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Phytometabolomic fingerprinting of selected actinorhizal fruits popularly consumed in North-East India

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Abstract

The biodiversity hub at north-eastern region of India contains actinorhizal plants like Elaeagnus pyriformis, Myrica nagi and Myrica esculenta which serve as functional food for the natives. Apart from having immense nutritional value, fruits of these plants have been utilized in traditional medicine and have displayed antioxidant behavior as observed from our previous findings. Therefore, the aim of the present study was to investigate the phytochemical fingerprints of the fruit juices of some actinorhizal plants using GC-MS method and correlate the data with the therapeutic value of the fresh fruit juice. The results established the abundance of several chemical species with potential medicinal properties such as anti-diabetic, anti-cancer, anti-inflammatory, hepatoprotective activities. However, further studies are needed to isolate and identify the specific phyto-compounds involved in managing various diseases.

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References

  • Abou-Elela GM, Abd-Elnaby H, Ibrahim HAH, Okbah MA (2009) Marine natural products and their potential applications as anti-infective agents. World Appl Sci J 7:872–880

    CAS  Google Scholar 

  • Andrade DS, Leal AC, Ramos ALM, de Goes KCGP (2015) Growth of Casuarina cunninghamiana inoculated with arbuscular mycorrhizal fungi and Frankia actinomycetes. Symbiosis 66:65–75. doi:10.1007/s13199-015-0335-1

    Article  CAS  Google Scholar 

  • Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M (2012) Anti-Inflammatory Property of n-Hexadecanoic Acid: Structural Evidence and Kinetic Assessment. Chem Biol Drug Des 80:434–439. doi:10.1111/j.1747-0285.2012.01418.x

    Article  CAS  PubMed  Google Scholar 

  • Asati BS, Yadav DS (2004) Diversity of horticultural crops in North-Eastern region. ENVIS Bull Himalayan Ecol 12:1–11

    Google Scholar 

  • Azeez L, Badiru R (2012) Antioxidant activity and phytochemical contents of some selected Nigerian fruits and vegetables. American J Chem 2:209–213. doi:10.5923/j.chemistry.20120204.04

    Article  CAS  Google Scholar 

  • Bach L, Faure JD (2010) Role of very-long-chain fatty acids in plant development, when chain length does matter. Comptes Rendus Biologies 333:361–370. doi:10.1016/j.crvi.2010.01.014

    Article  CAS  PubMed  Google Scholar 

  • Basistha BC, Sharma NP, Lepcha L, Arrawatia ML, Sen A (2010) Ecology of Hippophae salicifolia D. Don of temperate and sub-alpine forests of North Sikkim Himalayas-a case study. Symbiosis 50:87–95. doi:10.1007/s13199-009-0033-y

  • Flamini G, Bader A, Cioni PL, Katbeh-Bader A, Morelli I (2004) Composition of the essential oil of leaves, galls, and ripe and unripe fruits of Jordanian Pistacia palaestina Boiss. J Agric Food Chem 52:572–576. doi:10.1021/jf034773t

    Article  CAS  PubMed  Google Scholar 

  • Gloess AN, Vietri A, Wieland F, Smrke S, Schönbächler B, Sánchez López JA, Petrozzi S, Bongers S, Koziorowski T, Yeretzian C (2014) Evidence of different flavour formation dynamics by roasting coffee from different origins: On-line analysis with PTR-ToF-MS. Int J Mass Spectrom 365:324–337. doi:10.1016/j.ijms.2014.02.010

    Article  Google Scholar 

  • Gopalakrishnan S, Kalaiarasi T (2013) Determination of biologically active constituents of the fruits of Cucumis sativus Linn. using GC-MS analysis. Int J Biol Pharm Res 4:523–527

    Google Scholar 

  • Gopalakrishnan K, Udayakumar R (2014) GC-MS Analysis of Phytocompounds of Leaf and Stem of Marsilea quadrifolia (L.). Int J Biochem Res & Rev 4:517–526

    Article  Google Scholar 

  • Goyal AK, Mishra T, Bhattacharya M, Kar P, Sen A (2013) Evaluation of phytochemical constituents and antioxidant activity of selected actinorhizal fruits growing in the forests of Northeast India. J Biosci 38:797–803. doi:10.1007/s12038-013-9363-2

    Article  CAS  PubMed  Google Scholar 

  • Hook.F. (1876) Flora of British India 2: 44

  • Hsouna AB, Trigui M, Mansour RB, Jarraya RM, Damak M, Jaoua S (2011) Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat. Int J Food Microbiol 148:66–72. doi:10.1016/j.ijfoodmicro.2011.04.028

