Abstract
Direct acidified cottage cheese has a limited shelf life of 10–12 days under refrigeration due to high moisture content (~75 %) and a relatively high pH (~5.0). This affects its widespread marketing and distribution. Hence, a study was undertaken to improve the shelf life of direct acidified cottage cheese using thymol, a phytophenolic natural antimicrobial agent. The effect of three different levels, i.e. 30, 40 and 50 ppm of 30 % thymol solution in butteroil on the physico-chemical, microbiological and organoleptic properties was studied at four-day interval during storage under refrigeration (4–5 °C). Promising results were obtained using 40 ppm thymol for inhibiting psychrotrophs, yeasts and molds as well as retarded the proteolysis in cottage cheese. Based on the effect on flavour of the fresh samples as well as the extent of changes in quality during storage, it was observed that addition of 40 ppm thymol enhanced the keeping quality of cottage cheese by 8 days compared to the control sample.
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References
ADSA (2004) Cottage cheese score card, 2003 rules. In: 83rd Annual Collegiate Dairy Products Evaluation Contest. Committee on Dairy Product Evaluation, American Dairy Science Association. 1111 N. Dunlap Avenue Savoy. IL 61874. USA. pp 16
Angevine NC (1959) Keeping quality of cottage cheese. J Dairy Sci 42:2015–2019
AOAC (2000) Association of official analytical chemists. Official methods of analysis. 17th edn. William Horwitz (ed). Vol. II. Assc Official Agric Chem, Washington DC
Conner DE, Beuchat LR (1984a) Sensitivity of heat stressed yeasts to essential oils of plants. Appl Environ Microbiol 47:229–233
Conner DE, Beuchat LR (1984b) Effects of essential oils of plants on growth of food spoilage yeasts. J Food Sci 49:429–434
Darvill AG, Albersheim P (1984) Phytoalexins and their elicitors: a defence against microbial infection in plants. Annu Rev Plant Physiol 34:243–275
Davidson PM (1997) Chemical preservatives and natural antimicrobial compounds. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food microbiology fundamentals and frontiers. ASM, New York, pp 520–556
Deeth HC, Fitzgerald CH (1976) Lipolysis in dairy products: a review. Aust J Dairy Technol 31:53–64
Emmons DB, Tuckey SL (1967) Cottage cheese and other cultured milk products. Has Pfizer & Co, Inc, New York
Evanston IL (1990) System of Statistics (SYSTAT). Evanston IL (ed), Wilkinson
Hammer KA, Carson CF, Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 86:985–990
Harmon LG, Smith CL (1956a) Spoilage organisms in cottage cheese. J Dairy Sci 39:9–15
Harmon LG, Smith CL (1956b) The influence of environment and processing in spoilage organisms in cottage cheese. J Dairy Sci 19:252–255
Helander IK, Alakomi HL, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ, von Wright A (1998) Characterization of the action of selected essential oil components on Gram negative bacteria. J Argil Chem 46:3590–3595
Houghtby GA, Maturin LJ, Koenig EK (1993) In: Marshall RT (ed) Microbiological count methods, 16th edn. American Public Health Association (APHA), Washington, pp 213–246
Hugo WB (1991) A review: a brief history of heat and chemical preservation and disinfection. J Appl Bacteriol 71:9–18
ISO 8586:1993E. Sensory analysis-methodology-general guidance. International Organization for Standardization, Geneva, Switzerland
ISO 8586–1:1993E. Sensory analysis-general guidance for the selection, training and monitoring of assessors. International Organization for Standardization, Geneva, Switzerland
ISO 8589:1985. Sensory analysis-general guidance for the design of test rooms. International Organization for Standardization, Geneva, Switzerland
Johnson P (1979) Cottage cheese: a primer for high quality and long shelf life. Am Dairy Rev 41:40O–40P
