Abstract
Objective
Although organic–inorganic hybrid nanoflowers (hNFs) with much enhanced catalytic activity and stability were fabricated using proteins and enzymes, in this study, for the first time, we report synthesis of allicin and copper ion (Cu2+) coordinated NFs and investigate their peroxidase-like and antimicrobial activities.
Results
The allicin (active ingredient of Allium sativum) and Cu2+ was acted as an organic and inorganic part, respectively for synthesis of the Cu-hNFs. The hNFs were characterized by various techniques. Spherical, uniform, mono-dispersed and flower-like-shaped morphology of the hNFs (synthesized at pH 5) were imaged by scanning electron microscopy. The presence of Cu metal in the hNFs was detected by energy dispersive X-ray spectroscopy. Characteristic bonds stretching and bending for structural analysis of the hNFs were carried out by Fourier transform infrared spectrometry. In terms of applications, the hNFs showed quite effective peroxidase-like activity towards to guaiacol (used as a model substrate) in the presence of hydrogen peroxide (H2O2) through Fenton reaction. We demonstrated that the NFs exhibited ~ 200% and ~ 500% higher catalytic activities in 1 h (hr) and 3 h (hrs) than their initial catalytic activity measured in 5 minute (min). Additionally, effective antibacterial properties of the Cu-hNFs were observed against fish pathogen bacteria (Aeromonas hydrophila, Vibrio parahaemolyticus, and Lactococcus garvieae).
Conclusions
We finally demonsrated that allicin based hybrid nanomaterial can be prepared by a relatively cheap, one step, easy and eco-friendly method. The allicin hNFs can be considered as novel Fenton agent for peroxidase like activity and bactericidal.
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References
Akhtar MS, Panwar J, Yun Y (2013) Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustain Chem Eng 1(6):591–602. https://doi.org/10.1021/sc300118u
Altinkaynak C, Yilmaz I, Koksal Z, Özdemir H, Ocsoy I, Özdemir N (2016) Preparation of lactoperoxidase incorporated hybrid nanoflower and its excellent activity and stability. Int J Biol Macromol 84:402–409. https://doi.org/10.1016/j.ijbiomac.2015.12.018
Altinkaynak C, Tavlasoglu S, Kalin R, Sadeghian N, Ozdemir H, Ocsoy I, Özdemir N (2017) A hierarchical assembly of flower-like hybrid Turkish black radish peroxidase-Cu2+ nanobiocatalyst and its effective use in dye decolorization. Chemosphere 182:122–128. https://doi.org/10.1016/j.chemosphere.2017.05.012
Baldemir A, Köse NB, Ildiz N, Ilgun S, Yusufbeyoglu S, Yilmaz V, Ocsoy I (2017) Synthesis and characterization of green tea (Camellia sinensis (L.) Kuntze) extract and its majör components-based nanoflowers: a new strategy to enhance antimicrobial activity. RSC Adv 7:44303. https://doi.org/10.1039/c7ra07618e
Borah D, Hazarika M, Tailor P, Silva AR, Chetia B, Singaravelu G, Das P (2018) Starch-templated bio-synthesis of gold nanoflowers for in vitro antimicrobial and anticancer activities. Appl Nanosci 8:241–253. https://doi.org/10.1007/s13204-018-0793-x
Celik C, Ildiz N, Ocsoy I (2020) Building block and rapid synthesis of catecholamines-inorganic nanoflowers with their peroxidase-mimicking and antimicrobial activities. Sci Rep 10:2903. https://doi.org/10.1038/s41598-020-59699-5
Cho IS, Kim DW, Lee S, Kwak CH, Bae ST, Noh JH, Yoon SH, Jung HS, Kim DW, Hong KS (2008) Synthesis of Cu2PO4OH hierarchical superstructures with photocatalytic activity in visible light. Adv Funct Mater 18:2154–2162. https://doi.org/10.1002/adfm.200800167
Ge J, Lei J, Zare RN (2012) Protein-inorganic hybrid nanoflowers. Nature Nanotech 7:428–432. https://doi.org/10.1038/nnano.2012.80
Ildiz N, Baldemir A, Altinkaynak C, Özdemir N, Yilmaz V, Ocsoy I (2017) Self assembled snowball-like hybrid nanostructures comprising Viburnum opulus L. extract and metal ions for antimicrobial and catalytic applications. Enzyme Microb Technol 102:60–66. https://doi.org/10.1016/j.enzmictec.2017.04.003
Kizildag N, Cenkseven Ş, Koca FD, Sagliker HA, Darici C (2019) How titanium dioxide and zinc oxide nanoparticles do affect soil microorganism activity? Eur J Soil Biol 91:18–24. https://doi.org/10.1016/j.ejsobi.2019.01.001
Lee HR, Chung M, Kim MI, Ha SH (2017) Preparation of glutaraldehyde-treated lipase-inorganic hybrid nanoflowers and their catalytic performance as immobilized enzymes. Enzyme Microb Technol 105:24–29. https://doi.org/10.1016/j.enzmictec.2017.06.006
Leng Y, Fu L, Ye L, Li B, Xu X, Xing X, He J, Song Y, Leng C, Guo Y, Ji X, Lu Z (2016) Protein-directed synthesis of highly monodispersed, spherical gold nanoparticles and their applications in multidimensional sensing. Sci Rep 6:28900. https://doi.org/10.1038/srep28900
