Abstract
Solid-liquid extraction of phytic acid (PA) from rice bran was optimized by the maximization of the yield using response surface methodology. A Box-Behnken design was used to monitor the effects of three processing parameters of extraction on the PA yield, including ratio of acid solution to raw material (mL/g), hydrochloric acid concentration (mol/L), and extraction time (h). The results showed that the optimal conditions were acid solution/raw material of 8.5:1 (mL/g), HCl concentration 0.62 mol/L and extraction time 5.5 h. Validation tests indicated that the actual yield of PA was (2.15 ± 0.02)% with RSD = 1.92% (n = 5) under the optimized conditions, which was in good agreement with predicted yield. Antioxidant assays suggested that the extracted PA had weaker DPPH, hydroxyl and superoxide free-radical-scavenging capabilities than vitamin C at the same concentration of 0.5 mg/mL.
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Financial supports for this work from the Education Bureau Project for Scientific Research of Zhejiang Province (No. Y200803745) and the Key Brainstorm Project for Science and Technology of Zhejiang Province (No. 2007 C12019) were gratefully acknowledged.
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Zhang, H.W., Bai, X.L. Optimization of extraction conditions for phytic acid from rice bran using response surface methodology and its antioxidant effects. J Food Sci Technol 51, 371–376 (2014). https://doi.org/10.1007/s13197-011-0521-y
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DOI: https://doi.org/10.1007/s13197-011-0521-y