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Biodiversity of meatborne Listeria spp. in Himachal Pradesh and their interaction with indigenous probiotics

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Abstract

This study determined the anti-listerial activity of indigenous probiotics from traditional fermented foods of Western Himalaya against meat borne Listera monocytogens isolates from Himachal Pradesh. One hundred samples of meat and meat products like chicken (n = 25), chevon (goat meat, n = 20), fish (n = 20) and pork (n = 30) were collected and were analyzed for the presence of Listeria spp. by recommended culture and biochemical methods. L. monocytogens isolates were confirmed by PCR targeting the virulence gene hlyA (haemolysin A) and by16S rRNA sequencing. Anti-listerial activity of probiotic bacteria isolated from indigenous fermented foods of Himachal Pradesh was determined by well diffusion method using Lactobacillus rhamnosus GG (ATCC 53103) as the reference strain. Five percent of tested samples were found positive for L. monocytogens with incidence of 8.0% in chicken (2/25), 10.0% in fish (2/20) and 4.0% in chevon meat (1/25). None of the tested pork samples were found contaminated with L. monocytogenes. Among 11 indigenous probiotics used in this study, highest antagonistic activity was exhibited by Lactobacillus plantarum (ADF 10) and Enterococcus faecium (ADF1) which was equivalent to the reference strain.

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References

  • Allerberger F (2003) Listeria: growth, phenotypic differentiation and molecular microbiology. FEMS Immunol Med Microbiol 35:183–189

    Article  CAS  Google Scholar 

  • Anandharaj M, Sivasankari B, Rani RP (2014) Effects of probiotics, prebiotics, and synbiotics on hypercholesterolemia: a review. Chin J Biol Article ID 572754. 10.1155/2014/572754

  • Bauerfeind R, Von Graevenitz A, Kimmig P, Schiefer HG, Schwarz TF, Slenczka W, Zahner, H (2016). Listeriosis. Bacterial zoonoses. In: Zoonoses: infectious diseases transmissible between animals and humans, 4th edn, ASM Press, Washington, DC, pp 219–223

  • Becattini S, Pamer EG (2017) Multifaceted defense against Listeria monocytogenes in the gastro-intestinal lumen. Pathogens. https://doi.org/10.3390/pathogens7010001

    Article  PubMed  PubMed Central  Google Scholar 

  • Botthoulath V, Upaichit A, Thumarat U (2018) Identification and in vitro assessment of potential probiotic characteristics and antibacterial effects of Lactobacillus plantarum subsp. plantarum SKI19, a bacteriocinogenic strain isolated from Thai fermented pork sausage. J Food Sci Technol 55:2774–2785

    Article  CAS  Google Scholar 

  • Camargo AC, Todorov SD, Chihib NE, Drider D, Nero LA (2018) Lactic acid bacteria (LAB) and their bacteriocins as alternative biotechnological tools to control Listeria monocytogenes biofilms in food processing facilities. Mol Biotechnol 60:712–726

    Article  CAS  Google Scholar 

  • Castellano P, Peña N, Ibarreche MP, Carduza F, Soteras T, Vignolo G (2018) Antilisterial efficacy of Lactobacillus bacteriocins and organic acids on frankfurters. Impact on sensory characteristics. J Food Sci Technol 55:689–697

    Article  CAS  Google Scholar 

  • Cheng C, Sun J, Yu H, Ma T, Guan C, Zeng H, Zhang X, Chen Z, Song H (2020) Listeriolysin O pore-forming activity is required for ERK1/2 phosphorylation during Listeria monocytogenes infection. Front Immunol 11:1146. https://doi.org/10.3389/fimmu.2020.01146

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Clarridge JE (2004) Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 17:840–862

    Article  CAS  Google Scholar 

  • de Noordhout CM, Devleesschauwer B, Angulo FJ, Verbeke G, Haagsma J, Kirk M, Havelaar A, Speybroeck N (2014) The global burden of listeriosis: a systematic review and meta-analysis. Lancet Infect Dis 14:1073–1082

    Article  Google Scholar 

  • FAO/WHO (2002) Guidelines for the evaluation of probiotics in food. London, Ontario, Canada, April 30 and May 1. http://www.who.int/foodsafety/fs_management/en/probiotic_guidelines.pdf. Accessed 16 July 2017

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fontana C, Cocconcelli P, Vignolo G, Saavedra L (2015) Occurrence of antilisterial structural bacteriocins genes in meat borne lactic acid bacteria. Food Control 47:53–59

    Article  CAS  Google Scholar 

  • Gawade L, Barbuddhe SB, Bhosle S (2010) Isolation and confirmation of Listeria species from seafood off Goa region by polymerase chain reaction. Indian J Microbiol 50:385–389

    Article  Google Scholar 

  • Goyal C, Malik RK, Pradhan D (2018) Purification and characterization of a broad spectrum bacteriocin produced by a selected Lactococcus lactis strain 63 isolated from Indian dairy products. J Food Sci Technol 55:3683–3692

    Article  CAS  Google Scholar 

  • Gut AM, Vasiljevic T, Yeager T, Donkor ON (2018) Salmonella infection - prevention and treatment by antibiotics and probiotic yeasts: a review. Microbiology 164:1327–1344

    Article  CAS  Google Scholar 

  • Hernández-Flores KG, Vivanco-Cid H (2015) Biological effects of Listeriolysin O: implications for vaccination. Biomed Res Int 2015:360741. https://doi.org/10.1155/2015/360741

