Abstract
A method based on dispersive liquid-liquid microextraction (DLLME) was developed for the quantitative extraction of Ochratoxin A (OTA) from raisin samples. The influence of various parameters on the recovery of OTA such as type and volume of DLLME extractant, centrifuging and sonication time, also volume of deionized water was investigated. Recovery values under the optimum conditions were between 68.6 and 85.2 %, the inner and intra-day precision expressed as relative standard deviation (RSD%, n = 3), were less than 15 % at spiking levels of 2.5–30 μg kg−1. Linearity was studied from 0.5 to 30 μg L−1, and the limits of detection (LOD) and quantification (LOQ) were 0.7 and 2.0 μg kg−1, respectively. Real samples were analyzed by DLLME method and compared with confirmative immunoaffinity Column Chromatography (IAC) clean-up. Low cost, simplicity of operation, speed and minimum consumption of organic solvent were the main advantages of proposed method. The mean contamination of samples was 0.88 μg kg−1 that was lower than European Legal Limit.
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References
Amézqueta S, Schorr-Galindo S, Murillo-Arbizu M, González-Peñas E, López de Cerain A, Guiraud J (2012) OTA-producing fungi in foodstuffs: a review. Food Control 26:259–268
Arroyo-Manzanares N, Gámiz-Gracia L, García-Campaña AM (2012) Determination of ochratoxin a in wines by capillary liquid chromatography with laser induced fluorescence detection using dispersive liquid-liquid microextraction. Food Chem 135:368–372
Campone L, Piccinelli AL, Celano R, Rastrelli L (2011) Application of dispersive liquid–liquid microextraction for the determination of aflatoxins B1, B2, G1 and G2 in cereal products. J Chromatogr A 1218:7648
Covarelli L, Beccari G, Marini A, Tosi L (2012) A review on the occurrence and control of ochratoxigenic fungal species and ochratoxin A in dehydrated grapes, non-fortified dessert wines and dried vine fruit in the Mediterranean area. Food Control 26:347–356
Fang Y, Zhang A, Wang H, Li H, Zhang Z, Chen S, Luan L (2010) Health risk assessment of trace elements in Chinese raisins produced in Xinjiang province. Food Control 21:732–739
Hui YH (2008) Handbook of fruits and fruit processing. Wiley
Karami-Osboo R, Mirabolfathy M, Kamran R, Shetab-Boushehri M, Sarkari S (2012) Aflatoxin B1 in maize harvested over 3 years in Iran. Food Control 23:271–274
Karami-Osboo R, Maham M, Miri R, AliAbadi MHS, Mirabolfathy M, Javidnia K (2013) Evaluation of dispersive liquid–liquid microextraction–HPLC–UV for Determination of Deoxynivalenol (DON) in wheat flour. Food Anal Methods 6:176–180
Lapera L, Avellone G, Turco VL, Di Bella G, Agozzino P, Dugo G (2008) Influence of roasting and different brewing processes on the ochratoxin A content in coffee determined by high-performance liquid chromatography-fluorescence detection (HPLC-FLD). Food Addit Contam 25:1257–1263
Li P, Zhang Z, Zhang Q, Zhang N, Zhang W, Ding X, Li R (2012) Current development of microfluidic immunosensing approaches for mycotoxin detection via capillary electromigration and lateral flow technology. Electrophoresis 33:2253–2265
Li P, Zhang Z, Hu X, Zhang Q (2013) Advanced hyphenated chromatographic-mass spectrometry in mycotoxin determination: current status and prospects. Mass Spectrom Rev. doi:10.1002/mas.21377
MacDonald SJ, Anderson S, Brereton P, Wood R (2003) Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit, and dried figs by immunoaffinity column cleanup with liquid chromatography: interlaboratory study. J AOAC Int 86:1164–1171
Maham M, Karami-Osboo R, Kiarostami V, Waqif-Husain S (2012) Novel Binary Solvents-Dispersive Liquid—Liquid Microextraction (BS-DLLME) method for determination of patulin in apple juice using high-performance liquid chromatography. Food Anal Methods:1–6
Merdivan M, Mine AH (2012) Development of new dispersive liquid–liquid microextraction technique for the identification of zearalenone in beer. Anal Methods 4:4129–4134
Meyvaci K, Altindisli A, Aksoy U, Eltem R, Turgut H, Arasiler Z, Kartal N (2005) Ochratoxin A in sultanas from Turkey I: survey of unprocessed sultanas from vineyards and packing-houses. Food Addit Contam 22:1138–1143
Palumbo J, O’Keeffe T, Vasquez S, Mahoney N (2011) Isolation and identification of ochratoxin A-producing Aspergillus section Nigri strains from California raisins. Lett Appl Microbiol 52:330–336
Sugita-Konishi Y, Tanaka T, Nakajima M, Fujita K, Norizuki H, Mochizuki N, Takatori K (2006) The comparison of two clean-up procedures, multifunctional column and immunoaffinity column, for HPLC determination of ochratoxin A in cereals, raisins and green coffee beans. Talanta 69:650–655
Varga J, Kocsubé S, Koncz Z, Téren J (2006) Mycobiota and ochratoxin A in raisins purchased in Hungary. Acta Aliment 35:289–294
Víctor-Ortega MD, Lara FJ, García-Campaña AM, Olmo-Iruela M (2013) Evaluation of dispersive liquid-liquid microextraction for the determination of patulin in apple juices using micellar electrokinetic capillary chromatography. Food Control 31:353–358
Walker R (2002) Risk assessment of ochratoxin: current views of the European Scientific Committee on Food, the JECFA and the Codex Committee on Food Additives and Contaminants. Mycotoxins Food Saf:249–255
Walker R, Larsen JC (2005) Ochratoxin A: previous risk assessments and issues arising. Food Addit Contam 22:6–9
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Karami-Osboo, R., Miri, R., Javidnia, K. et al. A validated dispersive liquid-liquid microextraction method for extraction of ochratoxin A from raisin samples. J Food Sci Technol 52, 2440–2445 (2015). https://doi.org/10.1007/s13197-013-1215-4
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DOI: https://doi.org/10.1007/s13197-013-1215-4