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Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for Brucella Testing in milk

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Abstract

In this study, a simple label-free biosensor for Brucella was constructed, which based on the screen-printed carbon electrode (SPCE) modified by Recombinant protein G/gold nanoparticles/graphene oxide (RpG/Au/GO). The impedance responses of the proposed biosensor were measured by electrochemical AC impedance method in Brucella antigen gradient concentration solutions. The results showed that the linear range of this biosensor was from 1.6 × 102 CFU/mL to 1.6 × 108 CFU/mL with the minimum detection limit of 3.2 × 102 CFU/mL (S/N = 3). Moreover, the biosensor for Brucella detection possessed acceptable reproducibility with a relative standard deviation of 5.15% and acceptable stability with a relative standard deviation of 4.68%. The spiked recovery rate in actual pasteurized milk samples was more than 92%. Therefore, the developed biosensor exhibits excellent prospects in the selective quantification detection of Brucella abortus.

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Abbreviations

SPCE:

Screen-printed carbon electrode

RpG:

Recombinant protein G

Au:

Gold nanoparticles

GO:

Graphene oxide

CV:

Cyclic voltammetry

EIS:

Electrochemical impedance spectroscopy

Rct:

Charge transfer resistance

XRD:

X-ray diffraction

SPR:

Surface Plasmon resonance

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Acknowledgements

Thanks to Professor Yueming Zuo for language help in this article.

Funding

Our work has been supported by several organizations. We are sincerely grateful for the financial support from the Project of Youth Fund for Science and Technology Research in Hebei Universities (Grant number: QN2020194), the Project of Youth Fund for National Natural Science Foundation of China (Grant number: 32001791) and the National Natural Science Foundation of China (Grant number 30871445).

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Authors and Affiliations

  1. College of Electrical Engineering, North China University of Science and Technology, Tangshan, 063210, People’s Republic of China

    Hongshuo Chen, Chuanjin Cui & Wensi Zhang

  2. College of Life Sciences, North China University of Science and Technology, Tangshan, 063210, People’s Republic of China

    Haibin Liu

  3. College of Engineering, Shanxi Agricultural University, Taigu, 030801, People’s Republic of China

    Hongshuo Chen & Yueming Zuo

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  1. Hongshuo Chen
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Correspondence to Hongshuo Chen.

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Chen, H., Liu, H., Cui, C. et al. Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for Brucella Testing in milk. J Food Sci Technol 59, 4653–4662 (2022). https://doi.org/10.1007/s13197-022-05544-8

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  • DOI: https://doi.org/10.1007/s13197-022-05544-8

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