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Antimicrobial activity of Baccharis dracunculifolia DC and its synergistic interaction with nisin against food-related bacteria

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Abstract

The antimicrobial activities of Baccharis dracunculifolia DC essential oil (EO) and hydroalcoholic extract (HE) were evaluated. The EO showed broad antimicrobial activity and its synergistic combination with nisin was tested. Major components of EO were nerolidol, beta-pinene and D-limonene, while artepillin C, rutin and cafeic acid were major phenolics of HE. EO and HE were tested by agar diffusion assay against several strains of bacteria and yeasts, and mixed cultures of bacterial strains. The EO presented the largest spectrum of antimicrobial activity inhibiting all Gram-positive bacteria tested. Yeasts were not inhibited. The effect of EO against mixtures of sensitive and non-sensitive bacteria was tested on milk agar, being the inhibitory effect only observed on mixtures containing susceptible strains. The combination of EO and nisin at ½ MIC was evaluated on the growth curve of Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Salmonella Enteritidis during 24 h at 37 °C. The combination EO-nisin was effective and no viable counts of B. cereus, L. monocytogenes and S. Enteritidis was observed, while the individual antimicrobials caused no inhibition. The counts of S. aureus were about 4 log CFU/mL lower in comparison with EO or nisin alone. B. dracunculifolia DC may be a potential source of natural antimicrobials, and its synergistic effect with nisin would reduce the working concentration, minimizing the organoleptic effects associated with this plant antimicrobial.

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Acknowledgements

Authors thank Dr. P.M. Reque for technical support on HPLC analysis of the extracts. This work received financial support from Ministério da Ciência e Tecnologia, Ensino Superior e Técnico Profissional (MCTESTP, Mozambique) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) [Grant No 306936/2017-8].

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Authors and Affiliations

  1. Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91501-970, Brazil

    Palmira Penina Raúl Timbe, Paolo Stincone, Cristian Mauricio Barreto Pinilla & Adriano Brandelli

  2. Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, 90035-190, Brazil

    Amanda de Souza da Motta

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  1. Palmira Penina Raúl Timbe
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  2. Amanda de Souza da Motta
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  3. Paolo Stincone
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  4. Cristian Mauricio Barreto Pinilla
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  5. Adriano Brandelli
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Correspondence to Adriano Brandelli.

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Timbe, P.P.R., de Souza da Motta, A., Stincone, P. et al. Antimicrobial activity of Baccharis dracunculifolia DC and its synergistic interaction with nisin against food-related bacteria. J Food Sci Technol 58, 3010–3018 (2021). https://doi.org/10.1007/s13197-020-04804-9

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  • DOI: https://doi.org/10.1007/s13197-020-04804-9

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