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Low carbohydrate high fat flour: its rheology, bread making, physico-sensory and staling characteristics


A low carbohydrate and high fat (LCHF) flour was developed by combining almond flour, desiccated coconut flour, defatted soya flour, dry gluten powder, psyllium husk and skimmed milk powder. Determination of rheological, bread making, nutritional, and staling characteristics of LCHF flour in comparison with wheat flour (WF) was studied. The results showed that LCHF flour had lower amylograph pasting temperature (31.6 °C), peak viscosity (200 BU), farinograph dough stability (0.8 min), and bread volume (315 ml) compared to WF (61.0 °C; 782 BU; 8.7 min; and 525 ml) respectively. The use of additive mixes such as fungal alpha-amylase, sodium stearoyl-2-lactylate and xanthan gum, improved the volume and texture of the LCHF bread. Scanning electron microscope images showed little or no presence of starch granules in LCHF dough and bread. Differential scanning calorimetry studies indicated that, during storage (1–5 days), the enthalpy for gelatinization of endotherm starch increased (0.71–3.40 j/g) in WF bread, however, in LCHF bread this increase was lesser (0.53 to 2.2 j/g) indicating slower staling rate in LCHF bread. The LCHF bread showed lower carbohydrate (13.7%), in-vitro starch digestibility (17.3%) and staling rate, higher protein (22.51%), fat (11.01%), and medium-chain fatty acids than WF bread (51.9%; 38.2%; 12.57%; 3.78%) respectively. The results showed that the developed product would be beneficial for people suffering from diabetics and obesity.

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Availability of data/material

The data is available upon a reasonable request from corresponding author.



Low carbohydrate high fat


Wheat flour


Almond flour, desiccated coconut flour, defatted soya flour, dry gluten powder, psyllium husk and skimmed milk powder


Additive mix


Fungal alpha-amylase


Sodium stearoyl-2-lactylate


Xanthan gum


Rounds per minute


Brabender unit


In-vitro starch digestibility


Differential scanning calorimetry

T0 :

Onset temperature

Tp :

Peak temperature

Te :

End temperature




  • AACC (2010) Approved methods of the American association of cereal chemists, 10th edn. American association of cereal chemists, St. Paul, Minnesota

    Google Scholar 

  • AOAC (2003) Official methods of analysis, 13th edn. Association of Official Analytical Chemists, Washington

  • Cauvain P, Chamberlain N (1988) The bread improving effect of fungal α-amylase. J of Cereal Sci 8:239–248

    CAS  Article  Google Scholar 

  • Czuchajowska Z, Szczodrak J, Pomeranz Y (1992) Characterization and estimation of barley polysaccharides by near-infrared spectroscopy. I barleys, starches, and beta-d-glucans. Cereal Chem 69:413–418

    CAS  Google Scholar 

  • Dat LQ (2018) Functional properties and influences of coconut flour on texture of dough and cookies. Vietnam J Sci Technol 55:100–107

    Article  Google Scholar 

  • De Roos NM, Schouten EG, Katan MB (2001) Consumption of a solid fat rich in lauric acid results in a more favourable serum lipid profile in healthy men and women than consumption of a solid fat rich in trans-fatty acids. J Nutri 131:242–245

    Article  Google Scholar 

  • Denke MA, Grundy SM (1992) Comparison of effects of lauric acid and palmitic acid on plasma lipids. Am J Clinic Nutri 56:895–898

    CAS  Article  Google Scholar 

  • Dhinda F, Jyothilakshmi A, Prakash J, Dasappa I (2012) Effect of ingredients on rheological, nutritional and quality characteristics of high protein, high fibre and low carbohydrate bread. Food Biopro Technol 5:2998–3006

    CAS  Article  Google Scholar 

  • Dissanayake M, Ramchandran L, Donkor ON, Vasiljevic T (2013) Denaturation of whey proteins as a function of heat, pH and protein concentration. Int Dairy J 31:93–99

    CAS  Article  Google Scholar 

  • Englyst KN, Englyst HN, Hudson GJ, Cole TJ, Cummings JH (1999) Rapidly available glucose in foods: an in-vitro measurement that reflects the glycemic response. Am J of Clinic Nutri 69:448–454

