Abstract
Herein, we prepared dopamine assistant-synthesized nanoflowers (dNFs) and norepinephrine assistant-synthesized nanoflowers (neNFs) as novel nano-biocatalysts with intrinsic and effective peroxidase mimic activities. The NFs dispersed in solution or immobilized on the filter membrane served as the Fenton reagent in the presence of hydrogen peroxide (H2O2) allowing spectrophotometric and colorimetric detection of m-cresol and dopamine. Due to its high polar surface property and porosity, the neNFs exhibited remarkably high catalytic activity than dNFs in both solution or in immobilized states. The activity performance of neNFs as a function of varied concentrations of neNFs or m-cresol or dopamine or H2O2; and reaction time was also studied. The oxidative reaction of 0.5 mM m-cresol (in the presence of 4-AAP and H2O2) or 0.25 mM dopamine (in the presence of H2O2) catalyzed by the neNFs (1 mg/mL) were visually detected within 10 min in solution. Whereas, after immobilization on filter membrane, the colorimetric detection of the same concentrations of m-cresol or dopamine was achieved in 30 min using neNFs (2 mg/mL). Furthermore, the effect of repeated use of the neNFs in solution or immobilized states on the catalytic activity was also investigated. We expect that, the NFs formed from non-enzyme molecules possess peroxidase mimic activities that could imply to a study for bioanalytical and biomedical application in the near future.
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We appreciate Erciyes University Technology Research and Implementation Center for assistance with SEM operation. This work was supported by a grant from the Erciyes University Scientific Research Office (FCD-2018-8242).
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SD performed all experiments as a first author. CC contributed to experiments IO and AKM conceived the original idea and designed the project. SD, CC, AKM and IO mainly wrote the manuscript.
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Dadi, S., Celik, C., Mandal, A.K. et al. Dopamine and norepinephrine assistant-synthesized nanoflowers immobilized membrane with peroxidase mimic activity for efficient detection of model substrates . Appl Nanosci 11, 117–125 (2021). https://doi.org/10.1007/s13204-020-01577-7
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DOI: https://doi.org/10.1007/s13204-020-01577-7