Journal of Food Science and Technology

, Volume 55, Issue 4, pp 1541–1551 | Cite as

Metabolite characterization of different palm date varieties and the correlation with their NO inhibitory activity, texture and sweetness

  • Nur Ashikin Abdul-Hamid
  • Ahmed Mediani
  • M. Maulidiani
  • Khalid Shadid
  • Intan Safinar Ismail
  • Faridah AbasEmail author
  • Nordin H. LajisEmail author
Original Article


The aim of this study was to examine the variation in metabolite constituents of five commercial varieties of date fruits; Ajwa, Safawi and Ambar which originated from Madinah, the Iranian Bam and Tunisian Deglet Noor. The differences of metabolome were investigated using proton nuclear magnetic resonance (1H NMR) spectroscopy combined with multivariate data analysis (MVDA). Principal Component Analysis (PCA) revealed clear separation between the date varieties. The Tunisian Deglet Noor demonstrated distinct cluster from the rest of the palm date samples based on the metabolite composition as shown by the pattern observed in Hierarchical Clustering Analysis (HCA) and PCA. Deglet Noor exhibited a significant higher level of sucrose (δ 5.40) and fructose (δ 4.16) in comparison with the other four varieties which can be associated with the distinctive sweet taste of this variety. Dates originated from Madinah and Tunisia exhibited a contrast manner in the amount of xylose and moisture content. These two aspects may contribute towards the soft texture of Tunisian dates. All Madinah dates were found to contain phenolic compounds which were well established as great antioxidant and anti-inflammatory agent. Ajwa dates exerted greater effect in inhibiting the generation of nitric oxide (NO) from the stimulated RAW264.7 cells at 95.37% inhibition. Succinic acid was suggested to have the most significant correlation with the trend of NO inhibitory shown by the selected date palm varieties.


Phoenix dactylifera Ajwa dates 1H-NMR spectroscopy RAW cells Nitric oxide inhibitory activity 



The authors are gratefully acknowledged support by the Scientific Chairs Unit, Taibah University. The first author also gratefully acknowledges support from Universiti Putra Malaysia under Research University Grant Scheme (RUGS) (9301700) for funding her study under Graduate Research Fellowship.

Author contributions

F.A. and N.H.L. conceived of and designed the experiments. N.A.A.H. and A.M. performed the experiments. N.A.A.H. and M.M. analyzed the data. K.S. and I.S.I. contributed with reagents/materials/analysis and tools. N.A.A.H., N.H.L. and F.A. wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13197_2018_3073_MOESM1_ESM.docx (486 kb)
Supplementary material 1 (DOCX 485 kb)


