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Potential utilization of Citrullus lanatus var. Colocynthoides waste as a novel source of pectin

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Abstract

The Citrullus lanatus var. Colocynthoides is an ancestor type of watermelon. It was investigated as a new source of pectin. It was cultivated in Egypt for seeds only, while the remaining fruits are discarded as waste. Effect of different extraction conditions such as pH, solid: liquid ratio, temperature and extraction time on pectin yield of Citrullus lanatus var. Colocynthoides waste was investigated in the present study. The highest yield (19.75 % w/w) was achieved at pH 2, solid: liquid ratio1:15 and 85 °C, for 60 min. Methylation degree and galacturonic acid content of extracted pectin were 55.25 %, w/w and 76.84 %, w/w. The main neutral sugars were galactose followed by arabinose and rhamnose. In addition, glucose, xylose and mannose existed as constituents in the pectin hydrolysate. The results indicated that Citrullus lanatus var. Colocynthoide waste is a potential new source of pectin.

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References

  • AOAC (2000) Official methods of analysis, 17th edn. Association of official Analytical Chemist, USA

    Google Scholar 

  • Bottger I (1990) Pectin application some practical problems. In: Phillips GO, Wedlock DJ, Williams PA (eds) Gums and stabilisers for the food industry, Oxford, UK, pp 247–256

  • Brown L, Rosner B, Willett WW, Sacks FM (1999) Cholesterol lowering effects of dietary fiber: a meta-analysis. Am J Clin Nutr 69:30–42

    CAS  Google Scholar 

  • Canteri-Schemin MH, Fertonani HCR, Waszczynskyj N, Wosiacki G (2005) Extraction of pectin from apple pomace. Braz Arch Biol Technol 48(2):259–266

    Article  CAS  Google Scholar 

  • Chakraborty A, Ray S (2011) Development of a process for the extraction of pectin from citrus fruit wastes viz. lime peel, spent guava extract, apple pomace etc. Internet J Food Safety 13:391–397

    Google Scholar 

  • Chang KC, Dhurandhar N, You X, Miyamoto A (1994) Sunflower head residue pectin extraction as affected by physical conditions. J Food Sci 59(6):1207–1210

    Article  CAS  Google Scholar 

  • Cho SW, Lee S, Shin W (2001) The X-ray structure of Aspergillus aculeatus polygalacturonases and a modeled structure of the polygalacturonase- octagalacturonate complex. J Mol Biol 314:863–878

    Article  Google Scholar 

  • Endress HU, Christensen SH (2009) Pectins. In: Phillips GO, Williams PA (eds) Handbook of hydrocolloids, 2nd edn. Woodhead, Cambridge, UK, pp 274–279

  • Faravash RS, Ashtiani FZ (2008) The influence of acid volume, ethanol-to-extract ratio and acid-washing time on the yield of pectic substances extraction from peach pomace. Food Hydrocoll 22:196–202

    Article  CAS  Google Scholar 

  • Fishman ML, Pfeffer PE, Barford RA, Doner LW (1984) Studies of pectin solution properties by high-performance size exclusion chromatography. J Agric Food Chem 32:372–378

    Article  CAS  Google Scholar 

  • Garna H, Mabon N, Robert C, Cornet C, Nott K, Legros H, Wathelet B, Paquot M (2007) Effect of extraction conditions on the yield and purity of apple pomace pectin precipitated but not washed by alcohol. J Food Sci 72:1–9

    Article  Google Scholar 

  • Happi Emaga T, Rabetafika N, Blecker CS, Paquot M (2012) Kinetics of the hydrolysis of polysaccharide galacturonic acid and neutral sugars chains from flaxseed mucilage. Biotechnol Agron Soc Environ 16(2):139–147

    Google Scholar 

  • HappiEmaga T, Ronkart SN, Robert C, Wathelet B, Paquot M (2008) Characterisation of pectins extracted from banana peels (Musa AAA) under different conditions using an experimental design. Food Chem 108:463–471

    Article  CAS  Google Scholar 

  • Iacomini M, Serrato RV, Sassaki GL, Lopes L, Buchi DF, Gorin PAJ (2005) Isolation and partial characterization of a pectic polysaccharide from the fruit pulp of Spondias cytherea and its effect on peritoneal macrophage activation. Fitoterapia 76:676–683

    Article  CAS  Google Scholar 

  • Jiang YDY, Zhu X, Xiong H, Woo MW, Hu J (2012) Physicochemical and comparative properties of pectins extracted from Akebia trifoliata var. australis peel. Carbohydr Polym 87:1663–1669

    Article  CAS  Google Scholar 

  • Joye DD, Luzio GA (2000) Process for selective extraction of pectins from plant material by differential pH. Carbohydr Polym 43:337–342

    Article  CAS  Google Scholar 

  • Kalapathy U, Proctor A (2001) Effect of acid extraction and alcohol precipitation conditions on the yield and the purity of soy hull pectin. Food Chem 73:393–396

    Article  CAS  Google Scholar 

  • Kjoniksen AL, Hiorth M, Nystrom B (2005) Association under shear flow in aqueous solutions of pectin. Eur Polym J 41:761–771

    Article  CAS  Google Scholar 

  • Kliemann E, Simas KND, Amante ER, Prudêncio ES, Teofilo RF, Ferreira MMC, Amboni RDMC (2009) Optimization of pectin acid extraction from passion fruit peel (Passiflora edulis flavicarpa) using response surface methodology. Int J Food Sci Tech 44:476–483

