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Co-immobilization of multiple enzymes onto surface-functionalized magnetic nanoparticle for the simultaneous hydrolysis of multiple substrates containing industrial wastes

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Abstract

The present study focused on the co-immobilization of multi-enzymes onto magnetic nanoparticles (MNPs) and surface-functionalized MNPs for the hydrolysis of fish processing solid waste (FPSW) containing complex molecules. The MNPs were synthesized and surface-functionalized by chemical (cMNP and SF-cMNP) and biological method [biologically synthesized iron oxide magnetic nanoparticle (bIOMNP)] and was characterized by various instrumental analyses. Lipase and protease produced from Streptomyces thermolineatus were co-immobilized onto the synthesized bare and surface-functionalized MNPs. At the optimum conditions, the lipase and protease loading capacity of cMNP were found as 1000 and 783 U/g, respectively, whereas the lipase and protease loading capacity of SF-cMNP were 2920 and 2350 U/g, respectively, and bIOMNP was 2800 and 2196 U/g, respectively. The thermal, pH and storage stabilities of the co-immobilized enzymes were greatly enhanced compared to the free enzymes and the Vmax and Km of the free and co-immobilized enzymes were also determined. The free and the co-immobilized enzymes were used for the hydrolysis of lipids and proteins in FPSW. The percentage hydrolysis of lipids and proteins in FPSW was 52.8 and 61.8%, respectively, for LP-cMNP, 76.2 and 84.1%, respectively, for LP-SF-cMNP and 73.9 and 82.1%, respectively, for LP-bIOMNP.

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Acknowledgements

The authors are grateful to the Department of Biotechnology, Nanotechnology research centre, Department of Physics & Nanotechnology, SRM Institute of Science and Technology and SAIF, IIT-Madras for extending the analytical facilities.

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Authors and Affiliations

  1. Biomolecules and Biocatalysis Laboratory, Department of Biotechnology, SRM Institute of Science and Technology, Kancheepuram District, Kattankulathur, Tamil Nadu, 603203, India

    S. Hepziba Suganthi, K. V. Swathi, Raagini Biswas, Sneha Basker & K. Ramani

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  1. S. Hepziba Suganthi
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  2. K. V. Swathi
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  3. Raagini Biswas
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  4. Sneha Basker
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  5. K. Ramani
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Correspondence to K. Ramani.

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Hepziba Suganthi, S., Swathi, K.V., Biswas, R. et al. Co-immobilization of multiple enzymes onto surface-functionalized magnetic nanoparticle for the simultaneous hydrolysis of multiple substrates containing industrial wastes. Appl Nanosci 9, 1439–1457 (2019). https://doi.org/10.1007/s13204-019-01125-y

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