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Chemical characterization, in vitro biological activity of essential oils and extracts of three Eryngium L. species and molecular docking of selected major compounds

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Abstract

Many Eryngium species have been traditionally used as ornamental, edible or medicinal plants. The gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC–MS) analyses have shown that the major compounds in the aerial parts were spathulenol (in E. campestre and E. palmatum oils) and germacrene D (in E. amethystinum oil). The main compounds in the root oil were nonanoic acid, 2,3,4-trimethylbenzaldehyde and octanoic acid for E. campestre, E. amethystinum and E. palmatum, respectively. All the oils expressed the highest potential against Gram-positive bacteria Staphylococcus aureus as well as Gram-negative Klebsiella pneumoniae and Proteus mirabilis. Molecular docking analysis was used for determining a potential antibacterial activity mechanism of compounds present in the essential oils. Molecular docking confirmed that the binding affinity of spathulenol to the active site of tyrosyl-tRNA synthetase was the highest among the tested dominant compounds. Regarding the total phenolic content (determined by the Folin–Ciocalteu assay) and flavonoid content (evaluated using aluminum nitrate nonahydrate), the highest amount was found in the ethyl acetate extract of E. palmatum. The results of DPPH and ABTS assay indicated that the highest antioxidant activity was present in the water extract of E. amethystinum. Extracts of the aerial parts presented as minimum inhibitory concentration (MIC) expressed the activity in the range 0.004–20.00 mg/mL, with the highest activity exhibited by the acetone and ethyl acetate extracts against Proteus mirabilis. The obtained results suggest that Eryngium species may be considered a beneficial native source of the compounds with antioxidant and antimicrobial properties.

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Acknowledgements

The authors are grateful to the Ministry of Education, Science and Technological Development of the Republic of Serbia for financial support (Grant No. 173029).

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Authors and Affiliations

  1. Faculty of Medicine, University of Niš, 18000, Nis, Serbia

    Jelena S. Matejić

  2. Faculty of Science and Mathematics, University of Niš, 18000, Nis, Serbia

    Zorica Z. Stojanović-Radić & Bojan K. Zlatković

  3. Institute for Medicinal Plant Research “Dr Josif Pančić”, 11000, Belgrade, Serbia

    Mihailo S. Ristić

  4. Health Institution Filly Farm, Pharmacy Filly 63, Cara Dušana 31, 18000, Nis, Serbia

    Jovana B. Veselinović

  5. Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000, Belgrade, Serbia

    Petar D. Marin & Ana M. Džamić

Authors
  1. Jelena S. Matejić
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  2. Zorica Z. Stojanović-Radić
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  3. Mihailo S. Ristić
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  4. Jovana B. Veselinović
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  5. Bojan K. Zlatković
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  6. Petar D. Marin
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  7. Ana M. Džamić
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Correspondence to Jelena S. Matejić.

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The author Mihailo S. Ristić is deceased.

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Matejić, J.S., Stojanović-Radić, Z.Z., Ristić, M.S. et al. Chemical characterization, in vitro biological activity of essential oils and extracts of three Eryngium L. species and molecular docking of selected major compounds. J Food Sci Technol 55, 2910–2925 (2018). https://doi.org/10.1007/s13197-018-3209-8

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  • DOI: https://doi.org/10.1007/s13197-018-3209-8

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