Skip to main content
Log in

Development of protein fortified mango based ready-to-serve beverage

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Fruit drinks contain negligible amount of protein as nutritional component. Fortification of fruit drinks with protein is a challenge due to protein stability in acidic and ionic environment. Mango ready-to-serve (RTS) beverage was fortified with modified whey protein and its rheological properties were studied. Whey protein was hydrolysed with papain to improve its stability in acidic medium. The water holding capacity of whey protein increased about two times after hydrolysis. Hydrolysed and native whey protein was used at 2, 3 and 4% levels for fortification of mango based RTS beverage. Addition of hydrolysed whey protein at all the three levels did not significantly change the flow behaviour of the beverage. Native whey protein fortification resulted in precipitation; however, addition of hydrolysed whey protein led to stable beverage formulation at all the three levels. Hydrolysed whey protein imparted slight bitter taste to the RTS beverage, which was masked by β-cyclodextrin @ 0.15% of total protein. The mango RTS beverage with 3.0% hydrolysed whey protein was found acceptable with good sensory appeal and stability during thermal processing as well storage in glass bottles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • AOAC (1990) Official methods of analysis, 15th edn. Association of official analytical chemists, Washington

    Google Scholar 

  • Beuchat LR (1977) Functional and electrophoretic characteristics of succinylated peanut flour protein. J Agric Food Chem 25:258–261

    Article  CAS  Google Scholar 

  • Chobert JM, Bertrand HC, Nicolas MG (1988) Solubility and emulsifying properties of caseins and whey proteins modified enzymatically by trypsin. J Agric Food Chem 36:883–892

    Article  CAS  Google Scholar 

  • DoAC (2015) Agricultural statistics at a glance. Directorate of Economics and Statistics, Department of Agriculture and Cooperation, Ministry of Agriculture, Government of India, New Delhi

  • Flanagan J, FitzGerald RJ (2002) Physicochemical and nitrogen solubility properties of Bacillus proteinase hydrolysates of sodium caseinate incubated with transglutaminase pre-and post-hydrolysis. J Agric Food Chem 50:5429–5436

    Article  CAS  Google Scholar 

  • Foegeding EA, Davis JP, Doucet D, McGuffey MK (2002) Advances in modifying and understanding whey protein functionality. Trends Food Sci Technol 13(5):151–159

    Article  CAS  Google Scholar 

  • Goudarzi M, Madadlou A, Mousavi ME, Emam-Djomeh Z (2015) Formulation of apple juice beverages containing whey protein isolate or whey protein hydrolysate based on sensory and physicochemical analysis. Int J Dairy Technol 68(1):70–78

    Article  CAS  Google Scholar 

  • Graessley WW (1974) Advances in polymer science. Springer-Verlag, New York

    Google Scholar 

  • Harringan WF, McCance ME (1976) Laboratory methods in food microbiology. Academic Press, London

    Google Scholar 

  • Hosseini-Parvar HS, Matia-Merino L, Goh KKT, Razavi SMA, Mortazavi SA (2010) Steady shear flow behaviour of gum extracted from Ocimum basilicum L. Seed: effect of concentration and temperature. J Food Eng 101:236–243

    Article  Google Scholar 

  • Jeewanthi CR, Paik HD, Kim MH, Lee NK, Kim SY, Yoon CY (2014) Characteristics of whey protein hydrolysates from cheese whey, favours on various food applications. Chem Ind Chem Eng 20:503–509

    Article  Google Scholar 

  • Jeewanthi RKC, Lee NK, Paik HD (2015) Improved functional characteristics of whey protein hydrolysates in food industry. Korean J Food Sci Anim Resour 35(3):350–359

    Article  Google Scholar 

  • Kneifel W, Seiler A (1993) Water-holding properties of milk protein products-A review. Food Struct 12(3):297–308

    CAS  Google Scholar 

  • Larmond E (1977) Laboratory methods for sensory evaluation of foods. Canada Dept of Agric Publication, Ottawa, pp 1637–1662

    Google Scholar 

  • Lee CA, Vickers ZM (2008) The astringency of whey protein beverages is caused by their acidity. Int Dairy J 18:1153–1156

    Article  CAS  Google Scholar 

  • Marcotte M, Taherian AR, Ramaswamy HS (2001) Rheological properties of selected hydrocolloids as a function of concentration and temperature. Food Res Int 34:695–704

    Article  CAS  Google Scholar 

  • Rittmanic S (2006) US whey proteins in ready-to-drink beverages. US Dairy Export Council, Arlington

    Google Scholar 

  • Ryan KN, Vardhanabhuti B, Jaramillo DP, van Zanten JH, Coupland JN, Foegeding EA (2012) Stability and mechanism of whey protein soluble aggregates thermally treated with salts. Food Hydrocoll 27:411–420

    Article  CAS  Google Scholar 

  • Segall K (2009) Protein fortification of acidic beverages: a clear opportunity. Food and Beverage Asia August/September, pp 31–33

  • Sharma SK, Zhang QH, Chism GW (1998) Development of a protein fortified fruit beverage and its quality when processed with pulsed electric field treatment. J Food Quality 21:459–473

    Article  Google Scholar 

  • Silvestre MP, Silva MR, Silva VD, Souza MW, Junior L, de Oliveira C, Afonso WD (2012) Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity. Braz J Pharma Sci 48:747–757

    Article  CAS  Google Scholar 

  • Sizer FS, Whitney EN (1994) The proteins and amino acids: nutrition concepts and controversies. West Publishing Co, New York, pp 180–212

    Google Scholar 

  • Sopade PA, Halley PA, Cichero JAY, Ward LC (2007) Rheological characterisation of food thickeners marketed in Australia in various media for the management of dysphagia. I: water and cordial. J Food Eng 79:69–82

    Article  Google Scholar 

  • Stoliar M (2009) Whey products in baked goods applications monograph—Bakery US Dairy Export Council. Arlington, VA, pp 1–8

    Google Scholar 

  • USP (1989) Inc: The United States Pharmacopeia, 22nd Revision. Mack Publishing Company, Easton, Pennsylvania

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Deep N. Yadav.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yadav, D.N., Vishwakarma, R.K., Borad, S. et al. Development of protein fortified mango based ready-to-serve beverage. J Food Sci Technol 53, 3844–3852 (2016). https://doi.org/10.1007/s13197-016-2395-5

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-016-2395-5

Keywords

Navigation