silver nanoparticles using Terminalia bellerica kernel extract for catalytic reduction of anthropogenic water pollutants. Colloid and Interface Sci Commun 37:100276. https://doi.org/10.1016/j.colcom.2020.100276
Sowani H, Mohite P, Munot H et al (2016) Green synthesis of gold and silver nanoparticles by an actinomycete Gordonia amicalis HS-11: Mechanistic aspects and biological application. Process Biochem 51:374–383. https://doi.org/10.1016/j.procbio.2015.12.013
Suriyakala G, Sathiyaraj S, Gandhi AD et al (2021) Plumeria pudica Jacq. flower extract - mediated silver nanoparticles: Characterization and evaluation of biomedical applications. Inorg Chem Commun 126:108470. https://doi.org/10.1016/j.inoche.2021.108470
Swathilakshmi AV, Abirami S, Geethamala GV et al (2022) Phytonanofabrication of copper oxide mediated by Albizia amara and its photocatalytic efficacy. Mater Lett 314:131911. https://doi.org/10.1016/j.matlet.2022.131911
Trivedi S, Alshehri MA, Aziz AT et al (2021) Insecticidal, antibacterial and dye adsorbent properties of Sargassum muticum decorated nano-silver particles. S Afr J Bot 139:432–441. https://doi.org/10.1016/j.sajb.2021.03.002
Valarmathi N, Ameen F, Almansob A et al (2020) Utilization of marine seaweed Spyridia filamentosa for silver nanoparticles synthesis and its clinical applications. Mater Lett 263:127244. https://doi.org/10.1016/j.matlet.2019.127244
Wang X, Wang Y, Ying Y (2021) Recent advances in sensing applications of metal nanoparticle/metal–organic framework composites. TrAC Trends Anal Chem. https://doi.org/10.1016/j.trac.2021.116395
Xu Y, Hou Y, Wang Y et al (2019) Sensitive and selective detection of Cu2+ ions based on fluorescent Ag nanoparticles synthesized by R-phycoerythrin from marine algae Porphyra yezoensis. Ecotoxicol Environ Saf 168:356–362. https://doi.org/10.1016/j.ecoenv.2018.10.102
Yılmaz Öztürk B, Yenice Gürsu B, Dağ İ (2020) Antibiofilm and antimicrobial activities of green synthesized silver nanoparticles using marine red algae Gelidium corneum. Process Biochem 89:208–219. https://doi.org/10.1016/j.procbio.2019.10.027
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Lavanya, G., Anandaraj, K., Gopu, M. et al. Green chemistry approach for silver nanoparticles synthesis from Halimeda macroloba and their potential medical and environmental applications. Appl Nanosci 13, 5865–5875 (2023). https://doi.org/10.1007/s13204-023-02812-7
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DOI: https://doi.org/10.1007/s13204-023-02812-7