Abstract
This research was carried out to evaluate the effect of viscozyme pre-treatment followed by hydrodistillation (E-HD) on extraction yield, extraction time and quality of methyl cinnamate basil (O. canum) oil. The viscozyme, as a multienzyme acting on cell wall, was used at different concentrations (0.5% and 1%, v/w) for 30, 60 and 90 min at 50 °C and pH 5 before hydrodistillation (HD). Oxygenated monoterpenes/monoterpene hydrocarbons ratio was used as a quality index for the obtained essential oil. Enzymatic pretreatment at 1% enzyme concentration for 90 min followed by HD increased yield of essential oil by 44.5% with high quality index and decreased HD time from 180 to 30 min. Thirty-nine volatile compounds, comprising > 99% of the essential oil were identified and quantified by Gas Chromatography Mass Spectrum (GC-MS). The major components of essential oil were (E)-methyl cinnamate, camphor, trans-β-caryophyllene and 1,8-cineole. Results confirm the effectiveness of the enzyme pretreatment, which enhance extraction of O. canum essential oil in a short time, with a high quality.
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References
Adam F, Vahirua-Lechat I, Deslandes E, Bessiere JM, Menut C (2009) Aromatic plants of French Polynesia. III. Constituents of the essential oil of leaves of Ocimum basilicum L. J Essent Oil Res 21:237–240. https://doi.org/10.1080/10412905.2009.9700158
Baby KC, Ranganathan TV (2016a) Effect of enzyme pre-treatment on extraction yield and quality of cardamom (Elettaria cardamomum maton.) volatile oil. Ind Crop Prod 89:200–206. https://doi.org/10.1016/j.indcrop.2016.05.017
Baby KC, Ranganathan TV (2016b) Effect of enzyme pre-treatment on extraction yield and quality of fennel (Foeniculum vulgare) volatile oil. Biocatal Agric Biotechnol 8:248–256. https://doi.org/10.1016/j.bcab.2016.10.001
Boulila A, Hassen I, Haouari L, Mejri F, Amor IB, Casabianca H, Hosni K (2015) Enzyme-assisted extraction of bioactive compounds from bay leaves (Laurus nobilis L.). Ind Crop Prod 74:485–493. https://doi.org/10.1016/j.indcrop.2015.05.050
Capurso A, Crepaldi G, Capurso C (2018) Benefits of the Mediterranean Diet in the Elderly Patient, Practical Issues in Geriatrics. Springer International Publishing AG, part of Springer Nature. https://doi.org/10.1007/978-3-319-78084-9_14
Chandran J, Padmakumari Amma KP, Menon N, Purushothaman J, Nisha P (2012) Effect of enzyme assisted extraction on quality and yield of volatile oil from black pepper and cardamom. Food Sci Biotechnol 21:1611–1617. https://doi.org/10.1007/s10068-012-0214-y
Charles DJ, Simon JE (1990) Comparison of extraction methods for the rapid determination of essential oil content and composition of basil (Ocimum spp.). J Am Soc Hortic Sci 115:458–462. https://doi.org/10.21273/jashs.115.3.458
Charoensiddhi S, Anprung P (2010) Charcaterization of bael fruit (Aegle marmelos (L.) Correa) hydrolysate as affected by enzyme treatment. J Food Biochem 34:1249–1267. https://doi.org/10.1111/j.1745-4514.2009.00333.x
Council of Europe (2005) European Pharmacopeia, 5th edn. European Council, European Directorate for the Quality of The Medicines (EDQM), Strasbourg, p 1
da Silva Moura E, D’Antonino Faroni LR, Fernandes Heleno F, Aparecida Zinato Rodrigues A, Figueiredo Prates LH, Ribeiro Lopes, de Queiroz ME (2020) Optimal extraction of Ocimum basilicum essential oil by association of ultrasound and hydrodistillation and its potential as a biopesticide against a major stored grains pest. Molecules 25:2781. https://doi.org/10.3390/molecules25122781
