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Durotaxis behavior of bEnd.3 cells on soft substrate with patterned platinum nanoparticle array

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Abstract

The directional arrangement of cells has crucial effect in tissue engineering fields such as wound healing and scar repair. Studies have shown that continuous nanostructures have directional regulatory effect on cells, but whether discontinuous nanostructures have the same regulatory effect on cells is also worthy of further study. Here, a series of discontinuous platinum nanoparticles (PtNPs) patterned on the surface of PDMS (PtNPs-PDMS&Glass) and glass (PtNPs-Glass) substrates were developed to investigate the effect on bEnd.3 cell durotaxis. The laser interference lithography and nanotransfer printing method were employed to fabricate the substrates. It was found that about 80% cells orderly arranged on the PtNPs-PDMS&Glass substrate, but only 20% cells orderly arrangement on the PtNPs-Glass substrate, and the number of cells on the PtNPs-PDMS&Glass substrate was five times more than that on the PDMS coated glass substrate (PDMS&Glass). The results suggested that patterning PtNPs on the PDMS substrate not only provided the topographical guidance for cells just like continuous nanostructures, but also promoted cell adhesion and growth. In addition, an improved whole cell coupling model was used to investigate and explain the cell durotaxis from the perspective of mechanism. These findings show the possibility of discontinuous nanostructures in regulating cell arrangement, and offer a useful method for the design of biological functional substrate, as well as help to understand the mechanism of cell durotaxis.

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Abbreviations

PDMS:

Polydimethylsiloxane

PtNPs:

Platinum nanoparticles

PDMS&Glass:

PDMS coated glass

PtNPs-PDMS&Glass:

Platinum nanoparticles patterned on the PDMS coated glass

PtNPs-Glass:

Platinum nanoparticles patterned on the surface of glass

DMEM:

Dulbecco’s modified Eagle medium

FBS:

Fetal bovine serum

PBS:

Phosphate buffered saline

DAPI:

4′,6-Diamidino-2-phenylindole

PMMA:

Poly(methyl methacrylate)

SEM:

Scanning electron microscopy

AFM:

Atomic force microscope

FM:

Fluorescence microscopy

ECM:

Extracellular matrix

bEnd.3:

Mouse brain microvascular endothelial cells

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Acknowledgements

This work was supported by National Key R&D Program of China (No.2017YFE0112100), EU H2020 Program (MNR4SCELL No.734174), Jilin Provincial Science and Technology Program (Nos.20180414002GH, 20180414081GH, 20180520203JH, 20190702002GH, 20190201287JC and 20200901011SF), and “111” Project of China (D17017).

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Authors and Affiliations

  1. International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun, 130022, China

    Xiaomin Wu, Li Li, Zecheng Lei, Fan Yang, Ri Liu, Lu Wang & Zuobin Wang

  2. Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun, 130022, China

    Xiaomin Wu, Li Li, Zecheng Lei, Fan Yang, Ri Liu, Lu Wang & Zuobin Wang

  3. Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, Tianjin University, Tianjin, 300350, China

    Xinyao Zhu

  4. JR3CN & IRAC, University of Bedfordshire, Luton, LU1 3JU, UK

    Zuobin Wang

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  1. Xiaomin Wu
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  2. Li Li
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  3. Zecheng Lei
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  4. Fan Yang
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  5. Ri Liu
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  6. Lu Wang
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  8. Zuobin Wang
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Correspondence to Li Li or Zuobin Wang.

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Wu, X., Li, L., Lei, Z. et al. Durotaxis behavior of bEnd.3 cells on soft substrate with patterned platinum nanoparticle array. Appl Nanosci 11, 611–620 (2021). https://doi.org/10.1007/s13204-020-01618-1

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  • DOI: https://doi.org/10.1007/s13204-020-01618-1

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