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Anti-obesity efficacy of the selected high altitude Himalayan herbs: in vitro studies

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Abstract

Obesity is a serious health problem for all age groups and it also promotes Type 2 diabetes, cardiovascular diseases, cancer and hypertension. Herbs are used for the weight reduction from ancient times, therefore, 12 herbs viz. Betula utilis, Bunium periscum, Angelica glauce, Saussurea lappa, Podophyllum hexandrum, Bergenia ciliate, Rumex acetosa, Aconitum heterophyllum, Centella asiatica, Andrographis paniculata, Dactylorhiza hatagirea and Bauhina variegate were selected as per literature. Rumex acetosa roots showed the highest extraction rate (39.5%), while C. asiatica leaf showed lowest extraction rate (1.25%) as compared to other herbs. As well, S. lappa leaf extract (396.52 mg GAE/g), R. acetosa root extract (181.5 mg QE/g), and B. ciliate leaf extract (79.19%) showed significantly higher Total Phenolic Content, Total Flavonoid Content and antioxidant activity, respectively. Furthermore, lipase and amylase inhibition activity and IC50 value of herb extract were higher in S. lappa root extract (75.75%, 75.41% and 62.47, 61.37, respectively). Bauhina variegate leaf extract showed a significantly higher glucose level, percentage of glucose movement (1.446 mg/dL and 63.85%) and (0.938 mg/dL and 76.55%). Based upon these experiments, 5 herbs were selected and different formulations were prepared and out of these, F3 herb formulation showed higher lipase and amylase inhibition activity (81.75% and 70.66%) and therefore, F3 herbal formulation was selected for treatment of obesity.

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Abbreviations

TPC:

Total phenolic content

TFC:

Total flavonoid content

GAE:

Gallic acid equivalent

IC50 :

Inhibitory concentration

WHO:

World Health Organisation

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Acknowledgements

The support by School of Bioengineering and Food Technology, Shoolini University, Solan, Himachal Pradesh, India is gratefully acknowledged.

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Authors and Affiliations

  1. Shoolini University, Solan, 173229, HP, India

    Mukul Kumar & Azhar Khan

  2. Lovely Professional University, Paghwara, Punjab, India

    Mukul Kumar, Samriti Guleria & Prince Chawla

  3. Amity Institute of Food Technology, Amity University, Noida, UP, 201313, India

    V. K. Modi & Ravinder Kaushik

  4. Amity University, Jaipur, Rajasthan, India

    Naveen Kumar

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  1. Mukul Kumar
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  2. Samriti Guleria
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  3. Prince Chawla
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  4. Azhar Khan
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  7. Ravinder Kaushik
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Correspondence to Ravinder Kaushik.

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Kumar, M., Guleria, S., Chawla, P. et al. Anti-obesity efficacy of the selected high altitude Himalayan herbs: in vitro studies. J Food Sci Technol 57, 3081–3090 (2020). https://doi.org/10.1007/s13197-020-04341-5

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  • DOI: https://doi.org/10.1007/s13197-020-04341-5

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