de Araújo Couto HGS, Blank AF, de Oliveira-e-Silva AM, de Lima Nogueira PC, de Fátima Arrigoni-Blank M, de Castro Nizio DA, Andreza de Oliveira Pinto J (2019) Essential oils of basil chemotypes: major compounds, binary mixtures, and antioxidant activity. Food Chem 293:446–454. https://doi.org/10.1016/j.foodchem.2019.04.078
Ferhat MA, Meklati BY, Smadja J, Chemat F (2006) An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. J Chromatogr A 1112:121–126. https://doi.org/10.1016/j.chroma.2005.12.030
Fun CE, Baerheim Svendsen A (1990) Composition of the essential oils of Ocimum basilicum var. canum Sims and O. gratissimum L. grown on Aruba. Flavour Fragr J 5:173–177. https://doi.org/10.1002/ffj.2730050308
Gai QY, Jiao J, Wei FY, Luo M, Wang W, Zu YG, Fu YJ (2013) Enzyme-assisted aqueous extraction of oil from Forsythia suspense seed and its physicochemical property and antioxidant activity. Ind Crop Prod 51:274–278. https://doi.org/10.1016/j.indcrop.2013.09.014
Gil-Chávez GJ, Villa JA, Ayala-Zavala JF, Heredia JB, Sepulveda D, Yahia EM, González-Aguilar GA (2013) Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview. Compr Rev Food Sci F 12:5–23. https://doi.org/10.1111/1541-4337.12005
Haider W, Sultana B, Mushtaq M, Bhatti IA (2018) Multi-response optimization of enzyme-assisted maceration to enhance the yield and antioxidant activity of Cassia fistula pods extracts. J Food Meas Charact 12:2685–2694. https://doi.org/10.1007/s11694-018-9886-1
Hosni K, Hassen I, Chaâbane H, Jemli M, Dallali S, Sebei H, Casabianca H (2013) Enzyme-assisted extraction of essential oils from thyme (Thymus capitatus L.) and rosemary (Rosmarinus officinalis L.): impact on yield chemical composition and antimicrobial activity. Ind Crop Prod 47:291–299. https://doi.org/10.1016/j.indcrop.2013.03.023
Jiao J, Fu YJ, Zu YG, Luo M, Wang W, Zhang L, Li J (2012) Enzyme-assisted microwave hydro-distillation essential oil from Fructus forsythia, chemical constituents, and its antimicrobial and antioxidant activities. Food Chem 134:235–243. https://doi.org/10.1016/j.foodchem.2012.02.114
Koroch AR, Simon JM, Juliani HR (2017) Essential oil composition of purple basils, their reverted green varieties (Ocimum basilicum) and their associated biological activity. Ind Crop Prod 107:526–530. https://doi.org/10.1016/j.indcrop.2017.04.066
Malterud KE, Farbrot TL, Huse AE, Sund RB (1993) Antioxidant and radical scavenging effects of anthraquinones and anthrones. Pharmacology 47:77–85. https://doi.org/10.1159/000139846
Mohamad N, Ramli N, Abd-Aziz S, Ibrahim MF (2019) Comparison of hydro-distillation, hydro-distillation with enzyme-assisted and supercritical fluid for the extraction of essential oil from pineapple peels. 3 Biotech 9:234. https://doi.org/10.1007/s13205-019-1767-8
Nadar SS, Rao P, Rathod VK (2018) Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review. Food Res Int 108:309–330. https://doi.org/10.1016/j.foodres.2018.03.006
Parthasarathy VA, Prasath D (2012) Cardamom. In: Peter KV (ed) Handbook of Herbs and Spices, vol 1. Second edition, Woodhead Publishing Limited, UK
Polmann G, Badia V, Frena M, Teixeira GL, Rigo E, Block JM, Feltes MMC (2019) Enzyme-assisted aqueous extraction combined with experimental designs allow the obtaining of a high-quality and yield pecan nut oil. LWT 113:108283. https://doi.org/10.1016/j.lwt.2019.108283
