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Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities

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Abstract

Artemisia selengensis Turcz (AST) is a perennial herb with therapeutic and economic applications in China. The effects of ultrasound-assisted extraction (UAE) parameters upon extraction yield (EY%), antioxidant and antitumor activities of the polysaccharides extracts were studied by using a factorial design and response surface methodology. The optimal conditions determined were as: ultrasonic power 146 W, extraction time 14.5 min. and extraction temperature 60 °C. The average molecular weights of two homogeneous polysaccharides (APS1 and APS2) purified by DEAE cellulose-52 and Sephadex G-100 column chromatography were 125.4 and 184.1 kDa, respectively. Monosaccharide analysis showed that APS1 and APS2 were composed of five common monomers i.e., galactose, mannose, arabinose, xylose and rhamnose and one different monomer glucose and galacturonic acid respectively, with a most abundant part in molar % of APS1 and APS2 were glucose (83.01 %) and galacturonic acid (48.87 %) while least were xylose (0.80 %) and mannose (1.73 %) respectively. The antioxidant properties were determined by evaluating DPPH, hydroxyl radical scavenging activity and reducing power which indicated both APS1 and APS2 showed strong scavenging activities and anticancer activities on HT-29, BGC823 and antitumor activity on HepG-2. As UAE improved the polysaccharides yield than CSE, meanwhile, no significant difference of polysaccharides chemical compositions. Therefore, the present study suggests that the consumption of AST leaves may beneficial for the treatment of many diseases.

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Acknowledgments

All authors are grateful to Jinhu Qinrong Tea factory, for providing fresh experimental materials. We are also thankful to Instrumental Analysis of CPU Center of China Pharmaceutical University for technical assistance.

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    Authors and Affiliations

    1. College of Food Science and Technology, Nanjing Agricultural University, Weigang, Nanjing, 210095, People’s Republic of China

      Juan Wang, He Dong Lu, Umair Muḥammad, Jin Zhi Han, Zhao Hui Wei, Zhao Xin Lu, Xiao Mei Bie & Feng Xia Lu

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    1. Juan Wang
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    2. He Dong Lu
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    3. Umair Muḥammad
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    Correspondence to Feng Xia Lu.

    Additional information

    Juan Wang and He Dong Lu contributed equally to this work.

    Research highlights

    1. UAE could efficiently improved the polysaccharides yield from AST

    2. Two polysaccharides (APS1 and APS2) were separated from AST

    3. APS1 and APS2 both showed strong antioxidant properties

    4. Both APS1 and APS2 showed strong anticancer activities

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    Cite this article

    Wang, J., Lu, H.D., Muḥammad, U. et al. Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities. J Food Sci Technol 53, 1025–1034 (2016). https://doi.org/10.1007/s13197-015-2156-x

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    • DOI: https://doi.org/10.1007/s13197-015-2156-x

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