    CAS  Article  Google Scholar 

  • Flemming SE, Sosulski FE (1978) Microscopic evaluation of bread fortified with concentrated plant proteins. Cereal Chem 55:373–382

    Google Scholar 

  • Folashade I, Eniola T, Olayemi A (2019) Nutritive value and acceptability of bread fortified with moringa seed powder. J Saudi Soc Agri Sci 18:195–200

    Google Scholar 

  • Goesaert H, Brijs K, Veraverbekec WS, Courtin CM, Gebruers K, Delcour JA (2005) Wheat flour constituents: how they impact bread quality, and how to impact their functionality. Trends in Food Sci and Technol 16:12–30

    CAS  Article  Google Scholar 

  • Goni I, García-Alonso A, Saura-Calixto F (1997) A starch hydrolysis procedure to estimate glycemic index. Nutri Res 17:427–437

    CAS  Article  Google Scholar 

  • Gopalakrishnan J, Menon R, Padmaja G, Sajeev M, Moorthy S (2011) Nutritional and functional characteristics of protein-fortified pasta from sweet potato. Food and Nutri Sci 2:944–955

    CAS  Google Scholar 

  • Gunathilake KDPP, Yalegama C, Kumara AAN (2009) Use of coconut flours as a source of protein and dietary fiber in wheat bread. Asian Food Agro-Ind 2:382–391

    Google Scholar 

  • Hug-Iten S, Handschin S, Conde-Petit B, Escher F (1999) Changes in starch microstructure on baking and staling of wheat bread. LWT Food Sci and Technol 32:255–260

    CAS  Article  Google Scholar 

  • Indrani D, Soumya C, Rajiv J, Rao GV (2010) Multigrain bread—Its rheology, microstructure, quality and nutritional characteristics. J of Tex Stud 41:302–319

    Article  Google Scholar 

  • Kamaljit K, Amarjeet K, Pal TS (2011) Analysis of ingredients, functionality, formulation optimization and shelf life evaluation of high fiber bread. Am J of Food Technol 6(4):306–316

    CAS  Article  Google Scholar 

  • Kumar KA, Sharma GK, Khan MA, Semwal AD (2016) A study on functional, pasting and micro-structural characteristics of multigrain mixes for biscuits. Food Measure and Charact 10:274–282

    Article  Google Scholar 

  • Kumar KA, Sharma GK (2017) Functionality of different surfactants on the rheological and micro-structural characteristics of multigrain premix incorporated wheat flour. J of Text Stud 48:57–65

    Article  Google Scholar 

  • Kumar KA, Sudha ML (2021) Effect of fat and sugar replacement on rheological, textural and nutritional characteristics of multigrain cookies. J Food Sci Technol 58:2630–2640

    Article  Google Scholar 

  • Li S, Geng F, Wang P, Lu J, Ma M (2016) Proteome analysis of the almond kernel (Prunus dulcis). J of the Sci of Food and Agri 96:3351–3357

    CAS  Article  Google Scholar 

  • Liu X, Lu K, Yu J, Copeland L, Wang S, Wang S (2019) Effect of purple yam flour substitution for wheat flour on in-vitro starch digestibility of wheat bread. Food Chem 284:118–124

    CAS  Article  Google Scholar 

  • Liu Y, Zhou W, Young D (2010) Novel food processing. Effects on rheological and functional properties. In: Ahmed J, Ramaswamy HS, Kasapis S, Boye JI (Eds). CRC Press, Boca Raton, pp. 281–300

  • Mohamed AA, Shogren RL, Sessa DJ (2006) Low carbohydrates bread : formulation, processing and sensory quality. Food Chem 99:686–692

    CAS  Article  Google Scholar 

  • Morris C, King GE, Rubenthaler GL (1997) Contribution of wheat flour fractions to peak hot paste viscosity. Cereal Chem 74:147–153

    CAS  Article  Google Scholar 

  • Namsirilert K, Songchitsomboon S, Komindr S (2015) Effect of replacing wheat flour with coconut flour to carrot cake on in-vitro starch digestion rate and sensory evaluation. Food and Applied Biosci J 3:206–215