Rashmi Bhardwaj S, Gupta PK (2017) A comparative study of Cymbopogon citratus volatile oil by conventional method versus enzyme pre-treatment. J Essent Oil Bear Pl 20:744–751. https://doi.org/10.1080/0972060X.2017.1328289
Reis NS, Brito AR, Pacheco CSV, Costa LCB, Gross E, Santos TP, Costa AR, Silva EGP, Oliveira RA, Aguiar-Oliveira E, Oliveira JR, Franco M (2019) Improvement in menthol extraction of fresh leaves of Mentha arvensis by the application of multi-enzymatic extract of Aspergillus niger. Chem Eng Comm 206:387–397. https://doi.org/10.1080/00986445.2018.1494580
Reis NS, Santana NB, Tavares IMC, Lessa OA, Santos LR, Pereira NE, Soares GA, Oliveira RA, Oliveira JR, Franco M (2020) Enzyme extraction by lab-scale hydrodistillation of ginger essential oil (Zingiber officinale Roscoe): chromatographic and micromorphological analyses. Ind Crop Prod 146:112210. https://doi.org/10.1016/j.indcrop.2020.112210
Sakkas H, Papadopoulou C (2017) Antimicrobial activity of basil, oregano, and thyme essential oils. J Microbiol Biotechnol 27:429–438. https://doi.org/10.4014/jmb.1608.08024
Selvi MT, Thirugnanasampandan R, Sundarammal S (2015) Antioxidant and cytotoxic activities of essential oil of Ocimum canum Sims. from India. J Saudi Chem Soc 19:97–100. https://doi.org/10.1016/j.jscs.2011.12.026
Shah S, Rastogi S, Shasany AK (2018) Genomic resources of ocimum. In: Shasany AK, Kole C (Eds) The ocimum genome, Compendium of plant genomes, 1st edn. Springer Nature Switzerland AG, pp. 99–110. https://doi.org/10.1007/978-3-319-97430-9_8
Sowbhagya HB, Srinivas P, Krishnamurthy N (2010) Effect of enzymes on extraction of volatiles from celery seeds. Food Chem 120:230–234. https://doi.org/10.1016/j.foodchem.2009.10.013
Varga F, Carović-Stanko K, Ristić M, Grdiša M, Liber Z, Šatović Z (2017) Morphological and biochemical intraspecific characterization of Ocimum basilicum L. Ind Crops Prod 109:611–618. https://doi.org/10.1016/j.indcrop.2017.09.018
Vieira RF, Simon JE (2000) Chemical Characterization of basil (Ocimum Spp.) found in the markets and used in traditional medicine in Brazil. Econ Bot 54:207–216. https://doi.org/10.1007/BF02907824
Wesolowska A, Jadczak D (2016) Composition of the essential oils from inflorescences, leaves and stems of Ocimum basilicum ‘Cinnamon’ cultivated in North-western Poland. J Essent Oil Bear Pl 19:1037–1042. https://doi.org/10.1080/0972060X.2016.1197801
Wogiatzi E, Papachatzis A, Kalorizou H, Chouliara A, Chouliaras N (2011) Evaluation of essential oil yield and chemical components of selected basil cultivars. Biotechnol Biotec Eq 25:2525–2527. https://doi.org/10.5504/BBEQ.2011.0067
Zhang W, Leong SM, Zhao F, Zhao F, Yang T, Liu S (2018) Viscozyme L pretreatment on palm kernels improved the aroma of palm kernel oil after kernel roasting. Food Res Int 107:172–181. https://doi.org/10.1016/j.foodres.2018.02.023
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Morsy, N.F.S., Hammad, K.S.M. Extraction of essential oil from methyl cinnamate basil (Ocimum canum Sims) with high yield in a short time using enzyme pretreatment. J Food Sci Technol 58, 2599–2605 (2021). https://doi.org/10.1007/s13197-020-04766-y
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DOI: https://doi.org/10.1007/s13197-020-04766-y