Abstract
Tempeh is a traditional Indonesian fermented food widely consumed and became staple food in some Indonesian diet. Commercially, tempeh is available in banana leaf or plastic wraps. The wraps are not only important for fermentation and giving final form of tempeh, but also potential source of food microbiome. This study aimed to investigate the effect of different packaging materials on the lactic acid bacteria (LAB) quantity and community composition of tempeh from three manufacturers with different production environments. Each tempeh wrapped with banana leaf or plastic during the fermentation process and then LAB from every tempeh sample were quantified using qPCR and plate count method. Terminal Restriction Fragment Length Polymorphism analysis was carried out to assign bacterial community composition from these samples. The LAB population quantity of fresh tempeh from three manufacturers were essentially similar around 10 log CFU/g. However, there is a difference in the proportion of cultured and uncultured bacteria. Principal Coordinate Analyses based on Bray–Curtis similarity matrices showed an apparent clustering pattern for tempeh samples according to tempeh manufacturers. Meanwhile, packaging materials did not significantly influence changes in bacterial community composition. The result of this work could be used for determination, authentication, and improvement of tempeh quality.
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References
Adegunloye DV, Agarry OO, Adebolu TT, Adetuyi FC (2006) Effect of leaf-packaging on the microbiological assessment of some food items. Afr J Biotech 5(5):445–447
Astuti M, Meliala A, Dalais FS, Wahlqvist ML (2000) Tempe, a nutritious and healthy food from Indonesia. Asia Pac J Clin Nutr 9:322–325
Babu PD, Bhakyaraj R, Vidhyalakshmi R (2009) A low cost nutritious food “tempeh”- a review. World J Dairy Food Sci 4(1):22–27
Bokulich NA, Mills DA (2012) Differentiation of mixed lactic acid bacteria communities in beverage fermentations using targeted terminal restriction fragment length polymorphism. Food Microbiol 31:126–132
Broeders S, Huber I, Grohmann L, Berben G, Taverniers I, Mazzara M, Roosens N, Morisset D (2014) Guidelines for validation of qualitative real-time PCR methods. Trends Food Sci Tech 37:115–126
Douillard FP, de Vos WM (2014) Functional genomics of lactic acid bacteria: from food to health. Microb Cell Fact 13:S8
Duval P, Cgatelard-Chauvin C, Gayard C, Rifa E, Bouchard P, Hulin S, Picque D, Montel MC (2016) Microbial dynamics in industrial blue veined cheeses in different packaging. Int Dairy J 56:198–207
Efriwati SA, Rahayu G, Nuraida L (2013) Population dynamics of yeats and lactic acid bacteria (LAB) during tempeh production. HAYATI J Biosci 20(2):57–64
Gibson GR, Saveedra JM, Mac Farlane S, Mac Farlane GT (1997) Probiotics and intestinal infections. In: Fuller R (ed) Probiotic. 2: Applications and Practical Aspects. Chapman Hall, New York, pp 10–39
Hammer Ø, Harper DA, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1):9
Haron H, Raob N (2014) Changes in micronutrient, total phenolic and anti-nutrient contents during preparation of tempeh. Nutr Food Sci. https://doi.org/10.4172/2155-9600.1000265
Hilde M, Ostile MHH, Judith NA (2003) Growth and metabolism of selected strains of probiotic bacteria in milk. Int J Food Microbiol 87(1–2):17–27
Ilha EC, Scariot MC, Treml D, Pereira TP, Sant’Anna ES, Prudencio ES, Arisi ACM (2015) Comparison of real-time PCR assay and plate count for Lactobacillus paracasei enumeration in yoghurt. Ann Microbiol 66(2):597–606
Isolauri E, Sutas Y, Kankaapaa P, Arvilommi H, Salminen S (2001) Probiotics: effects of immunity. Am J Clin Nutr 73:444–450