    Google Scholar 

  • Nishinari K, Fang Y, Guo S, Phillips GO (2014) Soy proteins : a review on composition, aggregation and emulsification. Food Hydrocoll 39:301–318

    CAS  Article  Google Scholar 

  • Noakes TD, Windt J (2017) Evidence that supports the prescription of low-carbohydrate high-fat diets: a narrative review. British J of Sports Med 51:133–139

    Article  Google Scholar 

  • Nunes MHB, Ryan LAM, Arendt EK (2009) Effect of low lactose dairy powder addition on the properties of gluten-free batters and bread quality. Euro Food Res and Technol 229:31–41

    CAS  Article  Google Scholar 

  • Olubunmi IP, Akinyele OO, Babatunde KS, Olusola OE, Adetokunbo OA, Gloria EN (2015) Influence of coconut fibre inclusion on rheological properties of composite wheat-cassava flour dough using the mixolab. J of Food and Nutri Sci 3:229–235

    Google Scholar 

  • Orsavova J, Misurcova L, Ambrozova JV, Vicha R, Mlcek J (2015) Fatty acids composition of vegetable oils and its contribution to dietary energy intake and dependence of cardiovascular mortality on dietary intake of fatty acids. Int J of Molecular Sci 16:12871–12890

    CAS  Article  Google Scholar 

  • Pamisetty A, Kumar KA, Indrani D, Singh RP (2020) Rheological, physico-sensory and antioxidant properties of punicic acid rich wheat bread. J Food Sci Technol 57:253–262

    CAS  Article  Google Scholar 

  • Pejcz E, Agnieszka M, Zygmunt G (2015) Technological characteristics of wheat and non-cereal flour blends and their applicability in bread making. J of Food and Nutri Res 54:69–78

    CAS  Google Scholar 

  • Rahaie S, Gharibzahedi SMT, Razavi SH, Jafari SM (2014) Recent developments on new formulations based on nutrient-dense ingredients for the production of healthy-functional bread: a review. J of Food Sci and Technol 51:2896–2906

    CAS  Article  Google Scholar 

  • Ray A, Prakash PK, Jyothilakshmi A, Dasappa I (2018) Modulation of carbohydrate digestibility of north Indian parotta using protein and dietary fiber based functional ingredients. Starch/staerke 70:1–8

    Article  Google Scholar 

  • Sabanis D, Tzia C (2009) Effect of rice, corn and soy flour addition on characteristics of bread produced from different wheat cultivars. Food and Biopro Technol 2:68–79

    Article  Google Scholar 

  • Sudha ML, Vetrimani R, Leetavathi K (2007) Influence of fibre from different cereal on the rheological characteristic of wheat flour dough and on biscuit quality. Food Chem 100:1365–1370

    CAS  Article  Google Scholar 

  • Szczodrak J, Pomeranz Y (1992) Starch-lipid interactions and formation of resistant starch in high-amylose barley. Cereal Chem 69:626–632

    CAS  Google Scholar 

  • Tebben L, Li Y (2018) Effect of xanthan gum on dough properties and bread qualities made from whole wheat flour. Cereal Chem 96:263–272

    Article  Google Scholar 

  • Westman EC, Yancy WS, Mavropoulos JC, Marquart M, McDuffie JR (2008) The effect of a low-carbohydrate, ketogenic diet versus a low-glycemic index diet on glycemic control in type 2 diabetes mellitus. Nutri and Metabol 5:1–9

    Article  Google Scholar 

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Authors and Affiliations



SR: Formal Analysis, Investigation; Tabulation, Writing Original Draft; AKK: Investigation, Methodology, Data curation, Validation, Supervision, Statistical analysis; Writing: Review & editing; ID: Conceptualization, Methodology, Supervision, Writing: Review & editing.

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Correspondence to K. Ashwath Kumar.

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D. Indrani: Deceased

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Rao, S., Ashwath Kumar, K. & Indrani, D. Low carbohydrate high fat flour: its rheology, bread making, physico-sensory and staling characteristics. J Food Sci Technol 59, 2220–2230 (2022).

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  • Low carbohydrate high fat (LCHF) flour
  • Coconut flour
  • Rheology
  • Bread
  • Storage study
  • Differential scanning calorimetry (DSC)