Skip to main content
Log in

Rheological and sensorial evaluation of yogurt incorporated with red propolis

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

The aim of the present study was to evaluate the use of red propolis extract, as a natural additive, in yogurt. For this, yogurt was produced with red propolis extract (YRP), to replace the additive chemical potassium sorbate, used in the commercial yogurt (CY). Analysis for apparent viscosity, texture and sensorial acceptance were performed. Apparent viscosity and texture measurements of the samples were similar to the control. Sensory evaluation showed that the samples of YRP reached a mean score of 9 on the hedonic scale, the same score found for CY. Regarding the purchase intention, the samples of YRP showed a positive intention by 64.45% of the consumers, and for CY, 68.89%. For the taste, texture, aroma and consistency, the scores were in the range from 8 to 10, for both samples. It can be concluded that the yogurt incorporated with red propolis presents potential for its commercialization in the Brazilian market.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Alencar SM, Oldoni TL, Castro ML, Cabral IS, Costa-Neto CM, Cury JA, Rosalen PL, Ikegaki M (2007) Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. J Ethnopharmacol 113:278–283. https://doi.org/10.1016/j.jep.2007.06.005

    Article  CAS  PubMed  Google Scholar 

  • Alimoradi AH, Yasini SAA, Mozaffari-Khosravi H, Daneshi M, Shirzadi A (2013) Using sesame oil as fat substitute in yogurt. World Sci J 4:208–216

    Google Scholar 

  • Anzaldúa-Morales A (1994) La evaluación sensorial de los alimentos en la teoría y la prática. Acribia SA, Zaragoza

    Google Scholar 

  • Assis DS, Brandão LV, de Sousa Costa LA, Figueiredo TV, Sousa LS, Padilha FF, Druzian JIA (2014) Study of the effects of aeration and agitation on the properties and production of xanthan gum from crude glycerin derived from biodiesel using the response surface methodology. Appl Biochem Biotechnol 172:2769–2785. https://doi.org/10.1007/s12010-014-0723-7

    Article  CAS  Google Scholar 

  • Association of Official Analytical Chemistry (AOAC) (2003) Rapid tests kits. http://www.aoac.org/tests/kits/microbiologykits.htm#salmonella. Accessed 13 April 2018

  • Association of Official Analytical Chemistry (AOAC) (2012) Official methods of analysis, 19th edn. Maryland AOAC, Gaithersburg, pp 1–3000

    Google Scholar 

  • Bernardi S, Josefina CC, Trindade MA, Akemi AC (2013) Italian-type salami with propolis as antioxidant. Ital J Food Sci 25:1–8

    Google Scholar 

  • Brasil (2001) Agência Nacional de Vigilância Sanitária (ANVISA). Resolução de Diretoria Colegiada (RDC) nº 12, 02 de janeiro de 2001. Regulamento Técnico para Padrões microbiológicos para alimentos, Brasília

  • Burdock GA (1998) Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 36:347–363

    Article  CAS  Google Scholar 

  • Cabral ISR, Oldoni TCL, Prado A, Bezerra RMN, Alencar SA, Ikegaki M, Rosalen PLC (2009) Composição fenólica, atividade antibacteriana e antioxidante da própolis vermelha brasileira. Quim Nova 32:1523–1527. https://doi.org/10.1590/S0100-40422009000600031

    Article  CAS  Google Scholar 

  • Castro IA, Vila-Real CM, Pires CIS, Pires VC, Pinto NAD, Miranda SL, Rosa CB, Dias AP (2007) Quinoa (Chenopodium quinoa Willd): digestibilidade in vitro, desenvolvimento e análise sensorial de preparações destinadas a pacientes celíacos. Alim Nutr 8:413–419

    Google Scholar 

  • Chaillou LL, Nazareno MA (2009) Bioactivity of propolis from Santiago del Estero, Argentina, related to their chemical composition. LWT-Food Sci Technol 42:1422–1427. https://doi.org/10.1016/j.lwt.2009.03.002

