Skip to main content
Log in

Effectiveness of thymol in extending keeping quality of cottage cheese

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Direct acidified cottage cheese has a limited shelf life of 10–12 days under refrigeration due to high moisture content (~75 %) and a relatively high pH (~5.0). This affects its widespread marketing and distribution. Hence, a study was undertaken to improve the shelf life of direct acidified cottage cheese using thymol, a phytophenolic natural antimicrobial agent. The effect of three different levels, i.e. 30, 40 and 50 ppm of 30 % thymol solution in butteroil on the physico-chemical, microbiological and organoleptic properties was studied at four-day interval during storage under refrigeration (4–5 °C). Promising results were obtained using 40 ppm thymol for inhibiting psychrotrophs, yeasts and molds as well as retarded the proteolysis in cottage cheese. Based on the effect on flavour of the fresh samples as well as the extent of changes in quality during storage, it was observed that addition of 40 ppm thymol enhanced the keeping quality of cottage cheese by 8 days compared to the control sample.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • ADSA (2004) Cottage cheese score card, 2003 rules. In: 83rd Annual Collegiate Dairy Products Evaluation Contest. Committee on Dairy Product Evaluation, American Dairy Science Association. 1111 N. Dunlap Avenue Savoy. IL 61874. USA. pp 16

  • Angevine NC (1959) Keeping quality of cottage cheese. J Dairy Sci 42:2015–2019

    Article  CAS  Google Scholar 

  • AOAC (2000) Association of official analytical chemists. Official methods of analysis. 17th edn. William Horwitz (ed). Vol. II. Assc Official Agric Chem, Washington DC

    Google Scholar 

  • Conner DE, Beuchat LR (1984a) Sensitivity of heat stressed yeasts to essential oils of plants. Appl Environ Microbiol 47:229–233

    CAS  Google Scholar 

  • Conner DE, Beuchat LR (1984b) Effects of essential oils of plants on growth of food spoilage yeasts. J Food Sci 49:429–434

    Article  Google Scholar 

  • Darvill AG, Albersheim P (1984) Phytoalexins and their elicitors: a defence against microbial infection in plants. Annu Rev Plant Physiol 34:243–275

    Article  Google Scholar 

  • Davidson PM (1997) Chemical preservatives and natural antimicrobial compounds. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food microbiology fundamentals and frontiers. ASM, New York, pp 520–556

    Google Scholar 

  • Deeth HC, Fitzgerald CH (1976) Lipolysis in dairy products: a review. Aust J Dairy Technol 31:53–64

    CAS  Google Scholar 

  • Emmons DB, Tuckey SL (1967) Cottage cheese and other cultured milk products. Has Pfizer & Co, Inc, New York

    Google Scholar 

  • Evanston IL (1990) System of Statistics (SYSTAT). Evanston IL (ed), Wilkinson

  • Hammer KA, Carson CF, Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 86:985–990

    Article  CAS  Google Scholar 

  • Harmon LG, Smith CL (1956a) Spoilage organisms in cottage cheese. J Dairy Sci 39:9–15

    Google Scholar 

  • Harmon LG, Smith CL (1956b) The influence of environment and processing in spoilage organisms in cottage cheese. J Dairy Sci 19:252–255

    Google Scholar 

  • Helander IK, Alakomi HL, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ, von Wright A (1998) Characterization of the action of selected essential oil components on Gram negative bacteria. J Argil Chem 46:3590–3595

    Article  CAS  Google Scholar 

  • Houghtby GA, Maturin LJ, Koenig EK (1993) In: Marshall RT (ed) Microbiological count methods, 16th edn. American Public Health Association (APHA), Washington, pp 213–246

    Google Scholar 

  • Hugo WB (1991) A review: a brief history of heat and chemical preservation and disinfection. J Appl Bacteriol 71:9–18

    Article  CAS  Google Scholar 

  • ISO 8586:1993E. Sensory analysis-methodology-general guidance. International Organization for Standardization, Geneva, Switzerland

  • ISO 8586–1:1993E. Sensory analysis-general guidance for the selection, training and monitoring of assessors. International Organization for Standardization, Geneva, Switzerland

  • ISO 8589:1985. Sensory analysis-general guidance for the design of test rooms. International Organization for Standardization, Geneva, Switzerland

  • Johnson P (1979) Cottage cheese: a primer for high quality and long shelf life. Am Dairy Rev 41:40O–40P

    Google Scholar 

  • Juven BJ, Kanner J, Schved F, Weisslowicz H (1994) Factors that interest with the antibacterial action of thyme essential oil and its active constituent. J Appl Bacteriol 76:626–631

    Article  CAS  Google Scholar 

  • Katayama T, Nagai I (1960) Chemical significance of the volatile components of spices in the food preservative viewpoint. VI. Structure and antibacterial activity of terpenes. Bull Jpn Soc Sci Fisheries 26:29–32

