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Functional characterization and microencapsulation of probiotic bacteria from koozh

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Abstract

Koozh is a traditional fermented millet beverage unique to south India. Analysis of six market samples of koozh for their microbial profile resulted in 69 isolates of presumptive lactic acid bacteria (LAB). They were grouped as Leuconostoc sp., Enterococcus sp., Streptococcus sp. and Lactobacillus sp. based on morphological characteristics and biochemical tests. Eight among them showed probiotic features: resistance to acid (2.5 pH for 6 h), resistance to 0.3 % ox bile, moderate hydrophobicity (40 %), antibacterial activity against 10 pathogens, susceptibility to 50 % of antibiotics tested. Sequencing of 16srDNA showed them to be five strains of Enterococcus hirae and one each of Enterococcus facecalis, Bacillus amyloliquefaciens and Lactobacillus plantarum. The probiotic isolates were encapsulated in skim milk powder by two different drying techniques: freeze drying and spray drying. The encapsulated probiotic isolates survived both in simulated gastric fluid and simulated intestinal fluid with high cell viability (98–99 %). Storage for 16 weeks at room temperature (27 °C), resulted in 2 log reduction, but better survival with only 1 log reduction was observed at 4 °C and was best at −20 °C. Survival of isolates was similar in both spray and freeze dried products.

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Acknowledgments

This work was supported by the University Grants Commission-UGC, Rajiv Gandhi National Fellowship No.F.14-2 (SC) /2009 (SA-III) (www.ugc.ac.in/rgnf/), 2009 a doctoral studies grant, provided by the Government of India, Ministry of Social Justice and Empowerment, and Ministry of Tribal Affairs.

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Authors and Affiliations

  1. Centre for Food Technology, Anna University, Sardar Patel Road, Chennai, Tamil Nadu, 600025, India

    Shankar Ilango & Usha Antony

  2. Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, 723102, India

    Ruby Pandey

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  1. Shankar Ilango
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  2. Ruby Pandey
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  3. Usha Antony
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Correspondence to Usha Antony.

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Ilango, S., Pandey, R. & Antony, U. Functional characterization and microencapsulation of probiotic bacteria from koozh . J Food Sci Technol 53, 977–989 (2016). https://doi.org/10.1007/s13197-015-2169-5

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