    Article  CAS  Google Scholar 

  • Chen J (2014) Food oral processing: some important underpinning principles of eating and sensory perception. Food Struct 9:91–105. https://doi.org/10.1016/j.foostr.2014.03.001

    Article  Google Scholar 

  • Cousin MA, Jay JM, Vasavada PC (2001) Psychrotrophic microorganisms. In: Downes FP, Ito K (eds) Compendium of methods for the microbiological examination of foods, 4th edn. American Public Health Association, Washington, DC, pp 159–166

    Google Scholar 

  • Cruz AG, Cavalcanti MR, Guerreiro LMR, Sant’Ana AS, Nogueira LC, Oliveira CAF, Deliza R, Cunha RL, Faria JAF, Bolini HAM (2013) Developing a prebiotic yogurt: rheological, physic-chemical and microbiological aspects and adequacy of survival analysis methodology. J Food Eng 114:323–330. https://doi.org/10.1016/jfoodeng.2012.08.018

    Article  CAS  Google Scholar 

  • Daugsch A, Moraes CS, Fort P, Park KY (2008) Brazilian red propolis-chemical composition and botanical origin. Evid Based Complement Alternat Med 5:347–363. https://doi.org/10.1093/ecam/nem057

    Article  Google Scholar 

  • Fernandes SS, Coelho RS, Franco RM, Barbosa CG, Luchese RH (2011) Monitoramento da microbiota de iogurtes comerciais. Rev Inst Latic Cândido Tostes 66(382):5–11

    Google Scholar 

  • Ferreira FCP, Paiva YF, Almeida RBCB, Silva ARM, Araújo AS (2015) Elaboração e avaliação sensorial de iogurte natural com polpa de abacaxi base mel. Caderno Verde de Agroecologia e Desenvolvimento Sustentável 4:99–112

    Google Scholar 

  • Freires IA, Alencar SM, Rosalen PL (2016) A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases. Eur J Med Chem 110:267–279. https://doi.org/10.1016/j.ejmech.2016.01.033

    Article  CAS  PubMed  Google Scholar 

  • Galdino PO, Galdino PO, Fernandes TKS, Oliveira MRT, Rocha APP (2010) Caracterização sensorial de iogurte enriquecido com polpa da palma forrageira (Napoleaco chenillifera). Rev Verde 5:40–53

    Google Scholar 

  • Gomes RG, Penna ALB (2009) Características reológicas e sensoriais de bebidas lácteas funcionais. Semina 30:629–646

    Google Scholar 

  • Gonsales GZ, Orsi RO, Fernandes Júnior A, Rodrigues P, Funari SRC (2006) Antibacterial activity of propolis collected in different regions of Brazil. J Venom Anim Toxins Incl Trop Dis 12:276–284

    Article  Google Scholar 

  • Gutiérrez-Cortés C, Mahecha HS (2014) Antimicrobial activity of propolis and its effect on the physicochemical and sensorial characteristics in sausages. Revista Vitae de la Facultad de Química Farmacéutica 21:90–96

    Google Scholar 

  • International Standards Organization (ISO) (2008) ISO 21527-2. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of yeasts and moulds-Part 2: colony count technique in products with water activity less than or equal to 0.95, Winterthur

  • Jansen-Alves C, Krumreich FD, Zandoná GP, Gularte MA, Borges CD, Zambiazi RC (2019) Production of propolis extract microparticles with concentrated pea protein for application in food. Food Bioprocess Technol. https://doi.org/10.1007/s11947-019-2246-2

    Article  Google Scholar 

  • Koc AN, Silici S, Mutlu-Sariguzel F, Sagdic O (2007) Antifungal activity of propolis in four different fruit juices. Food Technol Biotechnol 45(1):57–61

    CAS  Google Scholar 

  • Kročko M, Bobko M, Buchko O, Canigová M, Duchová V (2014) Sensory quality, colour and oxidative stability of cured cooked ham with propolis extract. Potravinarstvo Slovak J Food Sci 8(1):102–106. https://doi.org/10.5219/365

