Abstract
Medicinal plants are at great interest to the researcher in the field of biotechnology, as most of the drug industries depend in medicinal plants for the production of pharmaceutical compounds. Plants are the traditional sources for many chemicals used as pharmaceutical biochemicals, fragrances, food colours and flavours in different countries especially in India. Most herbal medicines and their derivative products were often prepared from crude plant extracts, which comprise a complex mixture of different phytochemical constituents (plant secondary metabolites). The chemical features of these constituents differ considerably among different species. GC-MS method used for the analysis of the obtained extracts can be an interesting tool for testing the amount of some active principles in herbs used in cosmetic, drugs, pharmaceutical or food industry. The aim of this study was to carry out for identification of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of ethanolic extract was done by standard protocol using the equipment Thermo GC-Trace Ultra Version: 5.0, Thermo MS DSQ II. The GC-MS analysis revealed the presence of various compounds like piperine, octodeconoic acids, hexadecanoic acid and squalene in the ethanolic extract of Evolvulus alsinoides. Hence, the Evolvulus alsinoides may have chemopreventive, anticancer, anti-microbial activity, antioxidant and antidiabetic activity due to the presence of secondary metabolites in the ethanolic extract. Due to the presence of esters which can be used as a flavoring agent in food industries. These findings support the traditional use of Evolvulus alsinoides in various disorders. Further studies are needed to isolate active principle of the extract as well as to elucidate their exact mechanism of action in various disorders.
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References
Abirami P, Rajendran A (2011) GC-MS determination of bioactive compounds of Indigofera aspalathoides. J Nat Prod Plant Resour 1(4):126–130
Atal S, Agrawal PR, Vyas S, Phadnis P, Rai N (2012) Evaluation of the effect of piperine per se on blood glucose level in alloxan-induced diabetic mice. Acta Pol Pharm-Drug Res 69(5):965–969
Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, Tripathi PC et al (2003) Screening of antioxidant activity of some three Indian medicinal plants traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol 84(2–3):131–138
Austin DF (2008) Evolvulus alsinoides (Convolvulaceae): an American herb in the old World. J Ethnopharmacol 117:185–198
Biswas S, Bhattacharyya J, Dutta AG (2005) Oxidant induced injury of erythrocyte-role of green tea leaf and ascorbic acid. Mol Cell Biochem 276:205–210
Cragg GM, Newman DJ (2005) A continuing source of novel drug leads. Pure Appl Chem 77:7–24
Daniel FA (2008) Review of Evolvulus alsinoides (Convolvulaceae): an American herb in the old world. J Ethnopharmacol 117:185–198
Deng SX, Wang DC, Wang MD et al (1964) The cardiac effect of Periploca sepium. Acta Pharm Sin 11:75
El Hamass R, Idaomar M, Alonso-Moraga M, Serrano AM (2003) Antimutagenic properties of bell and black peppers. Food Chem Toxicol 41(1):41–47
Ezhilan BP, Neelamegam R (2012) GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. Pharmacogn Res 4(1):11–14
Foresti ML, Errazu A, Ferreira M (2005) Effect of several reaction parameters in the solvent-free ethyl oleate synthesis using Candida rugosa lipase immobilised on polypropylene. Biochem Eng J 25(1):69–77
Galati G, Brien PJ (2004) Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anti-cancer properties. Free Radic Biol Med 37:287–303
Hazarika S, Goswami N, Dutta N, Hazarika A (2002) Ethyl oleate synthesis by porcine pancreatic lipase in organic solvent. Chem Eng J 85(1):61–68
Hedeji I, Xu JP (1988) Pregnane glycoside from an antitumour fraction of Periploca sepium. Phytochemistry 27:1173
Kala SMJ, Balasubramanian T, Soris PT, Mohan VR (2011) GC-MS determination of bioactive components of Eugenia singampattiana Bedd. Int J ChemTech Res 3(3):1534–1537
Karimi E, Jaafar HZE (2011) HPLC and GC-MS determination of bioactive compounds in microwave obtained extracts of three varieties of Labisia pumila benth. Molecules 16:6791–6805
Katerere FR, Grev AI, Nash RJ, Waigh RD (2003) Antimicrobial activity of pentacyclic triterpenes isolated from African combretaceae. Phytochemistry 63:81–88
