Abstract
Combined effect of lime and drying on bacteria of public health significance in Edible Oyster (Crassostrea madrasensis) from Munambam coastal belt (Kerala, India) were studied (without depuration). Samples were examined for Total Plate Count (TPC), Staphylococcus aureus (hygiene indicator), Total coliforms, Faecal coliforms, Escherichia coli, (faecal indicator) Faecal Streptococci (faecal indicator), Salmonella, Vibrio cholera and Listeria monocytogenes. The fresh oyster meat though did not confirm to the specifications laid by National shellfish sanitation programme (NSSP), after treatment with lime with and without drying found to show significant reduction in counts and meet the required standards. Prevalence of faecal indicators in the fresh sample indicated faecal pollution in the area. The isolation of potentially pathogenic bacteria, V. parahaemolyticus in fresh sample indicates high risk of people consuming and handling oysters in raw and semi processed form and also it may lead to cross contamination. The present study indicates that treatment with natural organic product like lime and simple preservation technique, drying can effectively reduce the bacterial load. The study also revealed that TPC of water and soil collected from the site from where oysters were collected was less than from the meat.
References
APHA (2001) In: Downes FP, Ito K (eds) Compendium of methods for the microbiological examination of foods. American Public Health Association, New York
Cook DW, Oleary P, Hunsucker JC, Sloan EM, Bowers JC, Blodgett RJ, Depaola A (2002) Vibrio vulnificus and Vibrio parahaemolyticus in U.S. retail shell oysters: a national survey from June 1998 to July 1999. J Food Prot 65:79–87
DePaola A, McLeroy S, McManus G (1997) Distribution of Vibrio vulnificus phage in oyster tissues and other estuarine habitats. Appl Environ Microbiol 63:2464–2467
Efiuvwevwere J, Ajiboye MO (1996) Control of microbiological quality and shelf-life of catfish (Clarias gariepinus) by chemical preservatives and smoking. J Appl Bacteriol 80:465–470
FDA (1998) Fish and fisheries products hazards and control guide, 2nd edn. Office of Seafood, CFSCAN, U.S. FDA, Public Health Service, Dept. Health and Human Dervices, Washingdon D.C
FDACFSAN (2005) National shellfish sanitation program: guide for the control of molluscan shellfish. Chapter IV: Naturally Occurring Pathogens
Gram L, Huss HH (2000) Fresh and processed fish and shellfish. In: Lund BM, Baird-Parker TC, Gould GW (eds) The microbiological safety and quality of food, vol 1. Aspen, Gaithersburg, pp 472–506
Hackney CR, Dicharry A (1988) Seafood borne bacterial pathogens of marine origin. Food Tech 42(3):104–109
Jay JM (1998) Modern food microbiology, 5th edn. Aspen, Gaithersburg
Joseph KG (2007) Listeria monocytogenes in marine products & Salmonella in marine products In: Mukundan MK, Balasubramaniam S (ed) Seafood quality assurance, Cift, training manual 1. Central Institute of Fisheries Technology, (ICAR), Matsyapuri, p.o., Cochin-29:60–64, 67–106
Karunasagar I, Karunasagar I, Venugopal MN, Nagesha CN (1987) Survival of Vibrio parahaemolyticus in estuarine water, seawater and in association with clams. Syst Appl Microbiol 9:316–319
Lakshmanan PT, Mathen C, Varma PRG, Iyer TSG (1984) Assessment of quality of fish landed at the Cochin Fisheries Harbour. Fish Technol 21:98–105
Lees D (2000) Viruses and bivalve shellfish. Int J Food Microbiol 59:81–116
Nickelson RI, McCarthy S, Finne G (2001) Fish, crustaceans, and precooked seafoods. In: Downes FP, Ito K (eds) Compendium of methods for the microbiological examination of foods, 4th edn. American Public Health Association, Washington, pp 497–505
Nambiar VN, Iyer KM (1990) Microbial quality of fish in retail trade in Cochin. Fish Technol 27:51–59
Potasman I, Paz A, Odeh M (2002) Infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective. Clin Infect Dis 233:1065–1068
Reilly PGA, Barle LE (1987) Depuration of farmed bivalves in Philippines. Infofish Marketing Digest 4:44–46
Richards GP (1988) Microbial purification of shellfish: a review of depuration and relaying. J Food Prot 51:218–251
Ruwa RK, Polk P (1994) Patterns of spat settlement recorded for tropical oyster Crassostrea cucullata (Borne 1778) and the barnacle, Balanus Amphitrite (Darwin 1854) in a mangrove creek. Trop Zool 1:121–131
Sanjeev S (2007) Determination of total bacterial count, Escherichia coli, faecal streptococci and Staphylococcus in fish., Vibrio parahaemolyticus in marine products and its isolation and identification &Vibrio cholera in seafoods and its isolation. In: Mukundan MK, Balasubramaniam S (ed) Seafood Quality Assurance, CIFT, training manual 1. Central Institute of Fisheries Technology, (ICAR), Matsyapuri, p.o., Cochin-29: 29–30,549-59
Tack JF, Vanden-Berghe, Polk P (1992) Ecomorphology of Crassostrea cucullata (Borne1778) (Ostreidae) in mangrove creek (Gazy Kenya) Hydrobiolopension feeding ascidians and jelly fish. Ophelia 41:329–344
Wood CD (1981) The prevention of losses in cured fish. FAO, Rome
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Hassan, F., Geethalakshmi, V., Jeeva, J.C. et al. Combined effect of lime (Citrus aurantitolia) and drying on reducing bacteria of public health significance in Edible Oyster (Crassostrea madrasensis). J Food Sci Technol 50, 203–207 (2013). https://doi.org/10.1007/s13197-011-0550-6
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DOI: https://doi.org/10.1007/s13197-011-0550-6