    Article  Google Scholar 

  • Lacerda RCS, Tiveron AP, Alencar SM (2011) Própolis e Segurança Alimentar. Segurança Alimentar e Nutricional, Campinas 18(2):99–106

    Article  Google Scholar 

  • Lancette GA, Tanini SR (2001) Staphylococcus aureus and staphylococcal enterotoxins. In: Downes FP, Ito K (eds) Compendium of methods for the microbiological examination of foods, 4th edn. American Public Health Association, Washington, DC, pp 387–403

    Google Scholar 

  • López BG, Schmidt EM, Eberlin MN, Sawaya AC (2014) Phytochemical markers of different types of red propolis. Food Chem 14:174–180. https://doi.org/10.1016/.foodchem.2013.09.063

    Article  Google Scholar 

  • López BGC, Lourenço CC, Alves DA, Machado D, Lancellotti M, Sawaya ACHF (2015) Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use. J Appl Microbiol 119(3):677–687

    Article  Google Scholar 

  • Lotti C, Campo FM, Piccinelli AL, Cuesta-Rubio O, Márquez-Hernández I, Rastrelli L (2010) Chemical constituents of red Mexican propolis. J Agric Food Chem 58:2209–2213. https://doi.org/10.1021/jf100070w

    Article  CAS  PubMed  Google Scholar 

  • Lucey JA (2002) Formation and physical properties of milk protein gels. J Dairy Sci 85:281–294. https://doi.org/10.3168/jds.S0022-0302(02)74078-2

    Article  CAS  Google Scholar 

  • Luis-Villaroya A, Espina L, García-Gonzalo D, Bayarri S, Pérez C, Pagán R (2015) Bioactive properties of a propolis-based dietary supplement and its use in combination with mild heat for apple juice preservation. Int J Food Microbiol 20:90–97. https://doi.org/10.1016/j.ijfoodmicro.2015.03.020

    Article  CAS  Google Scholar 

  • Mathias TRS, Carvalho Junior IC, Carvalho CWP, Sérvulo EFC (2011) Rheological characterization of coffee-flavored yogurt with different types of thickener. Alim Nutr 22:521–529

    Google Scholar 

  • Mendonça ICG, Porto ICCM, Nascimento TG, Souza NS, Oliveira JMS, Arruda RES, Mousinho KC, Santos AF, Basílio-Júnior ID, Parolia A, Barreto FS (2015) Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells. Evid Based Complement Alternat Med 15:357

    Article  Google Scholar 

  • Morais M, Moreira L, Feas X, Estevinho LM (2011) Honeybee-collected pollen from five Portuguese natural parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chem Toxicol 49(5):1096–1101

    Article  CAS  Google Scholar 

  • Moreira L, Dias LG, Pereira JA, Estevinho LM (2008) Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem Toxicol 46:3482–3485. https://doi.org/10.1016/j.fct.2008.08.025

    Article  CAS  PubMed  Google Scholar 

  • Nassu RT, Borba H, Verruma-Bernardi MR (2010) Validação de protocolo sensorial para avaliação de carne bovina. Braz J Food Technol 6:152–160

    Google Scholar 

  • Netíková L, Bogush P, Henrberg P (2013) Czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. J Food Sci 78:421–429. https://doi.org/10.1111/1750-3841.12230

    Article  CAS  Google Scholar 

  • Nunes LC, Galindo AB, Deus ASO, Rufino DA, Randau KP, Xavier HS, Citó AMGL, Rolim Neto PJ (2009) Seasonal variability of the constituents of propolis and bioactivity in saline Artermia. Braz J Pharmacogn 19:524–529

    Article  CAS  Google Scholar 

  • Park YK, Ikegari M, Abreu JAS, Alcici NMF (1998) Estudo da preparação dos extratos de própolis e suas aplicações. Ciênc Tecnol Alim 3:313–318