Li S, Wang C, Wang M, Li W, Matsumoto K, Tang Y (2007) Antidepressant like effects of piperine in chronic mild stress treated mice and its possible mechanisms. Life Sci 80(15):1373–1381
Madhavan V, Yoganarasimhan SN, Gurudeva MR (2008) Pharmacognostical studies on Sankhapushphi (Convolvulus microphyllus Sieb. Ex Spreng. and Evolvulus alsinoides (L.) L. Indian J Trad Knowl 7(4):529–541
Mahato SB, Sen S (1997) Advances in triterpenoid research-1990–1994. Phytochemistry 44:115–123
Martiinez-Ruiz A, Garcia HS, Saucedo-Castaneda G, Favela-Torres E (2008) Organic phase synthesis of ethyl oleate using lipases produced by solid-state fermentation. Appl Biochem Biotechnol 151(2–3):393–401
Matsuda H, Ninomiya K, Morikawa T, Yasuda D, Yamaguchi I, Yoshikawa M (2008) Protective effects of amide constituents from the fruit of Piper chaba on D-galactosamine/TNF-alpha-induced cell death in mouse hepatocytes. Bioorg Med Chem Lett 18(6):2038–2042
Mu YM, Yanass T et al (2001) Saturated FFAs, palmitic acid and stearic acid, induced apoptosis in human granulose cells. J Endocrinol 142:3590–3597
Ponnamma SU, Manjunath K (2012) GC-MS Analysis of phytocomponents in the methanolic extract of Justicia wynaadensis (nees) T. anders. Int J Pharm Bio Sci 3(3):570–576
Prakash UN, Srinivasan K (2010) Gastrointestinal protective effect of dietary spices during ethanol-induced oxidant stress in experimental rats. Appl Physiol Nutr Metab 35(2):134–141
Radzi SM, Basri M, Salleh AB, Ariff A, Mohamad R, Rahman MBAR, Rahman RNZRA (2006) Optimization study of large-scale enzymatic synthesis of ethyl oleate, a liquid wax ester, by response surface methodology. J Chem Technol Biotechnol 81:374–380
Rani LS, Mohan VR, Regini GS, Kalidass C (2009) GC-MS analysis of ethanolic extract of Pothos scandens leaf. J Herb Medi Toxicol 3:159–160
Rao CV, Newmark HL, Reddy BS (1998) Chemopreventive effect of squalene on colon cancer. Carcinogenesis 19:287–297
Rauscher FM, Sanders RA, Watkins JB (2000) Effects of piperine on antioxidant pathways in tissues from normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 14:329–334
Rosemeyer J, Bodoprost H (2007) Analysis of phenacylester derivatives of fatty acids from human skin surface sebum by reversed-phase HPLC: chromatographic mobility as a function of physico-chemical properties. Int J Mol Sci 8:1111–1124
Scortichini M, Rossi PM (1991) Preliminary in vitro evaluation of antimicrobial activity of terpenes and terpenoids towards Erwinia amylovora (Burrill). J Appl Bact 71:109–112
Sethiya NK, Nahata A, Mishra SH, Dixit VK (2009) An update on shankhpushpi, a cognition-boosting ayurvedia medicine. J Chin Integr Med 7(11):1001–1022
Sharma PV (1983) Dravyaguna vijinana, (in Hindi) (Showkambha, Varanasi). Pp:5
Shi QH, Ma YM et al (2005) Preliminary study on chemical composition and insecticidal activity of root bark from Periploca sepium. Acta Agric Boreali-occidentalis Sin 14:141–144
Sunitha S, Nagaraj M, Varalakshmi P (2001) Hepatoprotective effect of lupeol and lupeol linoleate on tissue antioxidant defence system in cadmium-induced hepatotoxicity in rats. Fitoterapia 72(5):516–23
Ukiva M, Akihisa T, Tokuda H, Suzuki H, Mukainaka T, Ichiishi E (2002) Antitumor promoting effects and cytotoxic activity against human cancer lines of triterpene diols and triols from edible chrysanthemum flowers. Cancer Lett 177:7–12
Uma B, Prabhakar K, Rajendran S, Sarayu LY (2009) Studies on GC/MS spectroscopic analysis of some bioactive antimicrobial compounds from Cinnamomum zeylanicum. J Med Plants 8(31):125–131
Vijayakumar RS, Nalini N (2006) Piperin, an active principle from Piper nigrum, modulates hormonal and apo lipoprotein profiles in hyperlipidemic rats. J Basic Clin Physiol Pharmacol 17(2):71–86
Vuorela P, Leinonen M, Saikku P, Tammela P, Rouha JP, Wennberg T, Vuorela H (2004) Curr Med Chem 11:1375–1389
Wang L, Zhao D, Zhang Z, Zuo C, Zhang Y, Pei YQ, Lo YQ (1999) Trial of antiepilepsirine (AES) in children with epilepsy. Brain Dev 21(1):36–40
Zhang QX, Wang QL, Han JH (1995) Effect of fatty oil in Periploca sepium on neural system in mice. Xi’an Med Univ 16:43–44
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Gomathi, D., Kalaiselvi, M., Ravikumar, G. et al. GC-MS analysis of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides (L.) L. J Food Sci Technol 52, 1212–1217 (2015). https://doi.org/10.1007/s13197-013-1105-9
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DOI: https://doi.org/10.1007/s13197-013-1105-9