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Janda JM, Abbott SL (2007) 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J Clin Microbiol 45:2761–2764

    Article  CAS  Google Scholar 

  • Lakhanpal P, Panda AK, Thakur SD (2016) Low Prevalence of Listeria monocytogenes in ready to-eat foods of animal origin from various tourist destinations of Himachal Pradesh, India. J Commun Dis 48:29–32

    Google Scholar 

  • Lopez-Valladares G, Danielsson-Tham ML, Tham W (2018) Implicated food products for Listeriosis and changes in serovars of Listeria monocytogenes affecting humans in recent decades. Foodborne Pathog Dis 15:387–397

    Article  CAS  Google Scholar 

  • Mokta KK, Kanga AK, Kaushal RK (2010) Neonatal listeriosis: a case report from sub-Himalayas. Indian J Med Microbiol 28:385–387

    Article  CAS  Google Scholar 

  • Orsi RH, Wiedmann M (2016) Characteristics and distribution of Listeria spp., including Listeria species newly described since 2009. Appl Microbiol Biotechnol 100:5273–5287

    Article  CAS  Google Scholar 

  • Paziak-Domańska B, Bogusławska E, Wieckowska-Szakiel M, Kotłowski R, Rózalska B, Chmiela M, Kur J, Dabrowski W, Rudnicka W (1999) Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiol Lett 171:209–214

    Article  Google Scholar 

  • Radoshevich L, Cossart P (2018) Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis. Nat Rev Microbiol 16:32–46

    Article  CAS  Google Scholar 

  • Rawool DB, Malik SVS, Barbuddhe SB, Shakuntala I, Aurora R (2007) A multiplex PCR for detection of virulence associated genes in Listeria monocytogenes. Int J Food Saf 9:56–62

    Google Scholar 

  • Sanders ME, Merenstein D, Merrifield CA, Hutkins R (2018) Probiotics for human use. Nutr Bull 43:212–225

    Article  Google Scholar 

  • Saraniya A, Jeevaratnam K (2015) In vitro probiotic evaluation of phytase producing Lactobacillus species isolated from Uttapam batter and their application in soy milk fermentation. J Food Sci Technol 52:5631–5640

    Article  CAS  Google Scholar 

  • Schillinger U, Lucke FK (1989) Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol 55:1901–1906

    Article  CAS  Google Scholar 

  • Sharma S, Kanwar SS (2017) Adherence potential of indigenous lactic acid bacterial isolates obtained from fermented foods of Western Himalayas to intestinal epithelial Caco-2 and HT-29 cell lines. J Food Sci Technol 54:3504–3511

    Article  CAS  Google Scholar 

  • Silva CCG, Silva SPM, Ribeiro SC (2018) Application of bacteriocins and protective cultures in dairy food preservation. Front Microbiol 9:594. https://doi.org/10.3389/fmicb.2018.00594

    Article  PubMed  PubMed Central  Google Scholar 

  • Sourabh A, Kanwar SS, Sharma PN (2010) Diversity of bacterial probiotics in traditional fermented foods of Western Himalayas. Int J Probiotics Prebiotics 5:193–202

    Google Scholar 

  • Sourabh A, Kanwar SS, Sharma PO (2011) Antagonistic potential of indigenous bacterial probiotics of Western Himalayas against antibiotic-resistant bacterial pathogens. Curr Sci 101:1351–1356

    CAS  Google Scholar 

  • Syngai GG, Gopi R, Bharali R, Dey S, Lakshmanan GM, Ahmed G (2016) Probiotics—the versatile functional food ingredients. J Food Sci Technol 53:921–933

    Article  Google Scholar 

  • Walia S, Keshani Sood S, Kanwar SS (2014) Exhibition of DNA-bioprotective activity by microflora of traditional fermented foods of North-Western Himalayas. Food Res Int 55:176–180

    Article  CAS  Google Scholar 

  • York A (2018) Silencing Staphylococcus aureus with probiotics. Nat Rev Microbiol 16:715

    Article  CAS  Google Scholar 

  • Zhang Z, Tao X, Shah NP, Wei H (2016) Antagonistics against pathogenic Bacillus cereus in milk fermentation by Lactobacillus plantarum ZDY2013 and its anti-adhesion effect on Caco-2 cells against pathogens. J Dairy Sci 99:2666–2674

    Article  CAS  Google Scholar 

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Acknowledgements

We are thankful to CSK Himachal Pradesh Agricultural University for financial and infrastructure support for this research. Support and resources provided to Prof. S.S. Kanwar under Professor Emeritus scheme of ICAR, New Delhi are duly acknowledged.

Funding

This research work was supported by Department of Science and Technology, Govt. of India, New Delhi under its adhoc Project No. SEED/TSP/CODER/007/2012.

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Authors and Affiliations

  1. Department of Microbiology, College of Basic Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    Aakriti Sharma & S. S. Kanwar

  2. Department of Veterinary Public Health and Epidemiology, Dr. GC Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    Sidharath Dev Thakur

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  1. Aakriti Sharma
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  2. S. S. Kanwar
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Correspondence to Sidharath Dev Thakur.

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Sharma, A., Kanwar, S.S. & Thakur, S.D. Biodiversity of meatborne Listeria spp. in Himachal Pradesh and their interaction with indigenous probiotics. J Food Sci Technol 58, 1209–1215 (2021). https://doi.org/10.1007/s13197-020-04854-z

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