    Article  Google Scholar 

  • Polônio ML, Peres F (2009) Consumo de aditivos alimentares e efeitos à saúde: desafios para a saúde pública brasileira. Cad Saúde Pública 25(8):656–1657. https://doi.org/10.1590/S0102-311X2009000800002

    Article  Google Scholar 

  • Pontes MLC, Vasconcelos IRA, Diniza MFFM, Pessôa HLF (2018) Chemical characterization and pharmacological action of Brazilian red propolis. Acta Bras 1(1):34–39. https://doi.org/10.22571/202526-433868

    Article  Google Scholar 

  • Ramos TM, Gajo AA, Pinto SM, Abreu LR, Pinheiro AC (2009) Perfil de Textura de Labneh (Iogurte grego). Rev Inst Latic Cândido Tostes 64:8–12

    Google Scholar 

  • R Core Team (2017) Language and environment for statistical computing. R Foundation for Statistical Computing, Vienna

    Google Scholar 

  • Ribeiro VMM, Minim P, Minim RLA, Arruda AC, Ceresino EB, Carneiro HCF, Cipriano PA (2010) Estudo de mercado de iogurte da cidade de Belo Horizonte/MG. Rev Ceres 57:151–156. https://doi.org/10.1590/S0034-737X2010000200003

    Article  Google Scholar 

  • Richter RL, Vedamuthu ER (2001) Milk and milk products. In: Downes FP, Ito K (eds) Compendium of methods for the microbiological examination of foods, 4th edn. American Public Health Association, Washington, DC, pp 483–493

    Google Scholar 

  • Righ AA, Alves TR, Negri G, Marques LM, Breyer H, Salatino A (2011) Brazilian red propolis: unreported substances, antioxidant and antimicrobial activities. J Sci Food Agric 91(13):2363–2370

    Article  Google Scholar 

  • Robert NF (2008) Dossiê Fabricação de iogurte. SBRT. Rede de Tecnologia do Rio de Janeiro, REDETEC. www.sbrt.ibict.br/dossie-tecnico/downloadsDT/MzIw

  • Thamnopoulos I-AI, Michailidis GF, Fletouris DJ, Badeka A, Kontominas MG, Angelidis AS (2018) Inhibitory activity of propolis against Listeria monocytogenes in milk stored under refrigeration. Food Microbiol 73:168–176. https://doi.org/10.1016/j.fm.2018.01.021

    Article  CAS  PubMed  Google Scholar 

  • Tsukatani T, Suenaga H, Higuchi T, Akao T, Ishiyama M, Ezoe K, Matsumoto K (2008) Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts. J Microbiol Methods 75:109–116

    Article  CAS  Google Scholar 

  • Vargas-Sánchez RD, Torrescano-Urrutia GR, Acedo-Félix E, Carvajal-Millán E, González-Córdova FA, Vallejo-Galland B, Torres-Llanez M (2014) Antioxidant and antimicrobial activity of commercial propolis extract in beef patties. J Food Sci 79:1499–1504. https://doi.org/10.1111/1750-3841.12533

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazilian government (Finance Code 001), and Leonardo Fonseca Maciel and Denilson de Jesus Assis of the Pharmacy Faculty of Federal University of Bahia, and the technical team of the INSECTA laboratory from Federal University of Bahia Recôncavo for their support during the research.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rogeria Comastri de Castro Almeida.

Ethics declarations

Conflict of interest

All authors declare no conflict of interest, including financial support or other relationships with other people or organizations, within 3 years from the beginning of this submission, which may inappropriately influence or have any influence on this work.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Santos, M.S., Estevinho, L.M., de Carvalho, C.A.L. et al. Rheological and sensorial evaluation of yogurt incorporated with red propolis. J Food Sci Technol 57, 1080–1089 (2020). https://doi.org/10.1007/s13197-019-04142-5

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-019-04142-5

Keywords

Navigation