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Optimization of compositional and structural properties in probiotic sausage production

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Abstract

In this study, the effects of different fat levels and different particle sizes on compositional and structural characteristics of probiotic fermented sausage were investigated. In order to obtain probiotic character, Lactobacillus casei CRL431 was added. The physicochemical, microbiological and sensory analysis were done. The effect of fat level x mincer hole diameter interaction on hardness values were statistically significant (p < 0.005). At the end of the fermentation-ripening period, L.casei CRL-431 count has reached to sufficient microbial count (106 cfu/g of probiotic bacteria) to demonstrate the character of probiotic food. A significant positive correlation was found between L.casei CRL431 count and surface appearance, texture and overall acceptability scores (r = 0.60, 0.52, 0.53). The values of TBARS number of probiotic sucuk samples increased during fermentation-ripening. A significant correlation between taste-aroma scores and fat level was detected (r = −0.61,p = 0.0008). Consiquently, the best sensorial quality was determined in L3 samples and the worst sensorial quality was determined in H8 samples.

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References

  • Ahmad S, Sristava PK (2007) Quality and shelf life evaluation of fermented sausages of buffalo meat with different levels of heart and fat. Meat Sci 75:603–609

    Article  CAS  PubMed  Google Scholar 

  • Akkaya L, Gök V, Kara R, Yaman H (2014) Enterotoxin production by Staphylococcus aureus (a, B, C, D) during the ripening of sucuk (Turkish dry-fermented Sausage). CyTA-J Food 12(2):127–133

    Article  CAS  Google Scholar 

  • AOAC (1984) Official methods of analysis, Centennial edn. Association of Official Analytical Chemists Washington D.C, USA

    Google Scholar 

  • AOAC (2000) Official Methods of Analysis of AOAC International (17.Edition). USA

  • Bağdatli A, Kayaardi S (2015) Influence of storage period and packaging methods on quality attributes of fresh beef steaks. CyTA - Journal of Food 13(1):124–133

    Article  CAS  Google Scholar 

  • Bozkurt H, Bayram M (2006) Color and textural attributes of sucuk during ripening. Meat Sci 73:344–350

    Article  PubMed  Google Scholar 

  • Bruna JM, Fernandez M, Hierro EM, Ordonez JA, de la Hoz L (2000) Combined use of pronase and fungal extract (Penicillium aurantiogriseum) to potantiate the sensory characteristics of dry fermented sausages. Meat Sci 54:135–145

    Article  CAS  PubMed  Google Scholar 

  • Chr Hansen (2005) L.acidophilus, L.casei and Bifidobacteria in Fermented Milk Products. Guidelines method for counting probiotic bacteria. Bulletin F-6 LA LC BB March2005/3:8, Chr. Hansen, Denmark

  • Dalmış Ü (2007) Sucukta üretim ve depolama sırasında meydana gelen mikrobiyolojik ve biyokimyasal değişmeler. Doktora tezi Ankara Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı, Ankara, pp. 1–123

    Google Scholar 

  • Ercoşkun H, Özkal SG (2011) Kinetics of traditional Turkish sausage quality aspects during fermentation. Food Control 22:165–172

    Article  CAS  Google Scholar 

  • Ergönül B, Kundakçı A (2011) Microbiological attributes and biogenic amine content of probiotic Turkish fermented sausage. J Verbr Lebensm 6(1):49–56

    Article  CAS  Google Scholar 

  • Ergönül B (2009) Farklı probiyotik kültürler kullanılarak Hindi sucuğu üretimi ve kalite üzerine etkileri. Doktora tezi Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı, Manisa

    Google Scholar 

  • Gençcelep H (2006) Sucuk üretiminde değişik starter kültürler ve farklı nitrit seviyelerinin biyojen Amin oluşumu üzerine etkileri. Doktora Tezi Atatürk Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği ABD, Erzurum

    Google Scholar 

  • Gök V (2006) Antioksidan kullanımının fermente sucukların bazı kalite özellikleri üzerine etkileri. Doktora Tezi Ankara Üniversitesi Gıda Mühendisliği Anabilim Dalı, Ankara, pp. 1–110

    Google Scholar 

  • Gönülalan Z, Arslan A, Köse A (2004) Farklı starter kültür kombinasyonlarının fermente sucuklardaki etkileri. Turk J Vet Anim Sci 28:7–16

    Google Scholar 

  • Harrigan WF (1998) Laboratory methods in food microbiology. Academic Press, London, pp. 1–532

    Google Scholar 

  • Jaworska D, Neffe K, Kolozyn-Krajewska D, Dolatowski Z (2011) Survival during storage and sensory effect of potential probiotic lactic acid bacteria Lactobacillus acidophilus Bauer and Lactobacillus casei Bif3’/IV in dry fermented pork loins. Int J Food Sci Technol 1365-2621:2011.02772

