Abstract
The present study aims to maximize the extraction of polyphenols from ginger (Zingiber officinale) through the statistical optimization of three influential process parameters ethanol (EtOH) proportion (%), temperature (°C) and extraction time (min). Response Surface Methodology (RSM) was employed to design experiments and study the interaction effects of these parameters on the extraction process. Analysis of Variance (ANOVA) was used for the analysis of regression coefficient, prediction of equation and case statistics. The optimum conditions for the maximum yield of polyphenols from each gram of ginger were found to be 75 % aqueous EtOH, 40 °C temperature and extraction time of 60 min respectively. The order of relative importance of these three parameters was: EtOH > time > temperature. Antioxidant activity of the extracted polyphenols using optimized parameters was also determined by DPPH assay. DPPH radical scavenging activity of ginger extract was compared with Vitamin C and butyl hydroxy toluene (BHT). Finally, this study revealed a cost effective analytical model to maximize the extraction of polyphenols from ginger with higher antioxidant activity. It was also concluded that at lower concentration ethanolic extract of ginger possess high antioxidant activity in comparison with synthetic antioxidants like vitamin C or BHT and thus it can be applicable as potent natural antioxidant in food and pharmaceutical industries for the preparation of functional food.
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Acknowledgement
The authors would like to express their gratitude to Prof. (Dr.) Gautam Biswas, Director, CSIR-Central Mechanical Engineering Research Institute for his kind permission to publish the paper. The authors are also grateful to Council of Scientific and Industrial Research, Govt. of India for the financial support. The help rendered by our scientific and technical staffs of CAMP, CSIR-CMERI, Durgapur is acknowledged. Sincere thanks to Mr. Dipanjan Sengupta and Mr. Sanjeeb Sutradhar for their support during the preparation of this manuscript.
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Mukherjee, S., Mandal, N., Dey, A. et al. An approach towards optimization of the extraction of polyphenolic antioxidants from ginger (Zingiber officinale). J Food Sci Technol 51, 3301–3308 (2014). https://doi.org/10.1007/s13197-012-0848-z
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DOI: https://doi.org/10.1007/s13197-012-0848-z