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Comparative proteomic analysis of leaf tissue from tomato plants colonized with Rhizophagus irregularis

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Abstract

A comparative proteomic approach was performed to analyze the differential accumulation of leaf proteins in response to the symbiosis between Solanum lycopersicum and the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis. Protein profiling was examined in leaves from tomato plants colonized with AMF (M), as well as non-colonized plants fertilized with low phosphate (20 μM P; NM-LP) and non-colonized plants fertilized with regular phosphate Hoagland’s solution (200 μM P; NM-RP). Comparisons were made between these groups, and 2D-SDS-PAGE revealed that 27 spots were differentially accumulated in M vs. NM-LP. Twenty-three out of the 27 spots were successfully identified by mass spectrometry. Two of these proteins, 2-methylene-furan-3-one reductase and auxin-binding protein ABP19a, were up-accumulated in M plants. The down-accumulated proteins in M plants were associated mainly with photosynthesis, redox, and other molecular functions. Superoxide dismutase, harpin binding protein, and thioredoxin peroxidase were down-accumulated in leaves of M tomato plants when compared to NM-LP and NM-RP, indicating that these proteins are responsive to AMF colonization independently of the phosphate regime under which they were grown. 14-3-3 protein was up-accumulated in NM-RP vs. NM-LP plants, whereas it was down-accumulated in M vs. NM-LP and M vs. NM-RP, regardless of their phosphate nutrition. This suggests a possible regulation by P nutrition and AMF colonization. Our results demonstrate AMF-induced systemic changes in the expression of tomato leaf proteins, including the down-accumulation of proteins related to photosynthesis and redox function.

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Acknowledgments

The authors would like to thank Alicia Chagolla of the Proteomics service lab at CINVESTAV-Irapuato, Gto., Mexico. P-GLI would like to thank CONACYT-Mexico for the scholarship support. This project was funded by SIP-IPN (20131537), CECYT-SINALOA (2007, 2008 and 2009), and CONACYT CB-2008-01-102237. The authors thank Dr. Brandon Loveall of Improvence for English proofreading of the manuscript.

Author contributions

LIPG participated in the experimental design, conducted most of the experimental work (including 2D-electrophoresis and peptide sequence analyses) and drafted the manuscript. JALV participated in image analysis of the two-dimensional gel using the PD-Quest software, and in critical revision of the manuscript. IEMM, HGF and SCL participated in the experimental work and in critical revision of the manuscript. MLM and SMG conceived the study and participated in its design and coordination, as well as drafting of the manuscript. All authors have read and approved the final manuscript.

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Authors and Affiliations

  1. Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Sinaloa, Guasave, Sinaloa, Mexico

    M. López-Meyer, I. E. Maldonado-Mendoza, H. Galindo-Flores & S. Medina-Godoy

  2. PRNDB-FCQB de la Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, Mexico

    L. I. Peinado-Guevara, J. A. López-Valenzuela & S. Medina-Godoy

  3. Escuela de Biología de la Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, Mexico

    L. I. Peinado-Guevara & S. Campista-León

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  1. L. I. Peinado-Guevara
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  2. M. López-Meyer
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  3. J. A. López-Valenzuela
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  6. S. Campista-León
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  7. S. Medina-Godoy
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Correspondence to S. Medina-Godoy.

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Peinado-Guevara, L.I., López-Meyer, M., López-Valenzuela, J.A. et al. Comparative proteomic analysis of leaf tissue from tomato plants colonized with Rhizophagus irregularis . Symbiosis 73, 93–106 (2017). https://doi.org/10.1007/s13199-016-0470-3

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  • DOI: https://doi.org/10.1007/s13199-016-0470-3

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