Li L, Weng J (2010) Enzymatic synthesis of gold nanoflowers with trypsin. Nanotechnology 21(30):305603. https://doi.org/10.1088/0957-4484/21/30/305603
Lin Z, Xiao Y, Yin Y, Hu W, Liu W, Yang H (2014) Facile synthesis of enzyme-inorganic hybrid nanoflowers and its application as a colorimetric platform for visual detection of hydrogen peroxide and phenol. ACS Appl Mater Interfaces 6:10775–10782. https://doi.org/10.1021/am502757e
Li H, Hou J, Duan L, Ji C, Zhang Y, Chen V (2017) Graphene oxide-enzyme hybrid nanoflowers for efficient water soluble dye removal. J Hazard Mater 338:93–101. https://doi.org/10.1016/j.jhazmat.2017.05.014
Li L, Niu R, Zhang Y (2018) Ag-Au bimetallic nanocomposites stabilized with organic-inorganic hybrid microgels: synthesis and their regulated optical and catalytic properties. RSC Adv 8:12428–12438. https://doi.org/10.1039/c8ra01343h
Ma N, Sargent EH, Kelley SO (2009) One-step DNA-programmed growth of luminescent and biofunctionalized nanocrystals. Nature Nanotech 4:121–125. https://doi.org/10.1038/NNANO.2008.373
Mittal AK, Chisti Y, Banerjee UC (2013) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31(2):346–356. https://doi.org/10.1016/j.biotechadv.2013.01.003
Nhung TT, Bu Y, Lee SW (2013) Facile synthesis of chitosan-mediated gold nanoflowers as surface-enhanced Raman scattering (SERS) substrates. J Cryst Growth 373:132–137. https://doi.org/10.1016/j.jcrysgro.2012.09.042
Ocsoy I, Gulbakan B, Chen T, Zhu G, Chen Z, Sari MM, Peng L, Xiong X, Fang X, Tan W (2013) DNA-guided metal-nanoparticle formation on graphene oxide surface. Adv Mater 25(16):2319–2325. https://doi.org/10.1002/adma.201204944
Ocsoy I, Dogru E, Usta S (2015) A new generation of flowerlike horseradish peroxides as a nanobiocatalyst for superior enzymatic activity. Enzyme Microb Technol 75–76:25–29. https://doi.org/10.1016/j.enzmictec.2015.04.010
Shende P, Kasture P, Gaud RS (2018) Nanoflowers: the future trend of nanotechnology for multi-applications. Artif Cells Nanomed Biotechnol 46:413–422. https://doi.org/10.1080/21691401.2018.1428812
Sreedharan SM, Singh R (2019) Ciprofloxacin functionalized biogenic gold nanoflowers as nanoantibiotics against pathogenic bacterial strains. Int J Nanomed 14:9905–9916. https://doi.org/10.2147/IJN.S224488
Temel S, Gökmen FO, Yaman E (2020) Antibacterial activity of ZnO nanoflowers deposited on biodegradable acrylic acid hydrogel by chemical bath deposition. Bull Mater Sci 43:18. https://doi.org/10.1007/s12034-019-1967-1
Wang LB, Wang YC, He R, Zhuang A, Wang X, Zeng J, Hou JG (2013) A new nanobiocatalytic system based on allosteric effect with dramatically enhanced enzymatic performance. J Am Chem Soc 135:1272–1275. https://doi.org/10.1021/ja3120136
Weng X, Guo M, Luo F, Chen Z (2017) One-step green synthesis of bimetallic Fe/Ni nanoparticles by eucalyptus leaf extract: Biomolecules identification, characterization and catalytic activity. Chem Eng J 308:904–911. https://doi.org/10.1016/j.cej.2016.09.134
Wu ZF, Wang Z, Zhang Y, Ma YL, He CY, Li H, Chen L, Huo QS, Wang L, Li ZQ (2016) Amino acids-incorporated nanoflowers with an intrinsic peroxidase-like activity. Sci Rep 6:22412. https://doi.org/10.1038/srep22412
Ye R, Zhu C, Song Y, Lu Q, Ge X, Yang X, Zhu MJ, Du D, Li H, Lin Y (2016) Bioinspired synthesis of all-in-one organic-inorganic hybrid nanoflowers combined with a handheld pH meter for on-site detection of food pathogen. Small 12:3094–3100. https://doi.org/10.1002/smll.201600273
Zhang M, Zhao Y, Yan L, Peltier R, Hui W, Yao X, Cui Y, Chen X, Sun H, Wang Z (2016) Interfacial engineering of bimetallic Ag/Pt nanoparticles on reduced graphene oxide matrix for enhanced antimicrobial activity. ACS Appl Mater Interfaces 8:8834–8840. https://doi.org/10.1021/acsami.6b01396
Zhu L, Gong L, Zhang Y, Wang R, Ge J, Liu Z, Zare RN (2013) Rapid detection of phenol using a membrane containing laccase nanoflowers. Chem Asian J 8:2358–2360. https://doi.org/10.1002/asia.201300020
Zhu X, Huang J, Liu J, Zhang H, Jiang J, Yu R (2017) Dual enzyme-inorganic hybrid nanoflowers incorporated microfluidic paper-based analytic devices (µPADs) biosensor for sensitive visualized detection of glucose. Nanoscale 9:5658–5663. https://doi.org/10.1039/c7nr00958e
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This work was supported by a grant from the Erciyes University Scientific Research Office (THD-2018-8069).
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Koca, F.D., Demirezen Yilmaz, D., Ertas Onmaz, N. et al. Green synthesis of allicin based hybrid nanoflowers with evaluation of their catalytic and antimicrobial activities. Biotechnol Lett 42, 1683–1690 (2020). https://doi.org/10.1007/s10529-020-02877-2
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DOI: https://doi.org/10.1007/s10529-020-02877-2