    Google Scholar 

  • Kandemir Can BD (2001) Fermente türk sucuğu üretiminde probiyotik bakterilerin kullanımı. Yüksek Lisans Tezi, Ankara, pp. 1–56

    Google Scholar 

  • Kayaardı S, Gök V (2003) Effect of replacing beef fat with olive oil on quality characteristics of Turkish soudjouk (sucuk). Meat Sci 66:249–257

    Article  CAS  Google Scholar 

  • Kołożyn-Krajewska D, Dolatowski ZJ (2009) Probiotics in fermented meat products. Acta Sci Pol Technol Aliment 8(2):61–74

    Google Scholar 

  • Muguerza E, Fista G, Ansorena D, Astiasaran I, Bloukas JG (2002) Effect of fat level and partial replacement of pork backfat with olive oil on processing and quality characteristics of fermented sausages. Meat Sci 61/4:397–404

  • Olivares A, Navarro JL, Salvador A, Flores M (2010) Sensory acceptability of slow fermented sausages based on fat content and ripening time. Meat Sci 86:251–257

    Article  PubMed  Google Scholar 

  • Papadima SN, Bloukas JG (1999) Effect of fat level and storage conditions on quality characteristics of traditional Greek sausages. Meat Sci 51:103–113

    Article  CAS  PubMed  Google Scholar 

  • Pichhardt K (1993) Lebensmittel-mikrobiologie. Springer Verlag, Berlin

    Google Scholar 

  • Radulovic Z, Zivkovic D, Mirkovic N, Petrusic M, Stajic S, Perunovic M, Paunovic D (2011) Effect of probiotic bacteria on chemical composition and sensory quality of fermented sausages. Procedia Food Science 1:1516–1522

    Article  CAS  Google Scholar 

  • Rouhı M, Sohrabvandı S, Mortazavıan AM (2013) Probiotic fermented sausage: viability of probiotic microorganisms and sensory characteristics. Crit Rev Food Sci Nutr 53:331–348

    Article  CAS  PubMed  Google Scholar 

  • Sante V, Fernandez X (2000) The measurement of pH in raw and frozen Turkey pectoralis supercialis muscle. Meat Sci 55:503–506

    Article  CAS  PubMed  Google Scholar 

  • Sayas-Barbera E, Viuda-Martos M, Fernandez-Lopez F, Perez-Alvarez JA, Sendra E (2012) Combined use of a probiotic culture and citrus fiber in a traditional sausage ‘longaniza de Pascua’. Food Control 27:343–350

    Article  CAS  Google Scholar 

  • Sidira M, Karapetsas A, Galanis A, Kanellaki M, Kourkoutas Y (2014) Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics. Meat Sci 96:948–955

    Article  CAS  PubMed  Google Scholar 

  • Soyer A (2005) Effect of fat level and repining temperetaure on biochemical and sensory characteristics of naturally fermented Turkish sausages (sucuk). Eur Food Res Technol 221:412–415

    Article  CAS  Google Scholar 

  • Tarladgis BG, Watts BM, Yonathan M (1960) Distillation method for the determination of malonaldehyde in rancid foods. J Am Oil Chem Soc 37(1):44–48

    Article  CAS  Google Scholar 

  • Toldra F, Reig M (2011) Innovations for healthier processed meats. Trends Food Sci Technol 22:517–522

    Article  CAS  Google Scholar 

  • Trzasowska M, Kolozyn-Krajewska D, Wojciak KM, Dolatowski Z (2014) Microbiological quality of raw-fermented sausages with Lactobacillus casei LOCK 0900 probiotic strain. Food Control 35:184–191

    Article  Google Scholar 

  • Vural H, Öztan A (1996) Et ve Et Ürünleri Kalite Kontrol Laboratuarı Uygulama Klavuzu. Hacettepe Üniversitesi Mühendislik Fak. Yayınları Yayın No: 36, Ankara, 1–235

  • Wojciak KM, Dolatowski ZJ, Kolozyn-Krajewska D, Trzasowska M (2012) The effect of the Lactobacillus casei lock0900 probiotic strain on the quality of dry-fermented sausage during chilling storage. J Food Qual 35:353–365

    Article  CAS  Google Scholar 

  • Yalınkılıç B, Kaban G, Kaya M (2012) The effects of different levels of orange fiber and fat on microbiological, physical, chemical and sensorial properties of sucuk. Food Microbiol 29:255–259

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by “Celal Bayar University Research Project Funds,” Turkey under Grant Project number 2011-022. The authors thank PINAR Integrated Meat and Feed Industries Inc., Kemalpaşa, İzmir for the manufacture of the sucuk.

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Correspondence to Aytunga Bağdatli.

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Bağdatli, A., Kundakci, A. Optimization of compositional and structural properties in probiotic sausage production. J Food Sci Technol 53, 1679–1689 (2016). https://doi.org/10.1007/s13197-015-2098-3

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  • DOI: https://doi.org/10.1007/s13197-015-2098-3

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