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The ectomycorrhizae of Lactarius rimosellus and Lactarius acatlanensis with the endangered Fagus grandifolia var. mexicana

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Abstract

In the mountains of Veracruz State (east of Mexico), the southernmost limit of Fagus in the Americas, two relict forests of F. grandifolia var. mexicana were visited between 2007 and 2009 and 2013–2014, in order to study the ectomycorrhizal (EcM) systems of these trees with Lactarius rimosellus (L. subg. Russularia) and with L. acatlanensis (L. subg. Lactarius). We confirmed their EcM association through comparison of the internal transcribed spacer (ITS) of nuclear ribosomal DNA sequences from the mycobiont in the ectomycorrhizal systems and basidiomes sampled in the forest stands. The phytobiont in the ectomycorrhizae was also identified through the comparison of ITS sequences from EcM root tips and leaves of F. grandifolia var. mexicana. Detailed descriptions on the morphology and anatomy of EcM systems and microscopical features of mantle layers and Hartig net are presented and illustrated.

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Acknowledgements

We appreciate the assistance in the field and in the laboratory to Biols. D. Ramos and J.C. Corona (both at Instituto de Ecología, A.C.). We recognize the support given by CONACYT (CB 169172) to study the EcM fungi associated to Fagus grandifolia var. mexicana in the montane cloud forest of Central Veracruz and by the postdoctoral grant to A. Ramos. We recognize the support given by CONACYT (225382) to the Laboratorio de Presecuenciación, Red Biodiversidad y Sistemática, INECOL. Ing. Bertha Pérez at this lab aid in some molecular procedures.

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  1. Instituto de Ecología, Red Biodiversidad y Sistemática, A.C., P.O. Box 63, Xalapa, 91000, Veracruz, Mexico

    Leticia Montoya, Victor M. Bandala, Antero Ramos & Edith Garay-Serrano

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  1. Leticia Montoya
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  2. Victor M. Bandala
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  3. Antero Ramos
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Correspondence to Victor M. Bandala.

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Montoya, L., Bandala, V.M., Ramos, A. et al. The ectomycorrhizae of Lactarius rimosellus and Lactarius acatlanensis with the endangered Fagus grandifolia var. mexicana . Symbiosis 73, 135–144 (2017). https://doi.org/10.1007/s13199-017-0489-0

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