Juven BJ, Kanner J, Schved F, Weisslowicz H (1994) Factors that interest with the antibacterial action of thyme essential oil and its active constituent. J Appl Bacteriol 76:626–631
Katayama T, Nagai I (1960) Chemical significance of the volatile components of spices in the food preservative viewpoint. VI. Structure and antibacterial activity of terpenes. Bull Jpn Soc Sci Fisheries 26:29–32
Koga T, Hirota N, Takumi K (1999) Bactericidal activities of essential oils of basil and sage against a range of bacteria and the effect of these essential oils on Vibrio parahaemolyticus. Microbiol Res 154:267–273
Kokkini S, Karousou R, Dardioti A, Krigas N, Lanaras T (1997) Autumn essential oils of Greek oregano. Phytochemistry 44:883886
Kosikowski FV (1982) Cottage cheese. In: Kosikowski FV (ed) Cheese and fermented milk foods, 2nd edn. F. V. Kosikowski and Associates, New York, pp 109–143
Leung AY (1978) Encyclopedia of common natural ingredients used in foods, drugs and cosmetics. Wiley, New York, pp 309–311
Luck H, Mosteret JF, Husmann RA (1977) Shelf life of perishable dairy products. S Afr J Dairy Technol 9:25–27
Makhal S, Kanawjia SK (2003a) Preservation of cottage cheese: a review. Indian J Dairy Sci 56:1–12
Makhal S, Kanawjia SK (2003b) Microbial metabolites in preserving dairy products—an overview. J Food Dairying Home Sci 23:157–171
Makhal S, Kanawjia SK (2003c) Phytophenols in preservation of dairy products: fads and fantasy to facts and figures. Indian J Dairy Biosci 14:82–87
Makhal S, Mandal S, Kanawjia SK (2003) MicroGARD™ in food preservation: a shifted paradigm towards natural preservatives. Indian J Dairy Biosci 14:70–78
Makhal S, Giri A, Kanawjia SK (2011) Effect of κ-carrageenan and tetrasodium pyrophosphate on the yield of direct acidified cottage cheese. J Food Sci Technol. doi:10.1007/s13197-011-0438-5
Robinson RK (1981) Dairy microbiology. Vol. 2. Elsevier Science Publishers Ltd, London
Robinson RK (1995) A colour guide to cheese and fermented milk. Chapman and Hall, London
Scafer JG (1958) Cottage cheese sanitation practice. Am Milk Rev 20:42–44
Shakeel-Ur-Rehman, Farkye NY, Drake MA (2003) Reduced fat cheddar cheese from a mixture of cream and liquid milk protein concentrate. Int Dairy J 56:94–98
Sikkema J, de Bont JAM, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59:201–222
Suriyarachchi RV, Fleet HG (1981) Occurrence and growth of yeast in yogurts. Appl Environ Microbiol 42:574–577
Thuille N, Fille M, Nagl M (2003) Bactericidal activity of herbal extracts. Int J Hyg Environ Health 206:217–221
Ultee A, Kets EPW, Smid EJ (1999) Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 65:4606–4610
USDA (1953) Cheese varieties and descriptions. USDA Agriculture Handbook 54. US Department of Agriculture, Washington
Vas K (1953) Mechanisms of antimicrobial action-interference with the cytoplasmic membrane. Agrokém Talajt 2:1–16
Vazquez BI, Fente C, Franco CM, Vazquez MJ, Cepeda A (2001) Inhibitory effects of eugenol and thymol on Penicillium citrinum strains in culture media and cheese. Int J Food Microbiol 67:157–163
Walstra P, Jenness R (1984) Dairy chemistry and physics. John Wiley, New York
Wan J, Wilcock A, Coventry MJ (1998) The effect of essential oils of basil on the growth of Aeromonas hydrophila and Pseudomonas fluorescens. J Appl Microbiol 84:152–158
Wendorff WL, Wee C (1997) Effect of smoke and spice oils on growth of molds on oil-coated cheese. J Food Prot 60:153–156
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Makhal, S., Kanawjia, S.K. & Giri, A. Effectiveness of thymol in extending keeping quality of cottage cheese. J Food Sci Technol 51, 2022–2029 (2014). https://doi.org/10.1007/s13197-012-0715-y
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DOI: https://doi.org/10.1007/s13197-012-0715-y