Jackson MS, Bird AR, Mc Orist AI (2002) Comparison of two selective media for the detection and enumeration of lactobacilli in human feces. J Microbiol Meth 51:313–321
Jeong DW, Kim HR, Jung G, Han S, Kim CT, Lee JH (2014) Bacterial community migration in the ripening of Doenjang, traditional Korean fermented soybean food. J Microbiol Biotech 24(5):648–660
Leroy F, De Vuyst L (2004) Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Tech 15(2):67–78
Levin RE (2005) The application of real-time PCR to food and agricultural systems. A rev Food Biotechnol 18:97–133
Lindow SE, Brandl MT (2003) Microbiology of the phyllosphere. Appl Environ Microb 69:1875–1883
Liu D, Tong C (2017) Bacterial community diversity of traditional fermented vegetables in China. LWT-Food Sci Technol 86:40–48
Mace S, Mamlouk K, Chipchakova S, Prevost H, Joffraud J-J, Dalgaard P, Pilet MF, Dousset X (2013) Development of a rapid real-time PCR method as a tool to quantify viable Photobacterium phosporeum bacteria in salmon (Salmo salar) steaks. Appl Environ Microb 79:2612–2619
Moreno MRF, Leisner JJ, Tee LK, Ley C, Radu S, Rusul G, Vancanneyt M, De Vuyst L (2002) Microbial analysis of Malaysian tempeh and characterization of two bacteriocins produced by isolates of Enterococcus faecium. J Appl Microbiol 92(1):789–805
Nurdini AL, Nuraida L, Suwanto A (2015) Microbial growth dynamics during tempe fermentation in two different home industries. Int Food Res J 22(4):1668
Okada N (1989) Role of microorganisms in tempe manufacture. Isolation vitamin B12 producing bacteria. JARQ 22:310–316
Pontonio E, Di Cagno R, Mahony Jennifer M, Lanera A, De Angelis M, van Sinderen D, Gobbetti M (2017) Sourdough authentication: quantitative PCR to detect the lactic acid bacterial microbiota in breads. Sci Rep-UK 7:624
Radita R, Suwanto S, Kurosawa N, Wahyudi AT, Rusmana I (2017) Metagenome analysis of tempeh production: Where did the bacterial community in tempeh come from? Malaysian J Microbiol 13(4):280–288
Santhirasegaram V, George DS, Anthony KK, Singh HKB, Saruan NM, Razali Z, Somasundram C (2016) Effects of soybean processing and packaging on the quality of commonly consumed local delicacy tempe. J Food Qual 39:675–684
Schneider S, Enkerli J, Widmer F (2009) A generally applicable assay for the quantification of inhibitory effects on PCR. J Microbiol Meth 78:351–353
Schwendimann L, Kauf P, Fieseler L, Gantenbein-Demarchi C, Schwenninger SM (2015) Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation. J Microbiol Meth 115:94–99
Smith CJ, Danilowicz BS, Clear AK, Costello FJ, Wilson B, Meijer WG (2005) T-Align, a web-based tool for comparison of multiple terminal restriction fragment length polymorphism profiles. FEMS Microbiol Ecol 54(3):375–380
Steinkraus KH, Cullen RE, Pederson CS, Nellis LF, Gavitt BK (1983) Indonesian tempeh and related fermentations. In: Steinkraus K (ed) Handbook of Indigenous Fermented Foods. Marcel Dekker, New York, pp 1–94
Van Dorst J, Bissett A, Palmer AS, Brown M, Snape I, Stark JS, Raymond B, McKinlay J, Ji M, Winsley T, Ferrari BC (2014) Community fingerprinting in a sequencing world. FEMS Microbiol Ecol 89:316–330
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This study was supported by PT Wilmar Benih Indonesia
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ME carried out the experiments and wrote the manuscript; AM supervised the work and edited the manuscript; MW helped in qPCR; AS conceived, supervised the work, and edited the manuscript.
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Erdiansyah, M., Meryandini, A., Wijaya, M. et al. Microbiological quality of tempeh with different wraps: banana leaf versus plastic. J Food Sci Technol 59, 300–307 (2022). https://doi.org/10.1007/s13197-021-05014-7
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DOI: https://doi.org/10.1007/s13197-021-05014-7