Abstract
Menaquinone 7 (MK-7) is nutritionally important metabolite found by fermentation mainly using B. subtilis species. In this study, soybean medium was modified to improve the MK-7 production using Bacillus subtilis NCIM 2708 under solid state fermentation. The objective of this study was to produce large amount of MK-7 within a short period of time. Nine nutritional components viz. glycerol, mannitol, dextrose, sucrose, yeast extract, malt extract, K2HPO4, MgSO4.7H2O and CaCl2 were investigated to obtain the maximum MK-7 concentration. The highest MK-7 concentration 39.039 μg/g was obtained after 24 h of fermentation in the following optimised medium components: soybean 20 g, glycerol 40 ml/kg, mannitol 60 g/kg, yeast extract 4 g/kg, malt extract 8 g/kg and calcium chloride 4 g/kg. The maximum production of MK-7 56.757 μg/g was predicted by point prediction tool of Design Expert 7.1 software (Statease Inc. USA). This data shows 68.78 % validity of the predicted model.
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Acknowledgments
The authors gratefully Professor S. K. Lal, IARI, PUSA, New Delhi for providing soybeans variety. All the authors acknowledge the Department of Science and Technology (DST), Govt. of India for providing fellowship for scholar under INSPIRE Programme.
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Rishipal Singh, Alka Puri and Bibhu Prasad Panda have contributed equally to this paper
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Singh, R., Puri, A. & Panda, B.P. Development of menaquinone-7 enriched nutraceutical: inside into medium engineering and process modeling. J Food Sci Technol 52, 5212–5219 (2015). https://doi.org/10.1007/s13197-014-1600-7
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DOI: https://doi.org/10.1007/s13197-014-1600-7