  1. Abas F, Lajis NH, Israf DA, Khozirah S, Umi Kalsom Y (2006) Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetables. Food Chem 95:566–573CrossRefGoogle Scholar
  2. Abdul-Hamid NA, Mediani A, Maulidiani M, Abas F, Ismail IS, Shaari K et al (2016) Discrimination and nitric oxide inhibitory activity correlation of Ajwa dates from different grades and origin. Molecules 21:1–14CrossRefGoogle Scholar
  3. Abul-Soad AA, Ahmed Jatoi M, Markhand GS (2013) Performance of three Saudi Arabian date palm varieties under the agro-climatic conditions of Khairpur. Pak J Agric Sci 50:571–576Google Scholar
  4. Alam TM, Neerathilingam M, Alam MK, Volk DE, Ansari GAS, Sarkar S et al (2012) 1H-NMR nuclear magnetic resonance metabolomic study of chronic organophosphate exposure in rats. Metabolites 2:479–495CrossRefGoogle Scholar
  5. Al-Farsi MA, Lee CY (2008) Nutritional and functional properties of dates: a review. Crit Rev Food Sci Nutr 48:877–887CrossRefGoogle Scholar
  6. Al-Farsi M, Alasalvar C, Morris A, Baron M, Shahidi F (2005) Compositional and sensory characteristics of three native sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. J Agric Food Chem 53:7586–7591CrossRefGoogle Scholar
  7. Association of Official Analytical Chemists (AOAC) (2005) Official method of analysis, 18th edn. Association of Officiating Analytical Chemists, WashingtonGoogle Scholar
  8. Bray GA (2007) How bad is fructose? Am J Clin Nutr 86:895–896CrossRefGoogle Scholar
  9. Busserolles J, Rock E, Gueux E, Mazur A, Grolier P, Rayssiguier Y (2002) Short term consumption of a high-sucrose diet has a pro-oxidant effect in rats. Br J Nutr 87:337–342CrossRefGoogle Scholar
  10. Chien SC, Chen ML, Kuo HT, Tsai YC, Lin BF, Kuo YT (2008) Anti-inflammatory activities of new succinic and maleic derivatives from the fruiting body of Antrodia camphorata. J Food Agric Food Chem 56:7017–7022CrossRefGoogle Scholar
  11. El Arem A, Flamini G, Saafi Emna B, Issaoui M, Zayene N, Ferchichi A, Hammami M, Helal AN, Achour L (2011) Chemical and aroma volatile compositions of date palm (Phoenix dactylifera L.) fruits at three maturation stages. Food Chem 127:1744–1754CrossRefGoogle Scholar
  12. Eriksson L, Johansson E, Kettaneh-Wold N, Trygg J, Wikstrom C, Wold S (2006) Multi and megavariate data analysis part 1: basic principles and applications. Umetrics Inc, UmeaGoogle Scholar
  13. Georgiev MI, Ali K, Alipieva K, Verpoorte R, Choi YG (2011) Metabolic differentiation and classification of Verbascum species by NMR-based metabolomics. Phytochemistry 72:2045–2051CrossRefGoogle Scholar
  14. Hsu CL, Fang SC, Yen GC (2013) Anti-inflammatory effects of phenolic compounds isolated from the flowers of Nymphaea Mexicana Zucc. Food Funct 4:1216–1222CrossRefGoogle Scholar
  15. Joo T, Sowndhararajan K, Hong S, Lee J, Park SY, Kim S et al (2014) Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L. Saudi J Biol Sci 21:427–435CrossRefGoogle Scholar
  16. Kao FJ, Chiang WD, Liu HM (2015) Inhibitory effect of daylily buds at various stages of maturity on nitric oxide production and the involved phenolic compounds. LWT Food Sci Technol 61:130–137CrossRefGoogle Scholar
  17. Katz R, Collins ND, Cardwell AB (1961) Hardness and moisture content of wheat kernels. Cereal Chem 38:364–368Google Scholar
  18. Khare CP (2007) Indian medicinal plants: an illustrated review. Springer, New YorkGoogle Scholar
  19. Kim HS, Park SJ, Hyun SH, Yang SO, Lee J, Auh JH et al (2011) Biochemical monitoring of black raspberry (Rubus coreanus Miquel) fruits according to maturation stage by 1H NMR using multiple solvent systems. Food Res Int 44:1977–1987CrossRefGoogle Scholar
  20. Kim J, Jung Y, Song B, Bong YS, Ryu DH, Lee KS et al (2013) Discrimination of cabbage (Brassica rapa spp. pekinensis) cultivars grown in different geographical areas using 1H NMR-based metabolomics. Food Chem 137:68–75CrossRefGoogle Scholar
  21. Leleka M, Zalis’ka O, Kozyr G (2016) Screening research of pharmaceutical compositions based on succinic acid, ascorbic acid and rutin. J Pharm Pharmacol 4:486–491Google Scholar
  22. Ma T, Liasen B, Hao Q, Petersen RK, Fjaere E, Ngo HT et al (2011) Sucrose counteracts the anti-inflammatory effect of fish oil in adipose tissue and increases obesity development in mice. Fish Oil Sucrose Obes 6:1–12Google Scholar
  23. Madala NE, Piater LA, Steenkamp PA, Dubery IA (2014) Multivariate statistical models of metabolomics data reveal different metabolite distribution patterns in isonitrosoacetophenone-elicited Nicotiana tabacum and Sorghum bicolour cells. Springerplus 3:1–10CrossRefGoogle Scholar
  24. Mediani A, Abas F, Khatib A, Maulidiani H, Shaari K, Choi YH et al (2012) 1H NMR based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus. Food Res Int 49:763–770CrossRefGoogle Scholar
  25. Mohamed DA, Al-Okbi Y (2004) In vivo evaluation of antioxidant and anti inflammatory activity of date fruits in adjuvant arthritis. Pol J Food Nutr Sci 13:397–402Google Scholar
  26. Nikolic MV, Mojovic I (2008) Characterization and degradation of pectin derived from Budimka apple. J Serb Chem Soc 73:157–167CrossRefGoogle Scholar
  27. Puri A, Sahai R, Singh KL, Saxena RP, Tandon JS, Saxena KC (2000) Immunostimulant activity of dry fruits and plant materials used in Indian traditional medical system for mothers after child birth and invalids. J Ethnopharmacol 71:89–92CrossRefGoogle Scholar
  28. Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B (2007) Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in South-Eastern Morocco (Errachidia province). J Ethnopharmacol 110:105–117CrossRefGoogle Scholar
  29. Taylor JLS, van Staden J (2001) The effect of age, season and growth conditions on anti-inflammatory activity in Eucomis autumnalis (Mill.) Chitt. plant extract. Plant Growth Regul 34:39–47CrossRefGoogle Scholar
  30. Xia J, Sinelnikov IV, Beomsoo H, Wishart DS (2015) Metaboanalyst 3.0: making metabolomics more meaningful. Nucleic Acids Res 37:652–660CrossRefGoogle Scholar
  31. Yang D, Xiaoxing W, Wu Y, Lu B, Yuan A, Leon C et al (2015) Urinary metabolomic profiling reveals the effect of shenfu decoction on chronic heart failure in rats. Molecules 20:11915–11929CrossRefGoogle Scholar
  32. Zhang CH, Aldosari SA, Vidyasagar PSPV, Nair KM, Nair MG (2013) Antioxidant and anti-inflammatory assays confirm bioactive compounds in Ajwa date fruit. J Agric Food Chem 61:5834–5840CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2018

Authors and Affiliations

  1. 1.Laboratory of Natural Products, Institute of BioscienceUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of Food Science, Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Chemistry, Faculty of ScienceIslamic University in MadinahAlmadinah AlmonawarahSaudi Arabia
  4. 4.Department of Chemistry, Faculty of ScienceUniversiti Putra Malaysia (UPM)SerdangMalaysia

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