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Simple fabrication of sericin/graphene nanocomposites for application in articular cartilage repair in knee joints in nursing care

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Abstract

In the present work, sericin/reduced graphene oxide (SSG) nanocomposites are synthesized through a low cost, facile and biological approach. An anticipated amount of sericin powder was mixed to GO dispersion with sericin/GO ratios of 10:1, 50:1, and 100:1 to obtain sericin/rGO nanocomposites such as SSG10, SSG50, and SSG100, respectively. TEM images revealed the rough surface of graphene sheets with obtained proteins assembled on the surface of the GO nanosheets. Further, biological studies revealed that SSG nanocomposites significantly promoted the collagen levels and glycosaminoglycan. Cell proliferation results showed the effective growth of MSCs with SSG100 than GO, SSG10, and SSG50. Also, the confocal microscopic images exhibited the spindle-like morphology of MSCs with uniform size and increased cell density on the 5th day of cell proliferation in case of SSG100 than GO and other nanocomposites. Therefore, the present results suggest that the fabricated SSG hybrid material could act as promising candidates for future applications of repairing articular cartilage in knee joints in nursing care.

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Author notes

  1. Yanrong Yuan and Haizhen Liu contributed equally to this work.

Authors and Affiliations

  1. Department of Foot and Ankle Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China

    Yanrong Yuan, Feiyan Liu & Guofeng Guan

  2. Nursing Department, Shi Kou Central Hospital, Dongying, Shandong, China

    Haizhen Liu

  3. The Third Department of Psychiatry, Binzhou People’s Hospital, Binzhou, China

    Na Zheng

  4. Department of Reproductive Medicine, Binzhou Medical University Hospital, Binzhou, China

    Linggong Gao & Guilan Zhang

  5. Department of Hand (Micro) Surgery, Binzhou Medical University Hospital, Binzhou, China

    Guilan Zhang

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  1. Yanrong Yuan
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  2. Haizhen Liu
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  3. Na Zheng
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  4. Linggong Gao
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  5. Feiyan Liu
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  6. Guofeng Guan
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  7. Guilan Zhang
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Correspondence to Guilan Zhang.

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Yuan, Y., Liu, H., Zheng, N. et al. Simple fabrication of sericin/graphene nanocomposites for application in articular cartilage repair in knee joints in nursing care. Appl Nanosci 10, 695–702 (2020). https://doi.org/10.1007/s13204-019-01150-x

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  • DOI: https://doi.org/10.1007/s13204-019-01150-x

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