Abstract
This paper reports the prediction capacity of various microbial inactivation models to prefigure the bactericidal effect using pulsed electric field (PEF) on liquid food. The aim of study was to compare the various inactivation models based on accuracy and bias factor to find out the most accurate inactivation model for Escherichia coli present in carrot juice treated with PEF. In this study, E. coli suspended in carrot juice was treated with varying pulsed electric field strength for different intervals. The obtained data were utilized for the evaluation of parameters of Bigelow, Peleg, Hülsheger and Weibull inactivation models. Furthermore, secondary models were developed for Hülsheger and Weibull to predict the microbial inactivation at any level of field strength and treatment time. The secondary model for Hülsheger exhibits 5.8% error as compared to the Weibull model having 8.5% error in prediction of death kinetics of E. coli suspended in carrot juice by means of PEF. The comparative analysis of secondary models to forecast the unknown data set unveiled the superior functioning of Hülsheger model.
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Notes
With ingredients per litter: Peptic digest of animal tissue 5 gm, Sodium chloride 5 gm, Beef extract 1.5 gm, Yeast extract 1.5 gm, and Agar 15 gm with final pH (at 25 °C) 7.4 ± 0.2.
With ingredients per litter: Pancreatic Digest of Gelatin 10 gm, Lactose 10 gm, Dipotassium Phosphate 2 gm, Eosin Y 0.4 gm, Methylene Blue 65 mg and Agar 15 gm.
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Acknowledgements
This study was carried out at CSIR-CSIO Chandigarh, India. We are very thankful to Director CSIR-CSIO for providing finical support for this research work. We thank Dr. H. K Sardana (Chief Scientist, CSIR-CSIO) and Dr. G. S Prasad (Chief Scientist, CSIR-IMTECH) for valuable discussions.
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Singh, J., Singh, M., Singh, B. et al. Comparative analyses of prediction models for inactivation of Escherichia coli in carrot juice by means of pulsed electric fields. J Food Sci Technol 54, 1538–1544 (2017). https://doi.org/10.1007/s13197-017-2585-9
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DOI: https://doi.org/10.1007/s13197-017-2585-9