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Antioxidant activities and functional properties of enzymatic protein hydrolysates from defatted Camellia oleifera seed cake

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Abstract

Seed cake protein (SCP) from Camellia oleifera was hydrolyzed by five commercial proteases (Flavorzyme, Trypsin, Neutrase, Papain, Alcalase). Amino acid composition, molecular weight distribution, antioxidant activity and functional property of the seed cake protein hydrolysates (SCPH) were investigated. Enzymatic hydrolysis improved protein solubility significantly but impaired the foaming and emulsifying property. Hydrolysate generated by alcalase had the highest hydrolysis degree (DH) and antioxidant activity, and displayed excellent protein solubility over wide range of pH, while hydrolysate prepared by flavorzyme showed better copper chelating capacity and emulsifying stability with low molecular weight distribution. Trypsin-treated SCPH showed better foaming property than original protein. The results indicated that enzyme type greatly influenced the molecular weight, functional property and antioxidant activity of SCPH. It was also found that electing appropriate protease and controlling the DH could be enhanced or reduced functional property according to actual applications.

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Acknowledgments

The authors gratefully acknowledge the financial support by Department of Science and Technology Support Project (2013NZ0047) of Sichuan Province. The authors sincerely thank for the assistance of Xuejing Jia and Haoran Cheng.

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Authors and Affiliations

  1. College of Life Science, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Xu Li, Junlin Deng, Shian Shen, Ming Yuan, Ruiwu Yang & Chunbang Ding

  2. College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    Tian Li

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  1. Xu Li
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  2. Junlin Deng
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  3. Shian Shen
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  4. Tian Li
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  5. Ming Yuan
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  6. Ruiwu Yang
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  7. Chunbang Ding
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Correspondence to Chunbang Ding.

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Li, X., Deng, J., Shen, S. et al. Antioxidant activities and functional properties of enzymatic protein hydrolysates from defatted Camellia oleifera seed cake . J Food Sci Technol 52, 5681–5690 (2015). https://doi.org/10.1007/s13197-014-1693-z

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  • DOI: https://doi.org/10.1007/s13197-014-1693-z

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