    Article  PubMed  Google Scholar 

  • Huang Y, Zhao J, Zhou L, Wang J, Gong Y, Chen X, Guo Z, Wang Q, Jiang W (2010) Antifungal activity of the essential oil of Illicium verum fruit and its main component trans-anethole. Molecules 15:7558–7569. doi:10.3390/molecules15117558

    Article  CAS  PubMed  Google Scholar 

  • Jeeva S, Lyndem FG, Sawian JT, Laloo RC, Mishra BP (2011) Myrica esculenta Buch.-Ham. ex D. Don.-a potential ethnomedicinal species in a subtropical forest of Meghalaya, northeast India. Asian Pac J Trop Biomed 1:174–177

    Article  Google Scholar 

  • Jumare FM, Yakubu SE, Ameh JB (2015) Antibacterial Activity and Identification of Bioactive Compounds in Extracts of Garlic cloves and Lemon juice by GC-MS Analysis. J Biol Agric Healthcare 5:134–149

    Google Scholar 

  • Kala CP (2005) Ethnomedicinal botany of the Apatani in the Eastern Himalayan region of India. J Ethnobiol Ethnomed 1:1–8. doi:10.1186/1746-4269-1-11

    Article  Google Scholar 

  • Kalaiarasan A, Kumar P, John SA (2011) GC/MS determination of bioactive components of Bulbophyllum kaitense Reichib Leaves Estern Ghats in India. New York Sci J 4:46–49

    Google Scholar 

  • Kar P, Goyal AK, Das AP, Sen A (2014) Antioxidant and pharmaceutical potential of Clerodendrum L.: An overview. Int J Green Pharma 8:210–216

    Article  CAS  Google Scholar 

  • Khamzina A, Lamers JP, Vlek PL (2009) Nitrogen fixation by Elaeagnus angustifolia in the reclamation of degraded croplands of Central Asia. Tree physiol 1–10. doi:10.1093/treephys/tpp017

  • Lalitharani S, Mohan VR, Regini GS (2010) GC-MS analysis of ethanolic extract of Zanthoxylum rhetsa (roxb.) dc spines. J Herb Med Toxicol 4:191–192

    Google Scholar 

  • Murkovic M, Pichler N (2006) Analysis of 5-hydroxymethylfurfual in coffee, dried fruits and urine. Mol Nutr Food Res 50:842–846. doi:10.1002/mnfr.200500262

    Article  CAS  PubMed  Google Scholar 

  • Osmaston AE (1987) A forest flora for Kumaon. Bishen Singh Mahendra Pal Singh, Dehradun, India

    Google Scholar 

  • Palmgren K, Saarsalmi A, Weber A (1985) Nitrogen fixation and biomass production in some Alder clones. Silva Fenn 19:407–420

    Article  Google Scholar 

  • Panthari P, Kharkwal H, Kharwal H, Joshi DD (2012) Myrica nagi: A review on active constituents, biological and therapeutic effects. Int J Pharm Pharm Sci 4:38–42

    Google Scholar 

  • Parmar C, Kaushal MK (1982) E. umbellata Thunb. in wild fruits of sub-Himalayan region. New Delhi, Kalyani Publishers, pp23–25

  • Permar TA, Fisher RF (1983) Nitrogen fixation and accretion by wax myrtle (Myrica cerifera) in slash pine (Pinus elliottii) plantations. Forest Ecol and Management 5:39–46. doi:10.1016/0378-1127(83)90067-1

    Article  CAS  Google Scholar 

  • Ponnamma SU, Manjunath K (2012) GC-MS analysis of phytocomponents in the methanolic extract of Justicia wynaadensis (Nees) T. Anders. Int J Pharm Bio Sci 3:570–576

    CAS  Google Scholar 

  • Rawat S, Jugran A, Giri L, Bhatt ID, Rawal RS (2010) Assessment of antioxidant properties in fruits of Myrica esculenta: A popular wild edible species in Indian Himalayan Region. Evid Based Complementary Altern Med:1–8. doi:10.1093/ecam/neq055

  • Ribeiro A, Berry AM, Pawlowski K, Santos P (2011) Actinorhizal plants. Funct Plant Biol 38:5–7. doi:10.1071/FPv38n9_FO

    Google Scholar 

  • Sen A, Sur S, Bothra AK, Benson DR, Normand P, Tisa LS (2008) The implication of life style on codon usage patterns and predicted highly expressed genes for three Frankia genomes. Anton Leeuw 93:335–346. doi:10.1007/s10482-007-9211-1

    Article  CAS  Google Scholar 

  • Shakya A, Singh GK, Chatterjee SS, Kumar V (2014) Role of fumaric acid in anti-inflammatory and analgesic activities of a Fumaria indica extracts. J Intercult Ethnopharmacol 3:173–178. doi:10.5455/jice.20140912021115