    Article  CAS  Google Scholar 

  • Koga T, Hirota N, Takumi K (1999) Bactericidal activities of essential oils of basil and sage against a range of bacteria and the effect of these essential oils on Vibrio parahaemolyticus. Microbiol Res 154:267–273

    Article  CAS  Google Scholar 

  • Kokkini S, Karousou R, Dardioti A, Krigas N, Lanaras T (1997) Autumn essential oils of Greek oregano. Phytochemistry 44:883886

    Article  Google Scholar 

  • Kosikowski FV (1982) Cottage cheese. In: Kosikowski FV (ed) Cheese and fermented milk foods, 2nd edn. F. V. Kosikowski and Associates, New York, pp 109–143

    Google Scholar 

  • Leung AY (1978) Encyclopedia of common natural ingredients used in foods, drugs and cosmetics. Wiley, New York, pp 309–311

    Google Scholar 

  • Luck H, Mosteret JF, Husmann RA (1977) Shelf life of perishable dairy products. S Afr J Dairy Technol 9:25–27

    Google Scholar 

  • Makhal S, Kanawjia SK (2003a) Preservation of cottage cheese: a review. Indian J Dairy Sci 56:1–12

    Google Scholar 

  • Makhal S, Kanawjia SK (2003b) Microbial metabolites in preserving dairy products—an overview. J Food Dairying Home Sci 23:157–171

    Google Scholar 

  • Makhal S, Kanawjia SK (2003c) Phytophenols in preservation of dairy products: fads and fantasy to facts and figures. Indian J Dairy Biosci 14:82–87

    Google Scholar 

  • Makhal S, Mandal S, Kanawjia SK (2003) MicroGARD™ in food preservation: a shifted paradigm towards natural preservatives. Indian J Dairy Biosci 14:70–78

    Google Scholar 

  • Makhal S, Giri A, Kanawjia SK (2011) Effect of κ-carrageenan and tetrasodium pyrophosphate on the yield of direct acidified cottage cheese. J Food Sci Technol. doi:10.1007/s13197-011-0438-5

  • Robinson RK (1981) Dairy microbiology. Vol. 2. Elsevier Science Publishers Ltd, London

    Google Scholar 

  • Robinson RK (1995) A colour guide to cheese and fermented milk. Chapman and Hall, London

    Google Scholar 

  • Scafer JG (1958) Cottage cheese sanitation practice. Am Milk Rev 20:42–44

    Google Scholar 

  • Shakeel-Ur-Rehman, Farkye NY, Drake MA (2003) Reduced fat cheddar cheese from a mixture of cream and liquid milk protein concentrate. Int Dairy J 56:94–98

    Article  CAS  Google Scholar 

  • Sikkema J, de Bont JAM, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59:201–222

    CAS  Google Scholar 

  • Suriyarachchi RV, Fleet HG (1981) Occurrence and growth of yeast in yogurts. Appl Environ Microbiol 42:574–577

    CAS  Google Scholar 

  • Thuille N, Fille M, Nagl M (2003) Bactericidal activity of herbal extracts. Int J Hyg Environ Health 206:217–221

    Article  Google Scholar 

  • Ultee A, Kets EPW, Smid EJ (1999) Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 65:4606–4610

    CAS  Google Scholar 

  • USDA (1953) Cheese varieties and descriptions. USDA Agriculture Handbook 54. US Department of Agriculture, Washington

    Google Scholar 

  • Vas K (1953) Mechanisms of antimicrobial action-interference with the cytoplasmic membrane. Agrokém Talajt 2:1–16

    CAS  Google Scholar 

  • Vazquez BI, Fente C, Franco CM, Vazquez MJ, Cepeda A (2001) Inhibitory effects of eugenol and thymol on Penicillium citrinum strains in culture media and cheese. Int J Food Microbiol 67:157–163

    Article  CAS  Google Scholar 

  • Walstra P, Jenness R (1984) Dairy chemistry and physics. John Wiley, New York

    Google Scholar 

  • Wan J, Wilcock A, Coventry MJ (1998) The effect of essential oils of basil on the growth of Aeromonas hydrophila and Pseudomonas fluorescens. J Appl Microbiol 84:152–158

    Article  CAS  Google Scholar 

  • Wendorff WL, Wee C (1997) Effect of smoke and spice oils on growth of molds on oil-coated cheese. J Food Prot 60:153–156

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Apurba Giri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Makhal, S., Kanawjia, S.K. & Giri, A. Effectiveness of thymol in extending keeping quality of cottage cheese. J Food Sci Technol 51, 2022–2029 (2014). https://doi.org/10.1007/s13197-012-0715-y

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-012-0715-y

Keywords

Navigation