    Article  CAS  Google Scholar 

  • Kulkarni SG, Vijayanand P (2010) Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f. flavicarpa L.) LWT-Food. Sci Technol 43:1026–1031

    CAS  Google Scholar 

  • Kumar A, Chauhan GS (2010) Extraction and characterization of pectin from apple pomace and its evaluation as lipase (steapsin) inhibitor. Carbohydr Polym 82:454–459

    Article  CAS  Google Scholar 

  • Levigne S, Ralet MC, Thibault JF (2002) Characterization of pectins extracted from fresh sugar beet under different conditions using an experimental design. Carbohydr Polym 49:145–153

    Article  CAS  Google Scholar 

  • Monsoor MA, Proctor A (2001) Preparation and Functional Properties of Soy Hull Pectin. J Am Oil Chem Soc 78(7):709–713

    Article  CAS  Google Scholar 

  • Mort AJ, Qui F, Maness NO (1993) Determination of the pattern of methyl esterification in pectin. Distribution of contiguous non esterified residues. Carbohydr Res 247:21–35

    Article  CAS  Google Scholar 

  • Nangia-Makker P, HoganV HY, Baccarini S, Tait L, Bresalier R, Raz A (2002) Inhibition of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus pectin. J Natl Cancer Inst 94:1854–1862

    Article  CAS  Google Scholar 

  • O’Donoghue EM, Somerfield SD (2008) Biochemical and rheological properties of gelling pectic isolates from buttercup squash fruit. Food Hydrocoll 22:1326–1336

    Article  Google Scholar 

  • Oosterveld A, Beldman G, Schols HA, Voragen AGJ (2000) Characterization of arabinose and ferulic acid rich pectic polysaccharides and hemicelluloses from sugar beet pulp. Carbohydr Res 328:185–197

    Article  CAS  Google Scholar 

  • Pagan J, Ibarz A (1999) Extraction and rheological properties of pectin from fresh peach pomace. J Food Eng 39:193–201

    Article  Google Scholar 

  • Pagan J, Ibarz A, Llorca M, Pagan A, Barbosa-Canovas GV (2001) Extraction and characterization of pectin from stored peach pomace. Food Res Int 34:605–612

    Article  CAS  Google Scholar 

  • Ptichkina NM, Markina OA, Rumyantseva GN (2008) Pectin extraction from pumpkin with the aid of microbial enzymes. Food Hydrocoll 22:192–195

    Article  CAS  Google Scholar 

  • Rolin C (2002) Commercial pectin preparations. In: Seymour GB, Knox JP (eds) Pectins and their manipulation. Blackwell, London, pp 222–241

    Google Scholar 

  • Rungrodnimitchai S (2011) Novel source of pectin from young sugar palm by microwave assisted extraction. Procedia Food Sci 1:1553–1559

    Article  Google Scholar 

  • Singthong J, Ningsanonda S, Cuib SW, Goff HD (2005) Extraction and physicochemical characterization of Krueo Ma Noy pectin. Food Hydrocoll 19:793–801

    Article  CAS  Google Scholar 

  • Voragen ACJ, Rolin C, Marr BU (2003) Pectins. In: Voragen ACJ, Rolin C, Marr BU, Challen I, Riad A, Lebabar R (eds) Polysaccharides, 6th edn. Wiley-VCH, New York, pp 189–195

    Google Scholar 

  • Wai WW, Alkarkhi AFM, Easa AM (2010) Effect of extraction conditions on yield and degree of esterification of durian rind pectin: an experimental design. J Food Bioprod Process 88:209–214

    Article  CAS  Google Scholar 

  • Wang XS, Liu L, Fang JN (2005) Immunological activities and structure of pectin from Centella asiatica. Carbohydr Polym 60:95–101

    Article  CAS  Google Scholar 

  • Willats WGT, Knox JP, Mikkelsen JD (2006) Pectin: New insights into an old polymer are starting to gel. Trends Food Sci Tech 17:97–104

    Article  CAS  Google Scholar 

  • Yapo BM, Robert C, Etienne I, Wathelet B, Paquot M (2007) Effect of extraction conditions on the yield, purity and surface properties of sugar beet pulp pectin extracts. Food Chem 100:1356–1364

    Article  CAS  Google Scholar 

  • Zhang J, Wang Z-W, Yu W-J, Wu J-H (2011) Pectins from Canna edulis Ker residue and their physicochemical characterization. Carbohydr Polym 83:210–216

    Article  CAS  Google Scholar 

  • Ziyada AK, Elhussien SA (2008) Physical and chemical characteristics of CitrulluslanatusVar Colocynthoide seed oil. J Phys Sci 19(2):69–75

    CAS  Google Scholar 

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Authors and Affiliations

  1. Arid Land Agriculture Department, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, P.O. Box 80208, Jeddah, 21589, Saudi Arabia

    Mohamed Korish

  2. Department of Food and Dairy Science &Technology, Faculty of Agriculture, Damanhour University, Damanhour, 22516, Egypt

    Mohamed Korish

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  1. Mohamed Korish
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Correspondence to Mohamed Korish.

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Korish, M. Potential utilization of Citrullus lanatus var. Colocynthoides waste as a novel source of pectin. J Food Sci Technol 52, 2401–2407 (2015). https://doi.org/10.1007/s13197-014-1277-y

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  • DOI: https://doi.org/10.1007/s13197-014-1277-y

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