    Article  PubMed  PubMed Central  Google Scholar 

  • Sharma SD, Kumar K (2006) Temperate fruit diversity status and prospects. In: Kishore DK, Sharma SK, Pramanick KK (eds) Temperate Horticulture: Current Scenario. New India Publishing Agency, New Delhi, pp. 27–34

    Google Scholar 

  • Sharma E, Sharma R, Pradhan M (1998) Ecology of Himalayan alder (Alnus nepalensis D. Don). Proceedings Indian National Science Academy Part B 64:59–78

    CAS  Google Scholar 

  • Simeone MLF, Mikich SB, Côcco LC, Hansel FA, Bianconi GV (2011) Chemical Composition of Essential Oils from Ripe and Unripe Fruits of Piper amalago L. var. medium (Jacq.) Yunck and Piper hispidum Sw. J Essent Oil Res 23:54–58. doi:10.1080/10412905.2011.9700483

    Article  CAS  Google Scholar 

  • Sundarraj S, Thangam R, Sreevani V, Kaveri K, Gunasekaran P, Achiraman S, Kannan S (2012) y-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol 141:803–809

    Article  CAS  PubMed  Google Scholar 

  • Sutha S, Mohan VR, Kumaresan S, Murugan C, Athiperumalsami T (2010) Ethnomedicinal plants used by the tribals of Kalakad Mundanthurai Tiger Reserve (KMTR), Western Ghats, Tamil Nadu for the treatment of rheumatism. Indian J Tradit 9:502–509

    Google Scholar 

  • Thanga KKS, Muthukumarasamy S, Mohan VR (2012) GC-MS determination of bioactive components of Canscora perfoliata Lam. (Gentianaceae). J Appl Pharma Sci 2:210–214. doi:10.7324/JAPS.2012.2837

    Google Scholar 

  • Uma B, Prabhakar K, Rajendran S, Sarayu YL (2009) Studies on GC/MS spectroscopic analysis of some bioactive antimicrobial compounds from Cinnamomum zeylanicum. J Med Plants 8:125–131

    CAS  Google Scholar 

  • Vazquez-Cruz MA, Jimenez-Garcia SN, Torres-Pacheco I, Guzman-Maldonado SH, Guevara-Gonzalez RG, Miranda-Lopez R (2012) Effect of maturity stage and storage on flavor compounds and sensory description of berrycactus (Myrtillocactus geometrizans). J Food Sci 77:366–373. doi:10.1111/j.1750-3841.2012.02621.x

    Article  Google Scholar 

  • Yanthan M, Misra AK (2013) Molecular approach to the classification of medicinally important actinorhizal genus Myrica. Indian J Biotechnol 12:133–136

    Google Scholar 

  • Yanthan M, Biate D, Misra AK (2011) Taxonomic resolution of actinorhizal Myrica species from Meghalaya (India) through nuclear rDNA sequence analyses. Funct Plant Biol 38:738–746. doi:10.1071/FP10248 1445-4408/11/090738

    Article  Google Scholar 

  • Zhao L, Chen J, Su J, Li L, Hu SLB, Chen T (2013) In vitro antioxidant and anti-proliferative activities of 5-hydroxymethylfurfural. J Agric Food Chem 61:10604–10611. doi:10.1021/jf403098y

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was partially funded by Department of Biotechnology, Govt. of West Bengal, India through grant no. 206/Bt(Estd.)/RD-22/2014. AS is grateful to the DBT, for providing CREST Award and help in setting up Bioinformatics Centre, in the Department of Botany, University of North Bengal. PK is thankful to Department of Biotechnology, Govt. of India for providing fellowship. PD acknowledges Indian Council of Medical Research, SRF, Govt. of India for providing fellowship.

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Authors and Affiliations

  1. Molecular Cytogenetics Laboratory, Department of Botany, University of North Bengal, Siliguri, -734013, India

    Pallab Kar & Arnab Sen

  2. Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, -734013, India

    Priyankar Dey & Tapas Kumar Chaudhuri

  3. Molecular Genetics Laboratory, Department of Botany, North Eastern Hill University, Shillong, -793022, India

    Arvind Kumar Misra

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  1. Pallab Kar
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Correspondence to Pallab Kar.

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Presented at the 18th International Meeting on Frankia and Actinorhizal Plants (ACTINO2015), August 24–27, 2015, Montpellier, France

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Kar, P., Dey, P., Misra, A.K. et al. Phytometabolomic fingerprinting of selected actinorhizal fruits popularly consumed in North-East India. Symbiosis 70, 159–168 (2016). https://doi.org/10.1007/s13199-016-0415-x

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