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Characterization and evaluation of cytotoxic effect, antioxidant and antimicrobial activities of zinc oxide nanoparticles derived from Justicia adhatoda

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Abstract

The current study describes a green synthesis method for preparing zinc oxide nanoparticles (ZnO-NPs) using Justicia adhatoda to investigate the different pathogen and toxic assays. Synthesized nanoparticles are hexagonal and 40 nm in size. The antifungal activity of ZnO-NPs was analyzed against three strains: Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. The antimicrobial activity of ZnO-NPs as tested against Bacillus, Escherichia coli, and Staphylococcus aureus. The ZnO-NPs produced exhibited significant free radical scavenging effects of 200 µg/mL-73.12%. ZnO-NPs were evaluated against MCF-7 human breast cancer cells at an LD50 concentration of 50 µg/mL. The results showed that biosynthesized ZnO-NPs have efficient inhibitory activity against fungal and bacterial strains and cytotoxicity against human MCF-7 breast cancer cell lines.

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Authors and Affiliations

  1. Nanobiomedicine Laboratory, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai, 600077, India

    S. Rajeshkumar & M. Tharani

  2. Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Science (SIMATS), Chennai, TN, 602105, India

    J. Santhoshkumar

  3. Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603110, India

    P. Senthil Kumar

  4. Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603110, India

    P. Senthil Kumar

  5. Department of Sustainable Engineering, Institute of Biotechnology, Saveetha School of Engineering, SIMATS, Chennai, 602105, India

    A. Saravanan

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  1. S. Rajeshkumar
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  2. J. Santhoshkumar
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Correspondence to P. Senthil Kumar.

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Rajeshkumar, S., Santhoshkumar, J., Kumar, P.S. et al. Characterization and evaluation of cytotoxic effect, antioxidant and antimicrobial activities of zinc oxide nanoparticles derived from Justicia adhatoda. Appl Nanosci 13, 3993–4004 (2023). https://doi.org/10.1007/s13204-022-02670-9

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