Abstract
The stability of betalains (Bet) encapsulated in cryogels made with a mixture of albumin (ALB) and albumin-pectin (ALB-PEC) as wall materials were evaluated during storage at 32% and 83% relative humidity (RH) at several different temperature conditions (4 °C, 30 °C and 40 °C). The retention of betalains (betanin + isobetanin) and phenolic compounds and the antioxidant activity were determined by high-performance liquid chromatography, the Folin–Ciocalteu method and radical ABTS*+ capture methodology. The color parameters and images of the encapsulated betalains were obtained. Cryogels prepared with ALB at 32% RH and at 4 °C provided betanin and isobetanin retention of 72% and 82%, with half-life times of 108 and 165 days, respectively. The antioxidant activity and phenolic compounds showed retention greater than 70% during storage at 32% RH at all temperatures. Cryogels prepared with ALB-PEC also conferred high retention percentages of phenolic compounds at 83% RH, but this high RH caused a significant decrease in the retention of betalains. Both ALB and ALB-PEC improved betalain stability during storage compared with the extracts without encapsulating. Therefore, cryogels could be used as protection matrices for betalains.
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Acknowledgements
The authors are grateful to the State of São Paulo Research Foundation (Proc. FAPESP 2017/02808-6) for their financial support, the Coordination for the Improvement of Higher Education Personnel (CAPES) for a scholarship, the BIOALI Research Group (Universidad de Antioquia-Colombia), Danisco Brazil for the donation of pectin, Sensient Technologies Corporation Brazil for the donation of beet powder and Labbrands Colombia for allowing the authors to use their HPLC.
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Chaux-Gutiérrez, A.M., Pérez-Monterroza, E.J., Granda-Restrepo, D.M. et al. Effect of temperature and relative humidity on the stability of betalains encapsulated in cryogels from protein and polysaccharide. J Food Sci Technol 58, 2007–2018 (2021). https://doi.org/10.1007/s13197-020-04713-x
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DOI: https://doi.org/10.1007/s13197-020-04713-x